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The results are as follows: 37℃ is the most favorable temperature for induction expression of SUMO-hEGF in BL21, 5.0g/L the most best concentration of arabinose, and 4h the best time for induction expression. Meanwhile, the quantity of expression is around 20.2%. The conclusion that hEGF is the purified protein is verified by Western blotting. It is a good foundation for the development of hEGF genetic medicine.

结果表明： SUMO-hEGF在BL21中的最佳诱导表达温度为37℃、诱导剂阿拉伯糖的最佳浓度为5.0g/L、最佳诱导表达时间为4h，表达量约为20.2%；Western blot 分析证实，纯化后的蛋白是hEGF，为进一步开发hEGF基因工程药物奠定了基础。

What is best for a co-worker or neighbor might not be the best choice for you.

什么是最好的一个共同工作者或邻居可能不是你的最佳选择。

In chapter three, we prove that there exist solutions to the Ky Fan variation inequality, as the set-valued mappings are defined on spheres in infinite dimensional Banach spaces or odd dimensional Euclidean spaces, following from these theorems, we obtain some fixed point theorems for set-valued mappings defined on a sphere. When G is an approximate compact convex subset of E, or G is a almost quasi-convex set-valued mapping, we prove that there exist solutions to and type generalized Ky Fan variation inequality, following these theorems, we prove several best approximation theorems and coincidence theorems involving two set-valued mappings and two different spaces. In chapter four, we first present a new Simplicial algorithm for computing the Leray - Schauder fixed points, the algorithm can solve the set-valued nonlinear complementarily problem. We give a condition to guarantee the computation proceeding in a bounded region. We present integer-labeling algorithms for computing fixed points of some set-valued mappings, the best approximation points and solutions to a kind of set-valued variation inequalities.

第四章给出了计算定义在非凸集上的非自映射的Leray-Schauder不动点的算法，而现有的不动点算法都是计算凸集的上半连续集值自映射的不动点；给出了保证计算有界的一个充分条件，我们的条件大大弱于Mdrrill条件，我们的算法也可用来计算Eaves不动点；给出了集值非线性互补问题存在解的一个充分条件，此时可利用Leray-Schauder不动点算法来求解；向量标号算法以往是计算集值映射不动点的唯一有效算法，我们给出用整数标号算法计算一类集值映射的Kakutani 不动点的算法；定义在紧凸集上的连续映射不一定有不动点，但一定有最近点，最近点是不动点概念的推广，我们给出了计算最近点的算法；集值映射变分不等式尚无有效的求解算法，我们给出求解一类集值映射变分不等式的算法。

The Daylight Savings flag uses 1 for DST, 0 for Standard Time, and -1 for best guess.

的日光节约使用国旗1对DST的，0为标准时间，并为best-1猜测。

Up to this time, the major challenge to sign language recognition is how to develop approaches that scale well with increasing vocabulary size In this paper, an approach to large vocabulary, continuous Chinese sign language recognition is presented, which uses subwords instead of whole signs as the basic units Since the number of subwords is limited, HMM based training and recognition of the CSL signal become more tractable and have the potential to recognize enlarged vocabularies Furthermore, the proposed method facilitates the CSL recognition when finger alphabet is blended with gestures About 2400 subwords are defined for CSL One HMM is built for each subword, and then the signs are encoded based on these subwords A decoder that uses tree structured network is presented Clustering of the Gaussians on the state, language model and N best is used to improve the performance of the system Experiments on a 5119 sign vocabulary are carried out, and the correct rate is over 90% for continuous sign recognition

迄今为止，手语识别面临的最大问题是如何解决词汇集易扩充的连续识别提出一种大词汇量连续中国手语识别方法，将词根作为识别基元，由于基元的数目是有限的，因此基于HMM的手语信号的训练和识别变得比较容易处理，可以实现更大词汇量的识别除此之外，所提方法还有利于实现手势语和手指语的混合识别从中国手语中共整理出2 4 0 0多个词根，为每个词根建一个并行的HMM模型，对各数据流的HMM模型进行聚类，确定出手语识别的基元根据这些基元对手势词编码，并建立了树状搜索网格，使用状态结点上高斯密度函数聚类、语言模型和N Best方法提高系统的速度和精度对 5 119个手语词做了实验，连续语句的识别率可在 90 %以上

Here, in the home-brewed world of a midnight hacker, was the first bit of testable proof for hoary ecological wisdom: that what is best for an individual ain't necessarily best for the species.

