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III For constructing the expression vector of a fusion protein and obtain a target protein with full identity on aa sequence of a natural 13- 1,3-1 ,4-glucanase, with the recombined plasmid DNA harbouring the target gene as template, the primers designed with restriction sites for both terminals and enterokinase recognition site, followed by PCR amplification, was induced to the target gene.

为构建融合蛋白表达载体和获得与天然蛋白质序列完全一致的目的蛋白，以含有目的基因的重组质粒DNA为模板，设计引物时加入两端酶切位点及肠激酶裂解位点，通过PCR扩增引入目的基因中，测序结果表明接头和读框正确。

RESULTS: After 34 weeks, patients occured varying degrees of swelling of the eyelids. Incomplete eyelid closure dribbled away. Followed up for 418 months,doubleeyelids of patients were basic symmetry; the arcs were smooth; ptosis was corrected; depression of the upper orbital region was improved obviously; and no complications appeared, such as entropion and ectropion of the eyelid and eyelid deformity.Only 1 eye was rectified insufficiently.

结果：术后3～4wk内患者均有不同程度眼睑肿胀，眼睑闭合不全逐渐消失，随访4～18mo，患者双眼睑重睑基本对称，弧度流畅，上睑下垂矫正，上眶区凹陷明显改善，未出现眼睑内外翻及眼睑畸形等并发症，有1眼欠矫。

They entered the property and followed the internal road until the entryway of the house.

她们进入了庄园，沿着主干道来到房子的入口通道。

We studied an additional 7 rabbits after left entricular endocardial cryoablation, resulting in a thin layer of suriing epicardium. Cai sinkholes occurred 31±12 ms after the shock, followed in 19±7 ms by first postshock actiation in 63 episodes of unsuccessful defibrillation.

我们进一步研究了左室内膜冷冻消融后的7只兔，Cai穴发生在电击后31±12ms，在63次除颤未成功情况下，首次电击后激活出现在电击后19±7ms的Cai穴。

There have of course been innumerable obesity treatments and diets, and the sad thing is people by and large go back to their original weight,' says Martijn Katan, professor of nutrition at Vrije Universiteit Amsterdam, who has followed Epode.

一直跟踪Epode项目的阿姆斯特丹自由大学营养学教授卡坦说，市面上当然有许许多多的肥胖疗法和减肥食谱，但令人遗憾的是，人们大多还是反弹回了从前的体重。

"There have of course been innumerable obesity treatments and diets, and the sad thing is people by and large go back to their original weight," says Martijn Katan, professor of nutrition at Vrije Universiteit Amsterdam, who has followed Epode.

明星美容K饮食才能减肥K一直跟踪Epode项目的阿姆斯特丹自由大学营养学教授卡坦说，市面上当然有许许多多的肥胖疗法和减肥食谱，但令人遗憾的是，人们大多还是反弹回了从前的体重。

Her eponymous debut album was released to major acclaim in 1971 and was followed two years later by Heart Food.

她的名字命名的首张专辑被释放到1971年的主要赞誉，并在两年后，由心食品。

This is denoted by an operator followed by an equal sign, and is consistent with all the operators in the language.

这是由等号前的一个运算符标记的，而且对于语言中的所有运算符都是固定的。

In the property settings box, type an equal sign followed by the expression.

在 方块中键入一个等号，并在后面键入运算式。

Each member initializer must name an accessible field or property of the object being initialized, followed by an equals sign and an expression or an object or collection initializer.

每个成员初始化器必须指出正被初始化的对象的域或属性的名字，后面是等号"="和表达式或者对象或集合的初始化器。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。