查询词典 fluorescent

The fluorescent response of BQC is insensitive to various biological thiol reductants.

对生理环境中所存在的各种不同硫化物做还原的测试，隐藏型萤光探针不会发生氧化还原反应，依然保持专一的性质。

Phycobiliproteins are brilliantly coloured,intensely fluorescent chromoproteins,which consist of apoproteins and covalently bound linear tetra pyrroles as prosthetic groups.

藻胆蛋白是藻类光合作用捕光复和物的功能组分，所以研究藻胆蛋白生物合成对认识光合作用的形成和机理非常重要。

In order to develop new DNA probes,we have synthesized two kinds of probes bipyrene ratiometric fluorescence probes and long wavelength Ruthenium polydiimine complex,the following work have been carried out:Fluorescent DNA probes with 1,6-hexanediyl as the linker between two pyrenes, phenylpyrenes or phenylethynyl pyrene fluorophores were synthesized(Py-1,Py-2 and Py-3) and their interactions with DNA were studied by UV-Vis absorption spectra,fluorescence spectra and viscosity measurements.

在前人的基础上，为了更好的识别DNA，本论文设计合成了两类DNA探针——双芘类比率型DNA荧光探针和长波长钌的配合物，具体如下：以己二胺为柔性链，以芘及其衍生物为发色团合成了探针Pyl-3，并通过荧光光谱，荧光紫外吸收光谱和黏度测试研究了探针与DNA的结合方式。

Enhanced green fluorescent protein-tagged ICL was distributed in a punctate pattern at putative inhibitory synapses,identified by vesicular GABA transporter immunoreactive puncta.

免疫组化实验证明了EGFP-ICL是以PUNCTA的形式分布于抑制性神经元的树突上。

This project will mass produce high purity paralytic shellfish toxins using a combination of photobioreactor, HPLC-MS and NMR approaches, and develop novel PSTs detection method based on fluorescent characteristics of crown ethers, and finally apply this product and method for PSTs monitoring in

本项目将分离纯化中国沿海典型麻痹性贝毒C1/C2，GTX1/4，GTX2/3和GTX5，运用液相色谱-质谱联用技术和核磁共振等方法定量毒素纯度和含量，制备高纯度毒素标样，建立麻痹性贝毒的大规模生物生产技术；合成筛选适宜的毒素化学传感器—冠状醚，优化毒素和冠状醚的反应体系，结合多重时间分辨荧光技术，建立麻痹性贝毒多重时间分辨荧光检测技术，并用于水产品中麻痹性贝毒的检测。

This project will mass produce high purity paralytic shellfish toxins using a combination of photobioreactor, HPLC-MS and NMR approaches, and develop novel PSTs detection method based on fluorescent characteristics of crown ethers, and finally apply this product and method for PSTs monitoring in seafoods.

本项目将分离纯化中国沿海典型麻痹性贝毒 C1/C2 ， GTX1/4 ， GTX2/3 和 GTX5 ，运用液相色谱-质谱联用技术和核磁共振等方法定量毒素纯度和含量，制备高纯度毒素标样，建立麻痹性贝毒的大规模生物生产技术；合成筛选适宜的毒素化学传感器—冠状醚，优化毒素和冠状醚的反应体系，结合多重时间分辨荧光技术，建立麻痹性贝毒多重时间分辨荧光检测技术，并用于水产品中麻痹性贝毒的检测。

The PCR products were labeled with the fluorescent DNA dye SYBR green. The amount of burden or load was measured by GeneAmp 5700 Sequence Detection System. Using this method, the HIV 1 proviral burdens in PBMC of patient and in cell suspension treated with the compounds AZT, GL and WT were measured.

以病毒感染细胞和培养上清为材料，测定了三种化合物对细胞内的前病毒载量和培养上清中的病毒载量的抑制活性，并与合胞体形成抑制方法测定化合物抗病毒活性的结果进行了比较。

The BDDcFⅧ gene was ligated behind PUB and 20H1 promotors to create lentiviral vectors pTK161 and pTK162. Meantime lentiviral vectors pTK161' and pTK162' were produced by cloning a green fluorescent protein into pTK151 and pTK152, which was driven by PUB and 2OH1 promotors respectively. Vector supernatant were prepared by using transfer calcium phosphate mediated-cotransfection of 293T cells. The virus vector,△NRF packaging-plasmid, and VSV-G envelope-plasmid was assayed by titers and cFⅧ activity in cell culture supernatant after infection into 293T cells.

用构建携带cFⅧ基因的慢病毒载体pTK161和pTK162（含20HI启动子），同时构建含绿色荧光蛋白的慢病毒载体pTK161'和pTK162'（含2OH1启动子）的方法，分别与包装质粒△NRF、包膜蛋白质粒VSV-G共转染293T包装细胞，将包装好的病毒颗粒再感染293T细胞，检测病毒滴度和培养细胞上清中cFⅧ活性。

METHODS: The expression of nucleostemin in 67 acute leukemia patients was detected by fluorescent quantitative polymerase chain reaction.

采用荧光定量PCR的方法检测67例急性白血病患者骨髓细胞nucleostemin基因表达，分析其表达与临床的关系。

I kept evolving more elaborate floral patterns, until another visitor shifted the direction toward wild fluorescent plaids.

我不断进化出更多复杂的植物图案，直到另一个参观者把方向改变成强烈的荧光格子花纹。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。