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Under normal temperature.the Ca2+-ATPase activity was found on the innerside of the PM.on the vacuole envelope and the chloroplast envelope,and theenzyme activity declined upon heat stress.The Ca2+-ATPase activity on the vacuoleenvelope,PM.and the chloroplast envelope in both Ca2+-and oxalate-treated peppermesophyll cells was higher than that in the control under the same condition.TheCa2+-ATPase was also found at the granum lamella,and it was activatedsignificantly by exogenous Ca2+ and oxalate treatments.Under heat stress.the Ca2+-ATPase activity declined slowly in both Ca2+- and oxalate-treated mesophyll cells.But La3+ treatment inhibited the enzyme activity under both normal condition andheat stress.

常温下生长的叶肉细胞,在质膜、液泡膜、叶绿体被膜等处有Ca2+-ATPase活性,热胁迫后酶活性下降;外源Ca2+和草酸预处理对辣椒叶肉细胞各种膜上Ca2+-ATPase活性具有促进作用,特别是定位于液泡膜、质膜和叶绿体被膜上的酶活性明显比对照提高;在叶绿体基粒和基质片层上也有酶活性,并且Ca2+和草酸预处理对该部位上的酶活性激活作用更明显;La3+处理的作用与Ca2+和草酸处理的效果相反。

When No. 3 oil is used, put in 3000rpm, 8. 59Nm, the gear surface has an obvious plastic flow, an obvious groove is found on pitch line of the driving gear , an obvious edge is found on pitch line of the driven gear . The operating surfaces of both driving gear and the driven one is high in smooth finish, no pitting. Through the analysis on the gear surface only based on the gear in experiment with No.

实验的结果为:ISOVG68机械油作润滑油时,在输入3000rpm,5.96Nm时,齿面发现沿节线出现大面积的点蚀,且齿面有塑性变形;以3#油为润滑油时,在输入3000rpm,8.59Nm时,齿面发生了明显的齿面塑性流动,主动轮在节线处出现明显的沟槽,从动轮在节线处出现明显的棱脊,大小齿轮工作齿面均十分光洁,未发现点蚀现象发生。

The anastomosing occurred between the pleural superficial and the subpleural vascular networks on the capillary level or the precapillary level. The honeycomb vascular networks were also various in size and form at a low magnification. The venule connected with the pulmonary capillary networks directly at a high magnification. The single capillary networks were found in the alveolar septa. The longitudinal crests and dense round imprints of endothelial nuclei and the clear circular constrictions were found on the surface of the larger vessel in the lung parenchyma. The round imprints of endothelial nuclei also occurred on the subpleural venule and capillary.

胸膜面浅层的微血管与胸膜下微血管之间形成毛细血管水平或毛细血管前水平的吻合;低倍镜下肺实质内微血管网也呈现大小不等、形态各异的蜂窝状结构;高倍镜下肺泡毛细血管与微静脉之间联系紧密;在肺泡隔内毛细血管网成单层分布;肺实质内管径较大的微血管表面可以见到纵行的嵴状结构和密集的内皮细胞核的压痕,以及明显的环形缩窄;胸膜下微静脉和毛细血管表面可见圆形内皮细胞核的压痕。

It is shown that nanoscale pyramid cone shapes are found on the metal film interface (100), serrate steps are found on the metal film interface (111), and cystiform shapes with terrace structure are found on the cross-section near the catalyst. Other faults have brittleness fracture characteristic with nanoscale clusters are found on the fault surface.

结果表明:包膜(100)界面有纳米级的棱锥状突起,包膜(111)界面上分布着锯齿状的台阶;金属包膜近触媒侧的界面断面表层分布着台阶形貌的胞状突起;断面其它断层断口呈现脆性断裂特征,断层表面分布着团簇状的纳米颗粒。

Results The negative correlations were found on the Glasgow Outcome Scoreto the items such as conscious state at admission,Glasgow Coma Score,psychiatric symptoms,hemiplegia,Babinski sign,abnomal degree of Electroencephalogram,focal lesions found by neuro-image investigation and mean peak flow velocityand pulsatility indexof basilar artery and vertebral arteries by transcranial Doppler ultrasonography investigation;The non-correlation was found on the Glasgow Outcome Score to theitems such as headache,febrile,epilepˉsia style,the involvement of cranial nerve,dystaxia,neck stiffness,Cerebrospinal fluid investigation and blood serum enzymology.

结果 入院时的意识状态、GCS、精神症状、偏瘫和/或偏身感觉障碍、巴彬斯基征以及脑电图异常程度、影像学所见的病灶范围、基底动脉和椎动脉的平均峰流速、脉动指数和Glasgow预后评分呈负相关;头痛、发热、癫痫类型、颅神经受累情况、共济运动、脑膜刺激征、脑脊液检查(含压力、蛋白定量、细胞数、HSV-PCR)以及血清酶学的变化和Glasgow预后评分之间无相关性。结论入院时的意识状态、GCS、精神症状、局灶性神经系统体征以及脑电图异常程度、影像学所见的病灶范围、后循环的血管痉挛程度和血流阻力等是影响病脑预后的主要因素。

Results The negative correlations were found on the Glasgow Outcome Scoreto the items such as conscious state at admission,Glasgow Coma Score,psychiatric symptoms,hemiplegia,Babinski sign,abnomal degree of Electroencephalogram,focal lesions found by neuro-image investigation and mean peak flow velocityand pulsatility indexof basilar artery and vertebral arteries by transcranial Doppler ultrasonography investigation;The non-correlation was found on the Glasgow Outcome Score to theitems such as headache,febrile,epilepˉsia style,the involvement of cranial nerve,dystaxia,neck stiffness,Cerebrospinal fluid investigation and blood serum enzymology.

