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The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板，根据其鞭毛区与其它细菌高度同源性设计引物，利用PCR方法扩增鞭毛蛋白基因flic片段，将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定，再将其亚克隆与载体pGEx-kG分别双酶切和连接，构建重组表达载体pGEX-flic，并将其转化大肠杆菌工程菌BL21，进行原核表达。

Mom is a life master. When she bought a piece of flower cloth, she will use the big piece to make clothes and the fragment piece to make shoes. For the wasted paper boxes, she will use the new ones to store shoes, and the old ones to foster hen.

妈可真会过，扯块大花布，整块的做衣服，碎块的纳鞋底；要来的废纸盒，新的装鞋子，旧的养鸡，装满鸡屎的纸盒这才扔去。

Besides other fragment s of hemoglobin and other similar compound coming from the blood of animals, such as foraminifera, minute crustaceans and small worms.

此外还有其它一些来自动物血液的化合物碎片，诸如有孔虫、微小甲壳动物和微小蠕虫类。

A 1.2 kb fragment of the gyrB gene of 17 Gordonia strains including type strains were determined by direct sequencing of PCR amplified fragments. Total 25 strains (8 were retrieved from public database) of genus Gordonia form a distinct phyletic line in the gyrB-based tree and are separated from other closely-related species of genera to suborder Corynebacterineae.

在Gordonia菌种间之差异研究中则发现12种Gordonia gyrB基因序列相似度介於79.3%-97.2%，16S rDNA的序列相似度为96.2%-99.7%，可知Gordonia放线菌的16S rDNA保留性极高，较难利用此基因序列进行菌种间之区分。gyrB基因之变异程度无论是在菌属，菌种甚至各分离株间均较16S rRNA基因大。

A 717 base pairs long artificial gene fragment of PrV gE has been synthesized by using preferred codons in the highly expressed Pichia genes. Four groups of oligonucleotides were successively cloned in pMD18-T vector to obtain four large fragments.

通过人工合成基因的方法对编码gE蛋白的第29～268位氨基酸残基的核苷酸序列（717bp）进行改造，用P.pastoris高表达蛋白偏爱的密码子替换原有的核苷酸序列，氨基酸序列保持不变。

RESULTS suc2 gene fragment of about 1.5 kb was amplified and cloned. DNA sequencing confirmed that , compared with the published sequence, the cloned suc2 gene had correct sequ ence except for one point mutation, but that did not alter the amino acid sequence o f t he encoded peptide. By transformation of yeast cells with a constructed gene dis ruption vector, four yeast clones were obtained which showed correct auxotrophy. PCR and DNA sequencing indicated that these yeast clones had the expected genot ype.

结果用 PCR从酵母基因组DNA中扩增出大小约1.5 kb的suc2基因片段，序列测定表明除585位有一点突变外，所得suc2基因与文献报道相同，构建的定位突变载体导入酵母细胞后，得到4株营养型符合的突变体，PCR表明这些突变体中均有suc2基因的同源重组，取其中1株的PCR产物进行序列测定证实了同源重组的发生。

Results The serum antibody level reached to 0.71±0.08-0.77±0.06 after genetic vaccine pCD-HCV1(100 μg/mouse) were inoculated into the mice(n=12) three or four times while blank vector pCD-SRα1 could not induce the mice(n=8) to generate antibody response in same way.After the antibody levels in mice(n=8) immunized by pCD-HCV1 had ascended to peak value(0.71), there was no trend of descending during the following 18 weeks of detection(0.68±0.06-0.75±0.07). Specific fragment of HCV cDNA identified by polymerase chain reaction from DNA extracted from the muscles of the mice after pCD-HCV1 had beed inoculated three months.

结果 基因疫苗pCD-HCV1(100 μg/只)3-4次接种小鼠后(n=12)，血清抗体水平达0.71±0.08-0.77±0.06，空载体pCD-SRα1免疫鼠血清抗体阴性；免疫鼠(n=8)抗体水平达高峰后(0.71)，持续检测18周未见下降趋势(0.68±0.06-0.75±0.07)；pCD-HCV1免疫小鼠3个月后，用聚合酶链反应仍可从肌肉组织中扩增出特异性HCV cDNA片段；免疫鼠PBMC对HCV合成肽CP9、基因重组抗原C、E1刺激后均出现增殖反应，刺激指数分别为4.07±1.58、3.88±0.70和3.69±1.30，与pCD-SRα1免疫鼠相比，差异有显著性(P.001)。

If one fragment, one part of you...

如果一个碎片，你的一部分。。。

I miss you so much, But that is just a fragment of my imagination and fantasy.

我想念你这么多，但是这仅仅是一个片段，我的想像力和幻想。

If you can do this, you will have instilled some fragment of wisdom.

如果你能这样做，你就向他们灌输了一点智慧。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。