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We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

For female chickens, however, the body weight at 14 wks of age for AA was greater (p.05) than that for TT. Therefore, P0U1F1_6 is an effective molecular marker that can be used in MAS program for early growth rate in chicken, especially for male chickens.The better understanding of the mechanism of broodiness stands great significance not only for avian genetics, behavior, and endocrinology, but also for the acceleration of poultry breeding progress against broodiness to enhance egg producti

研究家禽就巢性的遗传机理，不但对鸟类遗传学、行为学、内分泌学等方面有重要的理论意义，而且本研究成果有助于加速无就巢性或低就巢性的家禽品种或品系的培育，从而提高禽群的产蛋性能和种刚性能，尤其对我国地方禽种资源的开发利用有重要的意义，具有广阔的应用前景。

After 24 h reperfusion, these values were 403, 155 and 293 for PO2/FiO2; 3.7, 11.0 and 6.7 for lung injury pathology score; 3.7, 6.4 and 5.2 for wet-to-dry ratio; 2,3, 7.2, 5.0 for MPO activity; 0.7, 1.5, 1.2 for MDA activity in lung tissue, 2.5, 5.5, 4.6 for MDA in serum, 29.3, 43.1, 32.1 for CINC activity.

再灌注24 h，各组PO2/FiO2分别为403， 155，293；肺损伤病理评分为3.7， 11.0， 6.7；W/D分别为3.7， 6.4， 5.2；MPO活性分别为2.3， 7.2， 5.0 U·g-1；MDA肺组织中活性为0.7， 1.5， 1.2 nmol·mg pro-1；血清中活性为2.5， 5.5， 4.6 nmol·mL-1；CINC活性分别为29.3， 43.1， 32.1 pg·mg pro-1。

Results:The values were 388, 179 and 265 for PO2/FiO2; 3.2, 6.5 and 5.0 for lung injury pathology score; 3.5, 5.6 and 5.2 for wet-to-dry ratio; 2.3, 5.2, 4.4 for MPO activity, 0.8, 2.6, 1.6 for MDA activity in lung tissue, 2.3, 8.1, 5.2 for MDA in serum, 26.4, 114.4, 19.9 for CINC activity (Sham, control, and SS groups, respectively) at 2 h reperfusion.

结果：再灌注2 h，假手术组、对照组、西维来司钠组，PO2/FiO2 分别为388， 179， 265；肺损伤病理评分为3.2， 6.5， 5.0；W/D分别为3.5， 5.6， 5.2；MPO活性分别为2.3， 5.2， 4.4 U·g-1；MDA肺组织中活性为0.8， 2.6 1.6，血清中活性为2.3， 8.1， 5.2 nmol·mL-1；CINC活性分别为26.4， 114.4， 19.9 pg·mg pro-1。

This study is to give a solution for the estimation of flash point for mixtures, the necessity for classification of flammable liquids. The traditional models for predicting flash point of mixtures usually by the activity coefficient approach. However, the parameters of activity coefficient were regressed from phase equilibrium data in the literatures. If there is no such parameter in literatures for the desired mixture, the model cannot predict the flash point oft hat mixture. Thus, this research aims at the improvement of deficiency of the flash point prediction models in the literatures by useing UNIFAC (Universal Quasi-chemical Functional Group Activity Coefficient) equation, Dortmund-UNIFAC equation and Lyngby-UNIFAC equation. In this study, we were aim at the prediction model for miscible mixtures.From the result, it is suggested to use different type of UNIFAC equation to estimast activivity coefficient in the predition of flash point for different mixture type.

易燃液体分类的主要依据参数为闪火点值，因此本研究目的为解决易燃液体中混合物分类时所面临到的闪火点值缺乏的问题，而在过去文献中所提出的闪火点预测模式会使用到活性系数，但这些模式使用到的活性系数均需要相平衡的数据以回归其所需参数，若所欲估算闪火点的混合溶液文献上未有相关参数，则无法有效利用闪火点预测模式估算其闪火点，因此针对文献中现有闪火点预测模式的缺点，利用描述液体混合物活性系数UNIFAC (Universal Quasi-chemical Functional Group Activity Coefficient)方程式、Dortmund-UNIFAC方程式和 Lyngby-UNIFAC方程式，建立发展一闪火点预测模式，而本研究以互溶溶液为对象。

His main works are "Approach to Analyze Technology","Introduction to Technological Sociolization","Introduction to Philosophy of Social Technology", etc., and has published over hundreds of paper in journals of "Philosophy Study","Study in Dialectics of Nature" and other core periodicals and was also editor-in-chief for books of "Researches in Technology and Philosophy","Researches in Science Technology and Society", He is now with a part-time position of being a member of the Evaluation Panel for Discipline of Philosophy of the Academic Degree Committee of the State Council, Vice President of The Chinese Society for Dialectics of Nature, chairman of China Professional Committee for Philosophy of Technology, committeeman of China Professional Committee for Science, Technology and Society, chairman of the Association of Philosophy of Liaoning Province, Standing Deputy President of the Society for Dialectics of Nature of Liaoning Province, committeeman of Consultative committee of Liaoning Provincial Government and Shenyang Municipal Government, Scientific and Technical Consultant of Shenyang Municipal Government, member of the International Society for Philosophy of Technology.