结果 入院时的意识状态、GCS、精神症状、偏瘫和/或偏身感觉障碍、巴彬斯基征以及脑电图异常程度、影像学所见的病灶范围、基底动脉和椎动脉的平均峰流速、脉动指数与Glasgow预后评分呈负相关;头痛、发热、癫痫类型、颅神经受累情况、共济运动、脑膜刺激征、脑脊液检查(含压力、蛋白定量、细胞数、HSV-PCR)以及血清酶学的变化与Glasgow预后评分之间无相关性。结论入院时的意识状态、GCS、精神症状、局灶性神经系统体征以及脑电图异常程度、影像学所见的病灶范围、后循环的血管痉挛程度和血流阻力等是影响病脑预后的主要因素。

Results: NKA-I cells was first found in the duodenum mucose on the 14th day of fetus, earlier than in the jejunum ( 16th day ) and ileum ( 17th day ), and its Positive reaction and the number of cells increased with further development, after birth the 30th day, it became quite identical to that of adult rat; NKA-I cells were seen in the colon mucosa on the 17th day of fetus, later on the number kept decreasing till grown up stage; No change was observed in the stomach mucosa except that few cells showed up on the 15th day of fetus; No positive cell was found on the oesphagus wall.

结果: NKA-I细胞首先见于胚胎14 d的十二指肠粘膜,早于空肠(胚胎16 d)和回肠(胚胎17 d),其阳性反应强度及细胞量随发育而增加,30 d时与成年鼠相似;结肠粘膜内于胚胎17 d见细胞,但此后数量较少直至成年;胃粘膜于胚胎15 d见个别细胞外,以后无明显变化;食道壁始终未见阳性细胞。

Results: NKA-I cells was first found in the duodenum mucose on the 14th day of fetus, earlier than in the jejunum ( 16th day ) and ileum ( 17th day ), and its Positive reaction and the number of cells increased with further development, after birth the 30th day, it became quite identical to that of adult rat; NKA-I cells were seen in the colon mucosa on the 17th day of fetus, later on the number kept decreasing till grown up stage; No change was observed in the stomach mucosa except that few cells showed up on the 15th day of fetus; No positive cell was found on the oesphagus wall.

结果:NKA-I细胞首先见于胚胎14 d的十二指肠粘膜,早于空肠(胚胎16 d)和回肠(胚胎17 d),其阳性反应强度及细胞数量随发育而增加,30 d时与成年鼠相似;结肠粘膜内于胚胎17 d见多个细胞,但此后数量较少直至成年;胃粘膜于胚胎15 d见个别细胞外,以后无明显变化;食道壁始终未见阳性细胞。

The most common potential pathogen, coagulase-negative staphylococci, were found on all of the keyboards. Diphtheroids were found on 80% of keyboards, Micrococcus species on 72%, Bacillus species on 64%, nonfermentative Gram-negative rods on 36%, vancomycin-sensitive Enterococcus on 12%, Staphylococcus aureus on 4%, and oxacillin-susceptible S aureus on 4%.

在所有键盘中都发现了最常见的致病菌凝固酶阴性葡萄球菌。80%的键盘带有类白喉菌,72%带有微球菌属,64%带有芽胞杆菌属,36%带有非发酵的革兰氏阴性杆菌,12%带有万古霉素敏感肠球菌,4%带有金黄色葡萄球菌,4%带有甲苯异恶唑青霉素易感链球菌。

Therefore we have optimized the experimental system and the working status of instrument.2 The near-field SERS spectra and images for the [Ru_3]~(2+) molecule adsorbed on silver nanoparticle substrate were achieved. Based on the results, some specific phenomena for near-field SERS were found. By observing the near-field SERS spectra collected at different location at the nano-scale, the different spectral characteristics which are caused by the local environmental differences of molecules have been found. On the substrate, the highly SERS active sites are much more than SEF hot spots, and the distribution of them is not entirely consistent. This phenomenon could be explained by the influences of several factors including the different enhancement efficiencies existed for SERS and SEF, the quenching effects for fluorescence by the metal substrate and metal-coated tip, and the presence of different numbers of probing molecules in SERS and SEF.3 It is of interest that the near-field and far-field SER spectra demonstrate several distinctively different spectral features.

由于SNOM与SERS的结合在技术上将面临很多实际困难,例如近场激发信号极其微弱、纳米级微弱光谱的收集对实验精度和环境稳定性的苛刻要求等,因此我们对实验体系和仪器工作状态分别进行了优化。2获得了银纳米粒子SERS基底上[Ru_3]~(2+)分子在纳米尺度下的近场SERS光谱和图像,从中可以发现一些近场SERS效应特有的现象:通过观察不同位置得到的近场SERS光谱可以发现,在纳米尺度下,由于分子所处局域环境不同而体现出不同的光谱特征;此基底具有极高SERS活性的位点远多于表面增强荧光热点,且两者的分布并不完全一致。

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