查询词典 fluorescence

Keywords Capillary electrophoresis;on-line sweeping concentration;hollow fiber-based liquid-phase microextraction;fluorescence spectroscopy;interaction;serum albumin;Strychnos alkaloids;fangchinoline;tetrandri

毛细管电泳；在线推扫富集；中空纤维液相微萃取；荧光光谱法；相互作用；血清白蛋白；马钱子碱；防己诺林碱；粉防己碱；槲皮素；卡马西平；甲基多巴；左旋多巴

The remote sensing differential information processing technique, the X fluorescence survey of stream sediments and the subaerial base rock gama survey technique were jointly applied in the Puziwan gold deposit, Shanxi to study the gold metallogenic fluid field .

综合运用遥感微差信息处理技术、水系沉积物X荧光测量和地表基岩伽玛能谱测量方法研究了山西堡子湾金矿的成矿流体场，发现主要的矿化蚀变带呈NW向展布，与含矿的NEE向角砾岩带斜交，表明成矿后受到一定程度的改造。

Methods］ MTT assay was employed to test the lethal concentration 50 (IC50) of sensitive germline (SK-OV-3) and ovarian cancer cell subline induced by ADR (SK-OV-3/adr) treated by 4 drugs, resistance index and reverse multiple were calculated. The changes of drug content were observed by fluorescence microscope, the intracellular accumulation of ADR was evaluated by fluorospectrophotometry, the changes of mdr 1/P-gp resistance were observed respectively by RT-PCR and Western blot.

方法］MTT法检测药物对敏感细胞株（SK-OV-3）和诱导的耐药细胞株（SK-OV-3/adr）的半数致死浓度（IC50），计算耐药指数和加逆转剂后的逆转倍数，荧光显微镜观察不同时间细胞内的药物含量变化，荧光分光光度法测定细胞内ADR的含量，RT-PCR和Western blot法检测细胞耐药前后mdr1/P-gp的变化。

Methods Fluorescence in situ hybridization with a whole chromosome 7 painting probe and a 7q subterminal probe (7q36→qter), prepared by chromosome microdissection technique, was used to analyze a case with a history of spontaneous abortion and 7q terminal deletion detected by conventional G-banding technique.

显微切割制备7号和7q亚端粒(7q36→qter)探针，通过荧光原位杂交技术分析一例具有反复流产史且常规G带发现有7q末端缺失的病例。

A series of cholesterol-bearing O-carboxylmethyl chitosan conjugates with different degrees of substitution of the cholesterol moieties and cholesterol-bearing chitosan were synthesized by the succinyl linkages and characterized by Fourier transform infrared, Proton Nuclear Magnetic Resonance (~1H NMR) and elemental analysis. These hydrophobized chitosan derivatives were amphiphilic in nature and their self-aggregation behavior in aqueous media was evaluated by the fluorescence probe technique.

课题的主要研究内容如下：采用化学的方法合成了不同分子结构、不同取代度的系列胆甾醇基壳聚糖衍生物—胆甾醇基壳聚糖和胆甾醇基-羧甲基壳聚糖，并应用先进的分析手段电位滴定、红外光谱法(IR、核磁共振波谱法等对原料、中间产物以及产物进行了结构表征，衍生物分子中的胆甾醇基取代度(3.2％～12.5％)采用元素分析的方法进行检测。

Self-assembled nanomicelles of N -cholesterol succinyl O -carboxymethyl chitosan were prepared by probe sonication method. The amphiphilic property and the critical micelle concentration of CCMC were determined by fluorescence probe technique; the morphology and the size of CCMC self-assembled nanomicelles were analyzed by transmission electron microscopy and the dynamic laser light scattering.

以 N -胆甾醇琥珀酰基- O -羧甲基壳聚糖(CCMC，胆甾醇基取代度6.9%)为原料，在水溶液中通过探头超声处理制备其自组装凝胶纳米胶束，采用稳态荧光探针法考察临界胶束浓度，并通过透射电镜和动态激光散射仪检测胶束的形态大小。

A fluorescence spectrophotometric method was developed for the determination of sulfadiazine based on a charge transfer reaction.

用荧光法研究了乙酰丙酮-甲醛与磺胺嘧啶的荷移反应的条件，并探讨了发光机理。

In the second part of the thesis, we developed a very effective assay for the sulfotransferase that catalyzes the sulfation of serine and threonine. It utilized PAPS regenerated from 3-phosphoadenosine 5-phosphate by a recombinant PST using 4-methylumbelliferyl sulfate as the sulfuryl group donor. The change in the fluorescence intensity of 4-methylumbelliferone corresponded directly to the amount of active protein sulfotransferase.

第二部份则是探讨蛋白质亚硫酸转移酵素的活性检测方法，检测系统的主旨是用重组的PST催化受质4-methylumbelliferyl sulfate做为亚酸基提供者并转移至3'-phosphoadenosine 5'-phosphate再生PAPS ，此核苷酸的亚酸基再由蛋白质亚硫酸转移酵素催化，反应后所产生的4-methylumbelliferone之萤光变化可用来定量蛋白质亚硫酸转移酵素的活性。

Basing on the fast Fourier transform.The fluorescence lifetime is obtained from the tangent function of the phase angle of the first nonzeroth items of the FFT result.This method has advantages such as quick calculation,high accuracy and immunity to the base signal.

基于快速傅里叶变换的拟合方法，从第一个非零项的相位角的正切值得出被测的荧光寿命，具有速度快、误差小、不受本底干扰等优点。

In succession, the middle layer of anther wall had the strongest Ca^2＋ fluorescence intensity, and the tapetum took second place during the meiosis of microspore mother cell.

小孢子内Ca^2＋分布不均匀，细胞质中的Ca^2＋荧光较弱，细胞壁处Ca^2＋荧光较强，二细胞花粉时期，细胞呈现较强的Ca^2＋荧光。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。