主要论著有：《解析技术》，《技术社会化引论》，《社会技术哲学引论》等，并在《哲学研究》、《自然辩证法研究》等核心期刊上发表百余篇学术论文，还主编《技术与哲学研究》、《科技与社会研究》、《技术哲学博士文库》等。现兼任国务院学位委员会哲学学科评议组成员，中国自然辩证法研究会副理事长，技术哲学学会理事长，科学技术与社会学会理事长，辽宁省自然辩证法研究会常务理事长，辽宁省委、省政府咨询委员会委员，沈阳市政府科技顾问，国际技术哲学学会会员等。

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真空冻干产品：小虾、白金虾、南极虾、海虾仁、金钩虾、丰年虾、珍珠虾、小鱼、红赤虫、丰年虫、珍珠虫、轮虫、线虫、豆虫、虫卵、面包虫、棉花虫、水蚤、水苍蝇、蟋蟀、蚕蛹、蚱猛等；颗粒产品：精致脱壳卵、精致龟饲料、小型鱼粮、锦鲤金鱼专用鱼粮、血鹦鹉专用鱼粮、龙鱼专用饲料、七彩专用饲料、高级观赏鱼饲料、增艳高级观赏鱼饲料、海水观赏鱼饲料等；其它产品：鱼药等。

For the showbread, for the daily cereal offering, for the daily holocaust, for the sabbaths, new moons, and festivals, for the holy offerings, for sin offerings to make atonement for Israel, and for every service of the house of our God.

备办供饼、恒常祭品、全燔祭品，安息日、月朔和庆节应献的祭品和各种祭物，为以色列赎罪的赎罪祭品，以及我们天主殿内的一切工作费用。

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Feiyes.net ★ rodick制作原因很难对我来说，失去在我的生命中我发现只有时间才能告诉我们我将找出我们能够婴儿我们可以做一个一夜情，是啊我们很难对我来说，失去在我的生命我发现你的皮肤外面附近的火灾我们能够婴儿我们可以改变和感觉好吗我有点用以外的雨游荡你可以离开我明天如果适合你一样但我不知道不够，我需要有人谁离开天嘿是啊原因很难对我来说，失去在我的生命中我发现只有时间才能告诉我们我将找出我们能够婴儿我们可以做一个一夜情，是啊我们很难对我来说，失去在我的生命我发现你的皮肤外面附近的火灾我们能够婴儿我们可以改变和感觉好吗免费环路-丹尼尔Powter 原因很难对我来说，失去在我的生命中我发现只有时间才能告诉我们我会弄清楚，我们才能婴儿我们可以做一个一夜情，是啊我们很难对我来说，失去在我的生命我发现你的皮肤外面附近的火灾我们能够婴儿我们可以改变和感觉好吗原因很难对我来说，失去在我的生命中我发现只有时间才能告诉我们我将找出我们能够婴儿我们可以做一个一夜情，是啊我们很难对我来说，失去在我的生命我发现你的皮肤外面附近的火灾我们能够婴儿超过。。。

Article 8 For national focal point shelter belt, and forest for special uses, the competent forestry authority of State Council shall produce opinions and report to the State Council for approval, then promulgate it; for local key shelter forest and forest for special use, the competent forestry authority of the people's government of a province, autonomous region or municipality directly under the central authority shall produce opinions and report to the people's government at the same level for approval, then promulgate it; for other shelter forest, timber forest or special-use forest as well as economic forest or fuel forest, the competent forestry authority of people's government at county level shall demarcate according to the state's relevant regulations on forest categorization and the arrangement and organization of the People's government at the same level, and report to the people's government at the same level for approval and promulgation.

第八条 国家重点防护林和特种用途林，由国务院林业主管部门提出意见，报国务院批准公布；地方重点防护林和特种用途林，由省、自治区、直辖市人民政府林业主管部门提出意见，报本级人民政府批准公布；其他防护林、用材林、特种用途林以及经济林、薪炭林，由县级人民政府林业主管部门根据国家关于林种划分的规定和本级人民政府的部署组织划定，报本级人民政府批准公布。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。