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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法,研究丘脑网状核(nucleus reticularis thalami,RT)中γ-氨基丁酸(gamma-amino butyric acid ,GABA)能神经元、一氧化氮(nitrogen monoxidum,NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate,cAMP)及中缝背核(nucleus raphes dorsalis,DRN)至RT的5-羟色胺(5-hydroxytryptamine,5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP,5μg),注射当天睡眠时间略有减少,第二日睡眠时间显著减少,觉醒时间明显增多,第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后,与生理盐水组比较,睡眠时间增加,觉醒时间减少;而RT内微量注射L-谷氨酸(glutamic acid, L-Glu, 0.2μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline,BIC,1.0μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen,BAC,1.0μg)后,产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg,0.5μg)后,与生理盐水组对比,睡眠时间略有减少,但无显著性意义;而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside,SNP,0.2μg)后可明显增加觉醒时间,缩短睡眠时间;微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine,L-NNA,2.0μg)后,引起睡眠时间增多,觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用;RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠,其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate,MOR,1.0μg)后与生理盐水组对比,睡眠时间减少而觉醒时间增加; RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride,NAL,1.0μg)后与生理盐水组比较,睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后,与生理盐水组对比,原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较,睡眠时间增多而觉醒时间减少;RT内微量注射亚甲蓝(methylene blue,MB,1.0μg)后,与NS组比较,睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 0.2μg)比较,睡眠时间减少,觉醒时间增多;大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射二甲基麦角新碱(methysergide, MS, 1.0μg )后,与对照组(DRN注射L-Glu 0.2μg,双侧RT注射NS 1.0μg)比较,睡眠时间增多,觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA,10μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 1.0μg)比较,睡眠时间增多,觉醒时间减少;大鼠DRN内微量注射PCPA(10μg),产生睡眠增多效应后,在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan , 5-HTP, 1.0μg )后,与对照组(DRN注射PCPA 10μg,双侧RT注射NS 1.0μg)比较,睡眠时间减少,觉醒时间增多。

So say American film society to sue fast thunder can additional purposeful, these year western country always censures China in intellectual property domain, because,force of fire of before a few years of Zhuhai is sued by SIGMATEL of provider of American MP3 chip also is intellectual property, the swift and violent development that real reason is Zhuhai fire force truly has browbeaten SIGMATEL, the intellectual property case that SIGMATEL prosecutes Zhuhai fire force also is absent MP3, and in domain of power source power comsumption, allegedly the period of time of to lodge a complaint, the client of force of many Zhuhai fire converts SIGMATEL, because,very simple everybody does not consider the matter the lawsuit ask for trouble of Zhuhai fire force, this just also is the real reason that SIGMATEL sues Zhuhai fire force, do sth over and over again prosecution of two years is Zhuhai fire force as a result eventually most reconcile in both sides of Shenzhen countercharge SIGMATEL, the judgement that the market share of force of final Zhuhai fire exceeds SIGMATEL to became the MP3 chip provider with the greatest whole world to also prove SIGMATEL is original and be anxious.

这么说来美国电影协会起诉迅雷只能另有目的,这些年西方国家总是在知识产权领域指责中国,前几年珠海炬力被美国MP3芯片提供商SIGMATEL起诉也是因为知识产权,真正的原因确是珠海炬力的迅猛发展已经威胁到SIGMATEL,SIGMATEL起诉珠海炬力的知识产权案也不在MP3,而在电源功耗领域,据说提起诉讼的一段时间,大量珠海炬力的客户都转投SIGMATEL,原因很简单大家都不想因为珠海炬力的官司惹火上身,这也才是SIGMATEL起诉珠海炬力的真正原因,折腾了两年的起诉最终结果是珠海炬力在深圳反诉SIGMATEL双方和解,最后珠海炬力的市场份额超过SIGMATEL成为全球最大的MP3芯片提供商也证实了SIGMATEL最初的判定和担忧。

