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Was simultaneously injected into cisterna magna at the second injection blood in the presence of subarachnoid blood. At 2 to 4 days, GFP was expressed in leptomeninges over the brain stem, cortex and cerebral arteries, smooth muscle cells of small vessels were occasionlly transduced. GFP was expressed in adventitia of spastic basilar artery on days 2 (day 5 after first injection blood) after injection 〓, but was undetectable by days 4, transgene was not expressed in medial or intimal layers. The prensence of subarachnoid blood can not prevent access of adenovirus to vessels and transgene expression.

注入腺病毒载体后2天（初次注血第5天）行荧光显微镜、免疫组织化学和RT-PCR检测，结果在颅内大血管如基底动脉的外膜可见外源基因的表达，而外源基因不能转移至血管中膜和内膜，4天时（初次注血第7天）基底动脉的外膜中外源基因表达消失；注入腺病毒载体后2～4天，外源基因可以有效转移至颅底软脑膜细胞，小血管外膜和平滑肌层也可见外源基因的表达，表明蛛网膜下腔内的血凝块不能阻止腺病毒载体介导外源基因转移至颅内血管。

INTERVENTION: The models of ischemia/reperfusion in SD rats were established by middle cerebral artery occlusion with a nylon monofilament suture. The rats were randomly divided into the treatment groups 32cases and the control group 32 cases. Each group was divided into eight subgroups, which consisted of 4 rats at 2 hours, 6 hours, 12 hours, 24 hours, 2days , 3 days, 7 days and 14 days after reperfusion of MACO.

干预措施：成年健康雌性SD大鼠68只，应用线栓法建立SD大鼠大脑中动脉阻塞再灌注模型，随机分为治疗组32只和对照组32只，每组再随机分为缺血1.5 h再灌流2，6，12，24 h，2，3，7，14 d组(n=4)，另外4只作假手术组。

No season is superior to another season for this sweetest of all acts. God never made salvation depend upon new moons nor holy days or sabbaths. A man is not nearer to Christ on Easter Sunday than he is, say, on Saturday, August 3, or Monday, October 4. As long as Christ sits on the mediatorial throne every day is a good day and all days are days of salvation.

只要你把心舉起來，讓它安息在耶穌里，即刻就進入至圣所，無論你所在的地方是一個頂舒服的車廂，或是一間工厂，或是一個廚房，只要你的思想是向著神，并且順服人，你就能在那個地方遇見神。

The result showed that the development of Microtus brandti could be divided into 3 phases according to the content of mitochondrial protein in liver: 1-3 days, 4-19 days, 20 days to adult.

结果发现根据幼鼠肝脏内线粒体蛋白的含量可将生长发育大致分为3个阶段：1～3日龄，4～19日龄，20日龄至成体。

Result show that TRAP-positive mononucleated and multinucleated cells were markedly induced at the compression side at 3, 5, and 7 days. Mononucleated osteoclast reached peak level at 3 days after tooth movement, while multinucleated cells reached peak level at 5 days.

破骨细胞酶组化标记和计数统计显示，TRAP阳性细胞数量在牙移动第3、5和7天时与对照组比较有显著性差异；其中单核破骨细胞在第3天、多核破骨细胞在第5天时达到峰值。

As for the Nepionic Rats at the body growth characteristic of different days ages,the method of observing the distance and the relative distance of the anus and the vulvas when they were three to four days old was used to discriminate the sex.When they are five to eight days old,the method of observing the papillas was added to discriminate the sex.

将同期交配的孕鼠分为4组，针对乳鼠在不同日龄的身体发育特点，在3～4日龄采用观察其肛门与外阴距离和相对距离的方法、对5～8日龄再附加对乳头观察的方法进行性别鉴别对比实验。

Methods The pregnant rats mating in the corresponding period were divided into four groups.As for the Nepionic Rats at the body growth characteristic of different days ages,the method of observing the distance and the relative distance of the anus and the vulvas when they were three to four days old was used to discriminate the sex.When they are five to eight days old,the method of observing the papillas was added to discriminate the sex.

将同期交配的孕鼠分为4组，针对乳鼠在不同日龄的身体发育特点，在3～4日龄采用观察其肛门与外阴距离和相对距离的方法、对5～8日龄再附加对乳头观察的方法进行性别鉴别对比实验。

The outer neuroblastic layer before it divided to inner and outer nuclear layer and outer complexiform layer and gonglion cell layer showed a temporal PCNA positive which was related to proliferation. The three cell layers had another PCNA positive when finished time changed from outer to inner after their morphologic development basically completed( outer nuclear layer:11～15 days,inner nuclear layer: 8～16 days, gonglion cell layer:16～21 days).

PCNA显示在外成神经细胞层分化为内、外核层和外丛状层之前，和节细胞各有一次和增殖相关的阳性表达。3个细胞层在形态学的分化基本完成后，又出现了一次结束时间是由外向内变化的阳性过程（外核层：第11天至15天：内核层：第8天至16天，神经节细胞层： 16天到21天，这一次阳性表达过程可见到细胞形态向成年形态的转化。

Methods Seventy six aged patients with ostalgia caused by primary osteoporosis, whose T numerus less than certified value 2.5 were randomized into two groups: 1. Applying only CT (36 cases); 2. CT combined with Low frequency transduction pulsed electromagnetic fields (36 cases); Two groups simultaneously were given Calcium 600 mg and injected CT 50IU, ten days later injected 50IU every two days in two months, Low frequency transduction pulsed electromagnetic fields treatment was made by Tianjin Jinxitong company, frequency 20-40Hz, and treated 20 minutes by this machine, totally 30 days. Six months later we examined their Bone density again.

将存在骨质疏松性骨痛同时经DEXA骨密度仪检测T值低于标准值2.0以下的老龄原发性骨质疏松症患者76人随机分为两组：①单纯应用鲑鱼降钙素组（36人）；②联合应用药物及低频电磁场组（36人），两组同时每日给予钙而奇D600片1粒，CT按每日肌肉注射50IU，10d后改为隔日肌注50IU连续应用2个月，低频电磁场治疗采用天津金希统公司生产的XT2000B型OP治疗仪，频率为20~40Hz，每天治疗20min，共治疗30d， 6个月后复查骨密度。

The polyspermy rate was significantly decreased when the semen samples were stored for longer (3 days). Some cleavage embryos were collected to identify their sexes in 3-4 days after in vitro fertilization. The ratio of male embryos, produced from the BTS semens stored for 1 and 3 days, were 60 % and 41 %, respectively, and 35 % and 48 % in the MIII semens.

使用不同稀释液，保存1与3日之精液进行体外受精，在培养3 - 4日后收集卵裂胚进行性别鉴定，在保存1日与3日之稀释液BTS组，雄胚性比率分别为60 %与41 %；在稀释液MIII组，保存1日与3日之雄性比率分别为35 %与48 %。

More direct, directer ; most direct, directest

径直的，笔直的

Do you know how to use a chain saw?

你知道如何使用链锯吗？