查询词典 fluorescent

As the development of monoclonal antibody and quantitated fluorescent cytochemistry technique, FCM has been used more extensively in biology, clinical medicine, pharmacology, material and so on.

本文就流式细胞术的发展历史、流式细胞仪的原理及在各领域的应用进行综述，阐明现代流式细胞术由于结合单克隆抗体技术、定量荧光细胞化学技术，使其在生物学、临床医学、药物学、材料学等众多研究领域中的应用有更加突飞猛进的发展。

"Our commitment is to offer the most advanced image analysis systems to assist the pathologist and cytogeneticist in classifying and counting cells of interest, utilizing our advanced algorithms for both brightfield and fluorescent scanning," noted Opher Shapira, president and CEO of BioView.

BioView总裁兼首席执行官Opher Shapira指出：我们的承诺是提供最先进的图像分析系统，用以帮助病理学家和细胞发生学家，通过使用我们的高级运算法则，在光学视野和荧光扫描图像之中，对感兴趣的细胞进行分类和计数。

Deepfreeze, fluorescent light source.

D 水马达、小水浴箱、冰箱藏室和日光灯管。

The result show that the precipitated CSFV fluorescent antibody with high sensitivity and specificity and can be used in the clinical CSFV depuration of tonsil tissue detection.

结果表明，本实验所制备的猪瘟荧光抗体具有很高的灵敏性和特异性，可用于猪瘟净化中扁桃体组织抹/涂片检测。

The methods of DNA depuration and detection are proved to be simple,quick and accurate, which establish a good foundation for the assessment of DNA vaccine safety,and have the favourable perspective in application.

荧光定量PCR（Fluorescent Quantative Polymerase Chain Reaction，FQ-PCR技术是美国PE公司研制成功的一种高灵敏度和高精确度核酸检测方法，该法在传统PCR的基础上引入荧光标记探针，从根本上解决了PCR反应中非特异性扩增的难题[4]。

Methods］ A total RNA from 10 cases withovarian papillary serous cystadenocarcinoma and normal ovarian tissues were extracted by a single step method of gene chip. The cDNA retro?鄄transcribed from equal quantity mRNA derived from 10 cases with ovarian papillary serous cystadenocarcinoma and normal ovarian tissues. The cDNA strand was labeled with fluorescent Cy5 and Cy3(normal ovarian epithelial cells) as probes.

方法］ 采用基因芯片技术，按一步法分别抽提10例卵巢浆液性乳头状囊腺癌患者的癌组织和正常卵巢组织的总RNA并纯化mRNA；逆转录合成以Cy5荧光标记卵巢癌细胞和Cy3荧光标记正常卵巢上皮细胞的cDNA作为探针，在含有8 464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

After developing 48 hours, hepatocytes enters 2.0 mM t-butyl hydroperxide or 2.0 mM diamide to induced cell blebbing. The samples collected at 0, 15, 30, 60 minutes to analyze cell about membrane protein thiol deletion; the leakage of lactate dehydrodenase; the change of glutathione; the lipid peroxidation; the variation of actin and tubulin, and use Confocal fluorescent microscope to observe the change of actin and tubulin in rat hepatocytes.

肝细胞在48小时培养后，分别加入2.0mM t-buty1 hydroperoxide或2.0 mM diamide诱发细胞膜小泡生成，并分别於0、15、30、60分钟取样，分析细胞膜硫醇流失程度；乳酸去氢□渗漏；□胱甘□浓度变化；脂质过氧化；肌动蛋白与管蛋白变化；并利用雷射共轭焦萤光显微镜观察肝细胞中肌动蛋白及管蛋白的变化。

The result indicates the t-BHP or diamide both cause the mass loss about the membrane protein thiol in hepatocytes, the fast oxidization about glutathione in hepatocytes, the leakage of lactate dehydrodenase, but the level of t-BHP is larger than of diamide. In the aspect of lipid peroxidation, only the t-BHP produces apparent change. As to actin and tubulin, they both find fall-off and present time-dependency effects. The result in Confocal fluorescent microscope also show these two cytoskeletal proteins are related to cell blebbing, but they don''t play the same role during the porcess. Tubulin may be more important than actin. If the study predispose dithiothreitol before adding t-BHP or diamide, then the damage by the diamide is repressed, but just partly protection for the damage by t-BHP.

结果显示t-BHP或diamide均可造成细胞膜蛋白硫醇大量流失与胞内GSH快速氧化，亦显著导致乳酸去氢□渗漏，但t-BHP诱发的渗漏程度大於diamide；在脂质过氧化方面，则仅t-BHP处理组产生明显的脂质氧化情形；至於肌动蛋白与管蛋白单体均可见减少且呈现时间依赖效应；在雷射共轭焦萤光显微镜观察中，亦显现这两种架构蛋白与细胞膜起泡有关，但两者在小泡生成的过程中扮演的角色并不完成相同，可能管蛋白较肌动蛋白重要；如果细胞在t-BHP或diamide加入前预先以dithiothreitol处理，则diamide 所造成的伤害显著的受到抑制，但DTT对t-BHP所诱发的伤害则仅有部分保护效果。

Then select the very surfactants\' concentration to treat SBP for one hour, scan on the circular dichromatism spectrum and fluorescent spectrum; ultraviolet-visible spectrum scans SBP\'s variations within 2h after surfactants\' adding to immediately.

实验前加适量相应缓冲液溶解使SBP浓度为250mg/ml.30℃下，设置三种表面活性剂的浓度梯度，使得每种表活剂的加入使得SBP酶活力变化，并在某一特定浓度和特定时间残留酶活力稳定。

It is found that the fluorescence intensity is increased with the elevation of temperature when the quencher diphenylamine is added to PU-DFY, which is an inverse phenomenon against the general fluorescent materials.

研究还发现，加入二苯胺猝灭剂的PU-DFY，荧光强度随温度的升高而增强，与一般荧光物质荧光强度的温度特性相反。关键词：聚氨酯-分散荧光黄；高分子荧光染料；水分散体；荧光

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。