在午夜黑客自行酝酿的世界中，对古老的生物智慧有这样一个最重要的检验证明：对个体而言是最好的，对物种而言不一定是最好的。

Learn what joint pain relief product is best for you and which named brands are going to be the best for you.

了解关节疼痛救济产品是最适合你，并命名的品牌将成为最适合您。

A paean to the famed auteur who 50 years ago scored an Oscar trifecta for " The Treasure of the Sierra Madre": Best Director, Best Screenplay, and Best Supporting Actor.

他是奥斯卡的奇迹，他的家庭被奥斯卡的光环所笼罩，从来没有哪个家庭祖孙三代都获得过奥斯卡。

No mercy for the world in 1989 Venice Film Festival Special Jury Prize in 1989 Caesar Award for Best Debut Award for best male newcomer idle a living in Paris, but can often favored by young girls,...

没有怜悯的世界 1989年威尼斯电影节评审团特别奖1989年恺撒奖最佳处女作最佳男新人奖一个住在巴黎的游手好闲却又能经常被女生青睐的年轻人，。。。

Marry a person who likes talking; because when you get old, you'll find that chatting[13] to be a great advantage; Find time for yourself. Life will change what you are but not who you are; Remember that silence is golden; Read more books and watch less television; Live a noble and honest life. Reviving[14] past times in your old age will help you to enjoy your life again; Trust God, but don't forget to lock the door; The harmonizing[15] atmosphere of a family is valuable; Try your best to let family harmony flow smoothly; When you quarrel with a close friend, talk about the main dish, don't quibble[16] over the appetizers[17]; You cannot hold onto yesterday; Figure out the meaning of someone's words; Share your knowledge to continue a timeless tradition; Treat our earth in a friendly way, don't fool around[18] with mother nature[19]; Do the thing you shod do; Don't trust a lover who kisses you without closing their eyes; Go to a place you've never been to every year. If you earn much money, the best way to spend it is on charitable deeds while you are alive; Remember, not all the best harvest is luck; Understand res completely and change them reasonably; Remember, the best love is to love others unconditionally rather than make demands on them; Comment[20] on the success you have attained[21] by looking in the past at the target you wanted to achieve most; In love and cooking, you must give 100% effort… but expect little appreciation.

找一个你爱聊的人结婚；因为年纪大了后，你会发觉喜欢聊天是一个人最大的优点；找点时间，单独呆会儿；欣然接受改变，但不要摒弃你的个人理念；记住：缄默沉静是金；多观点书，少观点电视；过一种高尚而诚实的生活当你年老时来回想起过去，你就能再一次享受人生相信上帝，但是别忘了锁门；家庭的融洽氛围是难能可贵的；尽你的能力让家平顺和谐；当你和你的亲近的人吵嘴时候，试着就事论事，不要扯出那些陈芝麻、烂谷子的事；不要摆脱不了昨天；多注重言下之意；和别人分享你的知识，那才是永恒之道；善待我们的地球，不要愚弄自然母亲；做自己该做的事；不要相信接吻时从不闭眼的伴侣；每年至少去一个你从没去过的地方如果你赚了很多钱，在活着的时候多行善事，这是你能得到的最好往返报；记住有时候，不是最好的收获也是一种好运；深刻理解所有的规则，合理地更新他们；记住，最好的爱存在于对别人的爱胜于对别人的索求之上；来回头观观你发誓取得的目标，然后评价你到底有多成功；无论是烹饪不是爱情，都用百分之百的负责态度对待，但是不要乞求太多的往返报很抱歉，因为您在网易相册发布了违规信息，账号被屏蔽被屏蔽期间他人无法访问您的相册

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。