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Fluorescence in situ hybridization and double FISH experiments were carried out to ascertain the chromosomal distribution patterns of the 45S and 5S ribosomal DNAs in the three species of Cucurbitaceae.

首次利用荧光原位杂交和双色荧光原位杂交技术对45S和5S rDNA在南瓜、丝瓜、冬瓜的有丝分裂中期染色体上进行了物理定位分析。

Relation between the composition of photosynthesis pigment and the three dimensional fluorescence spectrum characteristic of each spectral group was analyzed. Selecting pure cultures of cyclotella meneghiniana, chlorella vulgaris and...

组分1、组分2和组分3在模1上得分与其在混合样品中的实际相对浓度具有良好的线性关系，相关系数分别达到0.98222，0.97865和0.99788，证实了利用三维荧光光谱和PARAFAC模型分类测量浮游植物浓度的可行性。

Methods Totally 173 patients diagnosed as MDS according to FAB or WHO criteria with complete clinical and cytogenetical data were included in this reseach. Among them 42 had +8 chromosome aberration, 16 had -7/7q-, and 55 had normal karyotypes and more than 0.10 blast cells in the bone marrow. Short term culture and G-banding techniques and in some specimens fluorescence in situ hybridization method were used to do chromosome analysis.

方法应用骨髓细胞短期培养法和染色体G显带技术，部分病例联合应用荧光原位杂交技术，对42例细胞遗传学检查具有+8异常克隆，16例具有-7/7q-异常克隆，以及55例虽然经常规细胞遗传学检查未检出异常克隆，但是骨髓涂片原始细胞≥0.10，按照FAB或WHO标准既往诊断为MDS的病例进行了临床及血液形态学和细胞遗传学的系列研究。

MAIN OUTCOME MEASURES: Transfection rate and effect of ECV304 proliferation were calculated by fluorescence microscope and flow cytometer.

主要观察指标：①观察转染后对ECV304生长的影响，在流式细胞仪和荧光显微镜下计算转染效率。

In addition, the TIG-310 was fused to GFP and expressed in L cells and COS-7 cells. It seems that the TIG-310 gene may be a cytoplastic protein, for the fluorescence is mainly found in the cytoplasm of the transfected cells.

由于肠粘膜含有多种细胞，其功能又各不相同，我们用原位杂交方法分析肠粘膜中表达TIG-310的细胞，发现该基因主要表达在肠粘膜的上皮细胞。

Methods:TNF-α and TGF-β_1 mRNA expression on placenta decidual tissue from 15 early spontaneous abortion women and 20 normal early pregnant women were examined by real-time fluorescence quantitative RT-PCR.

方法采用实时荧光定量RT-PCR法检测了15例早期自然流产及20例同期正常早孕要求行人工流产者蜕膜组织的TNF-α和TGF-β1mRNA表达水平。

AIM: Using a new laser fluorescence device - the KaVo DIAGNOdent,we studied the occlusive effects of mixed solution of NaHCO 3 and NaF on dentinal tubules.

目的 ：用激光荧光仪定量研究碳酸氢钠和氟化钠混合液对牙本质小管的堵塞作用。

Fluorescein diacetate, which generates fluorescence upon hydrolysis, is used as the assay substrate.

荧光素双乙酸盐在水解时产生荧光，可用于培养基化验。

The interaction of tributyltin, the main components of antifouling paint of ships, and its degradation products of monobutyltin and dibutyltin with human serum albumin was investigated by ultraviolet, fluorescence and circle dichromism spectra. The influences of the concentration, acidity, organic solvent such as ethanol and ionic strength on the interaction of butyltin compounds and HSA molecule were examined to find that the interaction of butyltin compounds and HSA was dual.

通过紫外、荧光和CD光谱，研究了船体防污漆中主要防污成分——三丁基锡化合物及其降解产物——一丁基锡和二丁基锡与人血清白蛋白相互作用的方式与程度，考察了浓度、酸度、有机溶剂和离子强度等因素对相互作用的影响。

The fluorescence images that RhoCr1 coordinated with Cr~(3+)in PC12 cells showed RhoCr1 to be capable of penetrating into living cells and imaging intracellular Cr~(3+)changesFive probes have been designed and synthesized,where different aldehydes were covalently linked to rhodamine B dyes with diethylamine flexible chains.

吸收和荧光光谱的测试结果表明，探针RhoCr1在Tris-HCI中性缓冲溶液中能高选择性的识别Cr~(3+)并产生了荧光增强，通过对探针光谱性能的测试和TEPN滴定实验表明，探针RhoCr1与Cr~(3+)的络合是一个可逆的过程。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。