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By using Roche kit as standard, the amplification efficiency of different reagents were compared by Roche Light Cycler instrument for establishing real-time PCR reaction system. The primers were designed based on signature sequence of chromosomal DNA fragment and caf 1 gene in plasmid pMT1 of Y.pestis. And sensitivity and specificity assay were done with serial diluted Y. pestis EV76 strain and other bacterias. Then 275 Y.

采用SYBRGreenI随机掺入法,以Roche试剂盒为标准,比较两公司的DNA聚合酶,用鼠疫菌310 0 4建立荧光PCR反应体系;针对鼠疫耶尔森氏菌特异的染色体标识序列和质粒上F1抗原基因设计引物,检测其灵敏度和特异性,以盲测试验进行验证;在此基础上鉴定2 75株鼠疫耶尔森氏菌,并测试该法检测脏器污染模拟标本的灵敏度。

The fragment encoding the polypeptide of wheat MLO protein C terminus located in cytoplast was cloned into the vector pET-30a and expressed in the strain BL21 of E coli.

本研究将编码小麦MLO蛋白羧基端约130个氨基酸多肽的基因片断克隆到表达载体pET-30a中,在大肠杆菌中表达了该多肽的融合蛋白。

RESULTS: The polymerase chain reaction product was in concordance with target fragment. The analysis of the gene sequence proved it is the ALR gene. The overall length of the ALR gene was 378 bp, it was highly conservative in many species, for instance the Homo sapiens, the Mus musculus, the Bos Taurus and the Danio rerio.

结果:聚合酶链反应扩增结果与目的片段位置一致,测序结果表明为肝再生增强因子mRNA,基因全长378 bp,在人、牛、小鼠、斑马鱼等物种中高度保守。

I generally don't use those things because for a fragment of a sec you are defenseless.

我一般不使用这些东西都是手无寸铁的一个片段,因为你的几秒钟之内。

A fragment of phthalate dioxygenase gene was successfully amplified from the genus of Delftia for the first time using a set of degenerate primers.

以一对简并引物,首次在该属中扩增出编码邻苯二甲酸双加氧酶的基因片段。

Do not let the discussion fragment into a desultory conversation with no clear direction.

不要让讨论变得支离破碎,成为没有明确方向的漫谈。

Based on complicate data analysis we obtained experimental elastic scattering cross section of 〓He as well as double differential cross section and angle distribution of 〓He fragment from 〓He breakup reaction.

通过复杂的数据分析提取了〓He的弹性散射实验截面和〓He破裂反应产生的〓He的双微分截面及角分布,首次提供了〓He在〓Be靶上的中能破裂反应数据。

This makes the study of the area more difficulty.The sand reservoir clastic fragment, the characteristics of infillings, digenesis and the structure and evolution of pores have been studied by means of many techniques including of microscope, EMS, high pressure mercury intrusion and thin section.

应用显微镜下观察、电镜扫描、高压压汞及铸体等分析技术,对砂岩储层的碎屑组分、填隙物特征、成岩作用以及孔隙结构与孔隙演化进行了研究,尝试运用数理统计方法研究了储层非均质性,分析评价了储层物性特别是渗透率的非均质性。

In this study, we have also designed three primers to amplify Jiaozhou Bay dinoflagellate ribosomal RNA small subunit gene fragment.

另外,我们还设计了甲藻核糖体小亚基基因(ribosomal RNA small subunit gene,ssu rDNA)特异引物,对胶州湾表层海水甲藻遗传多样性进行了初步研究。

We investigated the composition of the dinoflagellate genus Symbiodinium in 22 species of scleractinian corals from 14 genera and eight families in Luhuitou fringing reef of Sanya, using polymerase chain reaction of the large subunit rDNA and restriction fragment length polymorphism.

本研究利用PCR技术扩增核糖体基因大亚基片段以及限制性片段长度多态性的方法,对海南三亚鹿回头岸礁的8科14属22种造礁石珊瑚的共生藻组成进行了研究。

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推荐网络例句

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?