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It was seen that strain 6055 generated three kinds of dextransucrases whose molecular weights were 151 kDa,142kDa and 117kDa,respectively.Among them,the former two ones were found both in supematants and cell wall,while the later was only found in the fermentation liquor.Strain DM 1-2 synthesized two kinds of dextransucrases,and their molecular weights were 183kDa and 142kDa,respectively.The former existed both in cell wall and out of cell,but the later only was located on cell surface.Strain PC 13 produced three kinds of dextransucrases,and their molecular weights were 148kDa, 138kDa and 115kDa,respectively.The former two ones were ectoenzyme while the later was linked to cell.Resembled to strain 6055,strain L4 produced two types of dextransucrases coexisting in supernatants and cell wall with molecular weights of 145 kDa and 136kDa,respectively,but the dextransucrase of 115kDa was only found in free-cells supernatants.

结果显示,菌株6055可产生分子量分别为151kDa、142kDa和117kDa的三种葡聚糖蔗糖酶,其中前两者同时存在于上清液中和细胞表面,而后者只存在于上清液当中;菌株DM1-2可以产生两种葡聚糖蔗糖酶,分子量分别为183kDa和142kDa,前者同时存在于上清液中和细胞表面,而后者仅能存在于细胞表面;菌株PC13可以产生分子量分别为148 kDa、138 kDa和115kDa的三种葡聚糖蔗糖酶,前两者属于胞外酶,而后者与细胞相连;与菌株6055相似,菌株L4可以产生两种同时存在于上清液和细胞表面的葡聚糖蔗糖酶,分子量分别为145 kDa和136kDa,而分子量为115kDa的葡聚糖蔗糖酶只存在于上清液中。

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子,在许多同种的真核生物有它的同源基因,这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关,而这个家族的蛋白质和DNA的结合能,各种ARID蛋白质的专一性尽相同,过大致上偏好於和AT-rich的序结合;我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因,分别是garid1和garid2,我们首先对於garid1做分析;将AU1标记接到gARID1转染形鞭毛虫,用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下,过其阳性染色和蛋白质表现有明显增加;EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合,经由突变序分析,也显示gARID1的结合序为AGATC和AATAAAATA,随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

Results: electronic microscope showed that in prednisone group heterochromatin increased in some cells, entoblast was not obvious in some cells, fat drops were small and few, mitochondria vacuole were common, and myelin figure was found in smooth endoplamic reticulum. the ultrastructure in yc group was almost normal and megamitochondrion formation was common. a large amount of fat drops were found in cytoplasm in gw group and little improvement was found compared with that in prednisone group.

结果: 电镜结果显示:激素组个别细胞异染色质增多,核仁不明显,脂滴显著变小变少,线粒体空泡化常见,有滑面内质网髓鞘样结构形成;养阴组电镜结构基本正常,其特征是细胞内有较多的巨大线粒体形成;桂附组较激素组改善不明显,其特征是胞质内可见大量脂滴。

The region between the circular brown band generated by cell lipid exosmosis and the band of the wound in the non-transgenic cotton Z35's leaves, is wider than that of the transgenic cotton T-34's leaves, and no micro-HR was found in Z35, the intensity of leaves wilting is much serious, by contrast, micro BR was found around the wound region within the transgenic cotton leaves inoculated with the pathogen, but the micro-HR was not found in the transgenic cotton treated with water and around wound region of non-transgenic cotton Z35 leaves which showed that there was micro-HR defence in transgenic hpa1xoo cotton.

棉花黄萎病菌侵染叶片后,非转基因棉花叶部伤口周围细胞无微过敏性反应,受病原菌侵染的叶片萎蔫程度较重;转hpa1xoo基因棉花叶部伤口周围细胞有微过敏反应,叶部刺伤处仅有较小的坏死斑,且不相连,发病较轻,而清水处理的转基因棉花与非转基因棉花叶片中伤口周围均无微过敏反应,说明病原菌侵染诱导转hpa1xoo基因棉花产生微过敏防御反应,转hpa1xoo基因棉花较非转基因棉花有较强的抗病性。

Results: Among 32 cases, abnormality of vertebral artery was found in 31 cases, abnormality of transverse foramen was found in 6 cases, hyperplasia of Luschka joint were found in 20 cases.

结果: 32例患者中有椎动脉异常表现者31例,横突孔异常者6例,钩椎关节增生者20例。

I scarce knew in what Manner to receive them; but found to draw our selves in a close Line was the only Way: so we form'd in a Moment: But that we might not have too much Interval, I order'd, that only every other Man should fire, and that the others who had not fir'd should stand ready to give them a second Volley immediately, if they continued to advance upon us, and that then those who had fir'd at first, should not pretend to load their Fusees again, but stand ready with every one a Pistol; for we were all arm'd with a Fusee, and a Pair of Pistols each Man; so we were by this Method able to fire six Volleys, half of us at a Time; however, at present we had no Necessity; for upon firing the first Volley, the Enemy made a full Stop, being terrify'd as well with the Noise, as with the Fire; four of them being shot into the Head, dropp'd, several others were wounded, and went bleeding off, as we could see by the Snow: I found they stopp'd, but did not immediately retreat; whereupon remembring that I had been told, that the fiercest Creatures were terrify'd at the Voice of a Man, I caus'd all our Company to hollow as loud as we could; and I found the Notion not altogether mistaken; for upon our Shout, they began to retire, and turn about; then I order'd a second Volley to be fir'd, in their Rear, which put them to the Gallop, and away they went to the Woods.