The paper consists of following contents:(1)starting with a historicalreview of the use-of-force,this paper reveals a paradoxical evolvementof the two trends in use-of-force,compares the absolutization andinfinity with controllability and finity of the use-of-force,and clarifiesnew characteristics of the use-of-force in post-Cold War era;(2)bysystematically analyzing the era background from the viewpoints ofeconomic globalization,political multipole,military high tech,national separatism and the hegemonism,the paper clarifies factorslimiting and stimulating the use-of-force;(3)by analyzing the lawbackground mainly from the viewpoint of international laws,the paperespecially centralizes the discussion on restrictions made by moderninternational laws on the use-of-force and their limits,dissects thechallenges brought up by the high tech war to international laws;(4)the use-of-force by UN and other regional international organization;(5)refuting the theory of"humanitarian intervention"advocated bythe USA and certain western countries,the paper expatiates the rightrelations between human rights and sovereignty,and demonstrates therightness of the sovereignties and UN;(6)the basic guidelines of theuse-of-force in the post-Cold War era.

分别从经济全球化、政治多极化、军事高技术化以及民族分离主义的泛滥、霸权主义的恶性膨胀等方面系统分析冷战后武力使用的时代背景,揭示出冷战后时代同时存在的限制、约束武力使用的因素,以及诱发、刺激武力使用的因素。(3)主要从国际法的角度分析武力使用的法律背景,着重探讨现代国际法对武力使用的规范和制约作用以及这种作用的有限性,同时剖析现代高技术战争对国际法提出的挑战。(4)主要探讨联合国以及区域性国际组织在冷战后使用武力的实践以及存在的问题。(5)驳斥以美国为首的某些西方国家所鼓吹的&人道主义干涉&论,阐述人权与主权的正确关系,论证主权国家和联合国并没有过时,不能用非人道手段解决人道主义问题。(6)系统提出了冷战后国家间使用武力应遵守的基本准则。

We want to move up ticklish Stone, the facilities of the moment will be greater than the force of gravity produced by a rock moment, we all know that the moment arm of force multiplied by the force equal to the size of the arm of force as an arm of force in the greater 2 , Finger pressure, the more gravity than, stone ticklish ticklish point of this very long arm of force, so we used a very small force so that the stones will be able to move up.

我们要想让怕痒石动起来,所施的力的力矩就要大于石头重力所产生的力矩,我们都知道力矩等于力臂乘以力的大小,当力臂2越大于力臂1时,手指压力也就越小于重力,怕痒石怕痒的这个点的力臂非常长,所以我们用很小的力就能使石头动起来。

The force is dominated by entropy force when the chain isdeformed less than 0.9 of the full-extended chain, while by enthalpy force. Theenthalpy force is contributed by the intro-molecular interaction. Bond stretchcontributes to the force more than bond angle bend. Torsion and VDW contributelittle to the force.3. The internal energy contribution to the elastic force It is in dispute that whether 〓 is dependent on the strain, and it is hard toresolve the problem using present elastic theories because they are athermal.

我们模拟了聚乙烯模型聚合物的SMFS,研究了链长、势能有效距离和温度对力谱的影响,探讨了力谱的本质问题,得到以下结果:改进的分子动力学方法可以很好的模拟聚合物的SMFS;SMFS是链段的性质而不是整个高分子链的性质,对于聚乙烯,该链段碳原子数目大于40;SMFS的本质是熵弹性,形变小于全伸直链的0.9时,力为熵弹力;形变超过全伸直链的0.9时,能弹力占主导地位,这种能弹力完全是分子内相互作用引起的,是由键伸缩和键角弯曲贡献的。3。

After the analyses of the force about different shape of caterpillar thorn, it was considered that the drive force of caterpillar tractor consisted of friction force, extruding and cutting force, edge cutting force and thorn friction force.

一般认为,拖拉机在田间行驶时,切线牵弓l力是由土壤抗剪力产生的,其计算公式通过土壤剪切力导出11·2}。这一观点忽略了其它一些因素的影响,并且只适用于直板式履刺。

The generalized force consists of the generalized conservative force, the generalized dissipation force and the generalized force in correspondence with the generalized coordinate except the generalized conservative force and the generalized dissipation force.

