查询词典 fluorogenic

Methods and Results— The NIRF imaging agent consists of the CatK peptide substrate GHPGGPQGKC-NH2 linked to an actiatable fluorogenic polymer.

方法及结果：短距红外线荧光显影剂含有组织蛋白酶K缩氨酸感光底物GHPGGPQGKC-NH2，其连结在一个可激活的荧光底物激活体上。

We synthesized a new long-wavelength latent fluorogenic probe BQC (1) to monitor DT Diaphorase activity.

设计合成对於还原酵素具有专一作用之新隐藏型长波长萤光探针BQC。

Methods and Results— The NIRF imaging agent consists of the CatK peptide substrate GHPGGPQGKC-NH2 linked to an activatable fluorogenic polymer.

方法及结果：短距红外线荧光显影剂含有组织蛋白酶K缩氨酸感光底物GHPGGPQGKC-NH2，其连结在一个可激活的荧光底物激活体上。

Fluorogenic and HPLC assays using isolated microsomes showed robust catalytic activity of the prototype CYP 3A4 enzymes, and the values of Vmax and Km were comparable to the published data.

另外，与CYP3A4野生型相比，突变株R162Q，L293P，M445T和P467S代谢底物的能力没有显著改变，而T185S，F189S和S222P的活力均有不同程度的下降，其中F189S的活力最低，达到CYP3A4野生株的1/10。

Methods A novel fluorogenic probe, TaqMan-Molecular Beacon, which combined the hairpin structure of molecular beacons and the cleavage mechanism of TaqMan probes,was developed. Combined with a real-time PCR instrument, TagMan-MB was used for quantification of templates of ancient DNA.

利用分子信标的茎环结构和TaqMan的降解作用设计一种新型的TaqMan-Molecular Beacon荧光检测探针，与实时PCR仪联用，对古DNA模板进行定量检测。

Second, investigations were made in the effects of polymerization of HRP on its catalytic activity in the excess of H〓O〓 by the use of fluorogenic hydrogen donors of HRP. The results showed that some HD, e. g. homovanillic acid could indicate the activity differences between HRP and polymerized HRP.

其次，为建立辣根过氧化物酶标记的调制型荧光免疫分析研究了用荧光供氢体代替显色供氢体指示聚合前后辣根过氧化物酶在高浓度H〓O〓中活性的差异。

Methods The system employs the 5' 3' nuclease activity of Taq DNA polymerase to digest a salmonella specific internal fluorogenic probe which is labeled with two fluorescent dyes and hybridizes to the target DNA during PCR.

利用TaqDNA聚合酶的 5' 3'核酸外切酶活性降解特异性杂交于模板上的荧光标记探针，导致荧光强度的改变，通过检测荧光强度分析PCR扩增产物。

Methods Gastric cancer tissues, adjacent tissues and normal gastric mucosal tissues in 75 patients were studied, The expression of ubiquitin and 26S protesome activity were analyzed by Wes tern blot and fluorogenic technique, res pectively.

对75例胃癌患者分别在胃癌、癌旁及远端正常黏膜组织取样进行研究。采用免疫印迹方法分析泛素表达，免疫荧光方法分析265蛋白酶体活性。

In order to meet the analysis requirement, this article has introduced the main technological method and concrete application of the independently developed isotope X fluorogenic analysis instrument. The practical application effect indicated this analyzer when carries on the on-line survey to industry coating and automobile coating thickness its measuring accuracy may reach 2%~4%.

众所周知，产品和金属元件表面镀层或防腐层厚度是与产品质量与性能相关的重要指标，为满足分析要求，采用自行研制的同位素X射线荧光分析仪，对工艺铁基镀锌、铁基镀镉和汽车涂层3类样品以美国NBS系列标准为参照，采用发射法和吸收法进行了应用实验，并对在线镀锌板生产线进行了实验。

And the IC50 values of all tested compounds did not change at various times. The panel of CYP3A4 polymorphic enzymes will be tested against various drugs, kinds of fluorogenic substrates and CYP3A4-specific substrate to identify potentially adverse drug-CYP in order to generate predictive information applicable in personalized medicine.

多种荧光底物、探针底物和大量的上市药物对人细胞色素P450 3A4野生株及其突变株的体外代谢研究正在进一步进行中，旨在建立一种高通量、简便、快捷的针对于CYP3A4遗传多态性的药物筛选平台，从而为临床个体化用药提供科学依据。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。