查询词典 fluorescence

Hematoporphyrin is a kind of auto- fluorescence substance in tumour tissue, it plays important role in diagnosis tumour with in-ducing tissue auto - fluorescence.

血卟啉为存在于肿瘤组织中的自体荧光物质，它在诱导组织自体荧光法诊断肿瘤中起着较为重要的作用。

ObJective To investigate the distribution of hematoporphyrin monomethyl ether in rabbit choroidal melanomas by dye fluorescence angiography and fluorescence microscopy.

目的 应用血管造影和组织冰冻切片荧光显微镜观察的方法，研究国产新型光敏剂血啉甲醚（hernatoporphyrin monomethyl ether，HMME）在兔脉络膜黑色素瘤眼部组织的分布。

Methods Cell culture,immunohistochemistry,nucleus fluorescence labeling,fluorescence retrograde labeling and enzymatic histochemistry technique were used.

应用细胞培养、免疫组织化学、核荧光标记、荧光逆行追踪和酶组织化学等技术。

A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising subjecting said sample to an amplification reaction using a set of nucleotides, at least one of which is fluorescently labelled, contacting amplification product with a probe under conditions in which the probe will hybridise to said target sequence, said probe comprising a reactive molecule which is able to absorb fluorescence from or donate fluorescent energy to said fluorescent labelled nucleotide and monitoring fluorescence of said sample.

一种用于检测在某样品中是否存在目标核酸序列的方法，该方法包括：使用一套核苷酸对所说的样品进行扩增反应，其中至少一个核苷酸是被荧光标记的，在探针与目标序列杂交的条件下使扩增产物与探针混合，所说的探针包括一种活性分子，该活性分子能够从荧光标记核苷酸吸收荧光或向其贡献荧光能量，及检测样品的荧光。

Using 3 hydroxy 2 naphthoic acid as a fluorescence probe, the relationship of the crosslinking degree of the polymer with the stability of supramolecular complex and the fluorescence properties was investigated, respectively.

用改进的Renard方法控制合成了 6种不同交联度的水溶性β环糊精聚合物。

The results indicated that the content of cytosolic CaM in cells treated with exogenous Ca2+ has increased indistinctively, while fluorescence intensity in cells treated with TFP decreased. So we believed that exogenous Ca2+ has little effect on the expression of CaM. High concentration of TFP can enter yeast cells and combine to CaM to make it inactive, which is the reason that TFP restrain the growth of yeast cells. 5mmol/L EGTA could completely arrest the cell proliferation of MFP7 after 28h, when the fluorescence intensity in cells wasobviously increased with flow cytometry and LSCM.

被较高浓度TFP处理过的MFP7胞内荧光强度则相对较弱，推测是因为TFP进入胞内与CaM结合从而使其失活，这也是高浓度TFP抑制酵母细胞增殖的原因。5mmol／L EGTA处理28h左右后，酵母细胞的生长被抑制在G1晚期，此时通过细胞流式法和在激光扫描共聚焦显微镜下观察到胞内荧光强度显著高于对照，说明CaM的表达水平在G1后期开始上升；回加10mmol／L Ca~(2+)处理24h后，细胞开始恢复生长，细胞流式法测定胞内荧光强度有所下降，表明多数细胞完成G1／S转换，进入生长对数期。

Methods The model of intravasation migration of cancer cell in vitro was used. The fibrosarcoma cell HT1080 was labelled with fluorescence, and the fluorescence guantities in 24-pore plate through collagen membrane and HUVEC single cells layer were detected in different time to eveluate the intravasation migration of cancer cell.

利用体外癌细胞向血管内游走模型，将纤维肉瘤细胞HT1080经过荧光标记，通过测定不同时间穿过胶原层及HUVEC单细胞层落到24孔板内的荧光量来定量评价瘤细胞血管内的游走情况。

An fluorescence spectral method is proposed to analyze liverish, nephrotic and diabetic sera. The aim is to find difference between serum of healthy human and liver diseases patients in polarization fluorescence spectra.

本论文应用激光光谱技术，对肝病、肾病及糖尿病患者的血清进行光谱分析，试图探讨正常人与病变患者的血清中的代谢产物在光谱上的差异，从分子水平研究细胞病变的可能机制。

The apoptotic effects of LPS are detected by various skills such as fluorescence staining, TUNEL reaction, DNA agarose gel electrophoresis, electron microscopy and fluorescence activated cell sorting. Our results demonstrated that fish lymphocytes would be apoptotic under different LPS concentrations(above 10μg/ml) and different time(above 6h) inducing, which is characterized by condensation and margination of chromatin, the formation of apoptotic bodies.

结果显示，在10μg/ml以上浓度的内毒素作用18后或以100μg/ml浓度的LPS作用6h以上，淋巴细胞即可出现明显的凋亡形态学变化，表现为染色质凝集和周边化，核断裂，形成凋亡小体，DNA发生特异降解产生典型的"梯状"电泳图谱，呈现TUNEL阳性标记等，并通过流式细胞术证实这种凋亡具有时问和剂量的依赖性。

Some of the cells were as followed:nuclus chromatins were side,nuclear type had several notches,nuclear membranes were clear. 4. The cells that are stained by 1% iodinate tritroche can be observed by fluorescence microscope: Control group:the conformation of nucleous is satiation and present a yellow red color of fluorescence. 1000 U/mlγ- IFN+1μmol/1 ATRA group: the nucleous of most cells are presenting karyopyknosis,becoming punctuate or massiveness and concentrating at one side of the cell to form crescent shape or granular shape.

进一步进行组间比较：实验组与对照组细胞相对抑制率差别均具有显著性(p=0.000＜0.01)。1000U／mlγ-IFN+0.1μmol／l ATRA组与1000 U／mlγ-IFN+0.5μmol／l ATRA组细胞相对抑制率差别无显著性(p=0.391＞0.05)。1000U／mlγ-IFN+1μmol／l ATRA组与1000U／mlγ-IFN+0.1μmol／l ATRA组、1000 U／mlγ-IFN+0.5μmol／l ATRA组细胞相对抑制率差异具有显著性(p=0.000＜0.01)；而与1000 U／mlγ-IFN+5μmol／l ATRA组、1000 U／mlγ-IFN+10μmol／l ATRA组细胞相对抑制率差异无显著性(p值分别为0.294，0.536＞0.05)。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。