基于非自治有阻尼系统推导了第二类Lagrange方程,使用虚功原理,给出了广义力的求解方法,广义力由广义保守力、广义耗散力、除广义保守力和广义耗散力以外的对应于广义坐标的广义力这3部分组成。

In this paper the conflicting discussion of scholastic view-point on seepage force which has lasted many years is stated. Then the physical concept of seepage force and its related forces including the resolved two components (a seepage force and a buoyant force) from seepage resultant force, the transformation between the seepage force and boundary pore water pressures, schematic diagram of forces acting on unit soil mass and the mathematic proof by Green theorem are described.

追述了多年来对渗透力学术观点的争论,并再就此问题论述了渗透力的物理概念及其与之相关的各力(包括渗流作用合力及其分解成的两个分力,渗透力与周边孔隙水压力的转换关系及各力作用在单位土体的图示;从数学上引用格林公式,证明土体表面水压力与土体本身渗透力之间的转换关系。

Through the analysis, the paper reaches the following basic conclusions: The quantity of Chaoyang district"s transient population will increase continuously, residential period will prolong gradually and renting house will still be the major residential way of transient population; transient population play a positive role in the district"s socioeconomic development, but on the other side they make city infrastructures more difficult to bear the existent heavy burden, difficulty in city management is increased; transient labor force mainly undertake the supplementary role of the district labor force, but along with the open of the labor force market step by step and the further improving of the quality of transient labor force, the competition between transient labor force and local labor force tends to be intense; the current management policy of transient population in Beijing exists some problems, which stresses management and makes light of service, stresses duties and makes light of rights and interests, stresses planning and makes light of market, stresses outside and makes light of inside, but the policy is tending to the developing direction that is helpful for transient population to flow.

文章通过分析得出以下基本结论:朝阳区外来人口的数量还会继续增长,居住时间逐渐延长,租住房屋仍然是外来人口最主要的居住方式;外来人口在为本区社会经济发展起到积极作用的同时,也使城市基础设施不堪重负,城市管理难度加大;外来劳动力主要承担着本区劳动力的补充角色,但随着劳动力市场的逐步开放和外来人口素质的进一步提高,外来劳动力与本地劳动力的竞争趋于激烈;北京市现行的外来人口管理政策存在着重管理而轻服务、重义务而轻权益、重计划而轻市场、重外部而轻内部的问题,但正趋于有利于外来人口流动的方向发展。

The results of calculations showed that the maximum increments in saltation height and horizontal distance due to the Magnus force were 10.2 and 24.9%, respectively, and that both increments increased with increasing lift-off angle. The maximum increments in saltation height and horizontal distance of sand grains caused by the Saffman force were only 4.6 and 3.7%, respectively. The vertical concentration distribution of Aeolian sand flow follows exponential function distribution whether or not force analysis of saltating sand grains includes Magnus and Saffman force. The effect of Magnus and Saffman force on the vertical concentration distribution of Aeolian sand current makes the rate decrease near sand bed and it is consistent with the effects of Magnus and Saffman force on the saltation height and distance of sand grains.

Magnus力对跃移高度和水平距离产生的最大增量分别可达到10.2%和24.9%,这两个增量均随起跃角度的增加而增大;Saffman力对跃移高度和水平跃移距离产生的最大增量分别为4.6%和3.7%;是否考虑Magnus与Saffman力,风沙流垂向质量百分浓度均服从指数函数分布,Magnus与Saffman力使得风沙流垂向质量百分浓度较不考虑它们的作用呈现出底层的比例较小,上层较大,Magnus与Saffman力对跃移高度的影响基本与其对风沙流垂向质量分布的影响一致,不过Magus力对风沙流垂向质量百分浓度分布影响较大,0-2cm范围内尤为强烈(7.16-10.81),其他高度层(2-20 cm)的影响相对较小(0-2.95)。

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The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他,我不喜欢他的这种性格。

As we know the price of traditional product is composed of the cost and the profit of the company involving market competition, monopolizes and many other factors.

我们知道作为传统的商品,定价的模式往往是在成本的基础上增加厂商的预计利润而形成其价格,当然也要考虑到市场竞争、垄断等其他方面的因素。