查询词典 fluorogenic

The thesis focuses on gene diagnosis of diseases, and six new homogenous fluorogenic probes are developed. Using hepatitis B virus in human sera as targets, the detection effects of these probes are discussed.

本文致力于疾病的基因诊断研究，开发了六种新型的均相荧光基因检测探针，并以病人血清中的乙型肝炎病毒为检测对象，对上述六种探针的检测效果进行了评估。

Methods The expression of CK20 mRNA in the peripheral blood was determined with fluorogenic quantitative RT-PCR in 40 cases of panereatie carcinoma in the night before operation, 5 cases of benign pancreatic diseases and 5 healthy individuals.

方法用荧光定量RT-PCR检测40例作根治术的胰腺癌病人外周血中CK20mRNA，并与病人的临床病理学指标、随访结果进行相关性研究。

Expression of MDR1 gene was measured with the fluorogenic quantitative RT-PCR method in 49 cases of breast cancer and 16 cases of control group (including 8 cases of benign breast lesions and 8 cases adjacent breast tissues). In addition, immanohistochemical analyses was used to detect the expression of P-gp and C-erbB-2 protein in above mentioned tissues.

采用荧光定量RT-PCR法检测49例乳腺癌，16例对照组(包括8例乳腺良性疾病、8例癌旁组织)中MDR1基因的表达，同时采用免疫组化检测表述标本中P-gp及C-erbB-2蛋白的表达。

MethodsThe frequency of CD4+CD25+T cells in the peripheral blood mononuclear cells from 30 cases of severe hepatitis B, 20 cases of chronic hepatitis B, 20 cases of chronic asymptomatic HBV carriers and 10 healthy controls were determined by flow cytometry and their serum HBV DNA levels were detected by Quantitative Fluorogenic PCR.

方法采用流式细胞仪检测30例重型乙型肝炎患者、20例慢性乙型肝炎患者、20例无症状乙肝病毒携带者和10例健康的外周血CD4+CD25+调节性T细胞（CD4+CD25+Treg）的水平，并应用荧光定量PCR方法测定上述研究对象血中HBV DNA滴度。

Methods Using LineGene fluorogenic quantitative PCR detection system to detect HBV DNA of 77 patients and second derivative maximum method and fit point method to analyze results.

用LineGene FQD33A型荧光定量PCR检测系统检测本院门诊和病房乙肝病人血清HBV DNA 77份，分别用最大二阶导数法和样点拟合法进行结果分析。

Liver disease ; alcoholic ; Low molecular weight polypeptide7; Carbonyl stress ; Polymerase chain reaction ; Fluorogenic quantitative

酒精性肝病；低分子量多肽7；羰基应激；聚合酶链反应；荧光定量

9Ug/ml. And in the same specimens, the mdrl gene were detected using the methods of fluorogenic quantitative PCR.

并对同一标本提取其乳腺癌细胞RNA，采用荧光定量PCR方法进行mdr1 mRNA检测。

Fluorogenic quantitative polymerase chain reaction is an emerging technique with high specificity, susceptibility, automatism and has been widely used for the detection and quantification of microorganism, some genetic diseases and cancer.

定量PCR技术以其高特异性、高灵敏性以及高自动化等特点在临床病原微生物诊断、部分遗传性疾病诊断和癌症辅助诊断等方面得以广泛应用。

Based on these results and problems, develop a fluorogenic imaging agents, which emitting in the near-infrared, will be predicted to improve the problems.

另外，用自我焠熄的作用，设计合成双近红外线葡萄醛酸(Di-IR-820 glucuronide)前探针增加敏性，低背景干扰。

Enzymatic activity of three main hydrolases in proteasome was measured by detection of the fluorophore of various cleavaged synthetic fluorogenic peptides.

荧光分光光度计测量各组细胞内蛋白酶体水解酶活性的变化。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。