我简直不知道如何对付它们。结果,我认为最好的办法是我们互相靠拢,排成一行。于是,我们马上照此行事。为了不致使我们的火力中断太久,我下令只许一半人开枪,另一半人作好准备;如果第一排枪响过后,狼群继续向我们冲来,就开第二排枪;同时,在开第二排枪时,那开第一排枪的一半人,不要忙于装他们的长枪,而是应该抽出手枪,作好准备。因为我们每人身上都有一支长枪和两支手枪。用这种办法,我们可以连续开六排枪,每次有一半人开枪。然而,当时还没有必要这样做。放出第一排枪之后,我们的敌人就给枪声和火光吓坏了,马上停止了前进。有四条狼被我们打中头部,倒了下来;另外有几条受了伤,鲜血淋淋地跑掉了。这在雪地上可以看得一清二楚。我发现,狼群停止了攻击,但没有后退。这时,我忽然记起有人说过,就是最凶猛的野兽,听见人的声音也会害怕。于是我就叫大家拼命呐喊。这个办法果然很有效。我们一喊,狼群就开始后退,掉头跑掉了。我又下令朝它们背后开了一排枪。这样一来,它们才撒腿跑回树林里去了。这时,我们才有时间重新给枪装上弹药。

Results K14 were found in the early, middle and late human fetal epidermis. But highest level was found in the middle gestion. K14 was also found in higher level in the late gestation but mainly localized in the basal layer.

结果 人胚胎12周表皮已表达K14,中期表达量最高,晚期也有较高表达,但主要局限于表皮基底层细胞。

Minerals found in the region has 31 major categories, representing the province's mineral resources have been found in 23% and found that more than 160 types of mineral point, the proved reserves of 15 kinds of minerals, of which: ilmenite, graphite ore are domestic large mineral reserves of 6,000,000 tons and 4,000,000 tons or more; limestone, gypsum, Glauber's salt, granite and coal and other non-metallic fuel and energy resources in the northwest edge itself, which proved coal reserves of 1.068 billion tons, oil-page rock 248 million tons, 7.73 million tons of Glauber's salt, 1.17 billion tons of limestone, gypsum 140 million tons, due to a low degree of geological exploration, the comprehensive development of a low utilization rate, at present the development and utilization of mineral ores accounted for only 30% of the region, cooperating with the outside great potential for development.

全区已发现矿产9大类31种,约占全省已发现矿产的23%,发现各类矿产点160余处,探明储量的矿种15种,其中:钛铁矿、石墨矿属国内特大型矿产,储量分别为600万吨和400万吨以上;石灰石、石膏、芒硝、花岗岩和煤炭等非金属和能源燃料类资源在西北占居优势,其中煤炭探明储量10.68亿吨、油页岩2.48亿吨、芒硝773万吨、石灰岩11.7亿吨、石膏1.4亿吨,由于地质勘探程度低,综合开发利用率低,目前开发利用的矿种仅占全区矿种的30%,对外合作开发潜力巨大。

Results Among 54 WD patients, 36 were found to carry Arg778leu mutation, among whom 15 were homozygote, 21 were heterozygote, the frequency of this mutation was 47.2%. Of 46 patients with hepatic damage, 36 were found to have the mutation of Arg778Leu, among whom 34 were found to have Leu770Leu polymorphism simultaneously.

结果 54例Wilson病患者中36例存在Arg778Leu基因突变,15例为纯合型,21例为杂合型,Arg778Leu等位基因突变频率为47.2 %;46例伴肝损害的患者中33例存在ATP7B基因外显子8的Arg778Leu突变;36例Arg778Leu突变者中34例同时伴有Leu770Leu多态性。

After analyzed the result of experimentation, I found out the mathematics model between the speed of liquid and the variable of resistance.according to the minus- resistance characteristic, I brought forward a new way of hydrometry, found corresponding mathematics model of hydrometry, designed a metrical system and brought forward some advice of about this system, found some significative conclusion.

分析实验数据,验证了液体流速与其电阻变化量之间的数学模型。依据负阻特性,提出了一种新的流量测量方法,建立了相应的流量测量数学模型,并设计了一套对应的测量系统,提出了该系统的设计要点,得到了一些有意义的结论。

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推荐网络例句

And he is one of the few scouts who are occasionally sent out to have dealings with other peoples.

而他也是少数的几位偶尔会被派出去和外界人打交道的巡逻人员之一。

The sons of Hebron: Korah, Tappuah, Rekem and Shema.

希伯仑的儿子是可拉,他普亚,利肯,示玛。

He will take part in an important race across the Atlantic.

他将参加一次重大的横渡大西洋的比赛。