查询词典 fluorescence

In the biochemical analysis aspect, it is first time to use the simple fluorescence spectrophotometer to determine the protein in human serum by red alizarin as the probe of the RLS. The experiment shows that the system HSA-red alizarin has the maximum intensity of the RLS in Britton-Robison buffer of pH4.10 at maximum wavelength of about 360nm. The method has better linear range within 0.040-10.0ug/mL, determination limit is 0.040ug/mL. Moreover, We have selected three other dyes of chromotrope 2R, acid chrome dark blue and pyrocatechin violet as the probes of the RLS in determining protein in human serum and achieving satisfactory results.

在生化分析方面，作者首次使用简易960型荧光分光光度计，利用茜素红作为共振光散射光谱探针对人血清中的蛋白质含量进行了测定，测定结果显示在pH4.10的Britton-Robinson缓冲溶液，波长360nm处出现最大的共振光散射强度，其线性范围为0.040～10.0μg／mL，检出限为0.040μg／mL；另外，作者还筛选了其他三种可用作蛋白质测定的共振光散射光谱探针的有机染料：变色酸2R、酸性铬深蓝、邻苯二酚紫，并深入研究了测定的适宜条件和干扰情况，将这三种有机染料用于人血清中蛋白质含量的测定，均取得了满意结果。

A simple and highly sensitive new kinetic catalytic fluorimetric method was proposed for the determination of trace chromium,based on the catalytic effect of trace amounts of chromium on the oxidation of Pyronine Y by hydrogen peroxide in acetic acid-sodium acetate buffer medium leading to a decrease in the fluorescence intensity.

在HAc-NaAc缓冲体系中，痕量铬对过氧化氢氧化吡咯红 Y的氧化还原反应有催化作用，使吡咯红 Y荧光减弱，据此建立了催化荧光法测定痕量铬的新方法，同时考察了该催化反应的最优条件及动力学性质。

Currently activities involve (1) Investigation of dye-DNA interaction by using spectroscopic methods to probe the binding sites between chromophore and DNA and to identify various DNA conformational structures, particularly the telomeric DNA;(2) Investigation of the structure-activity correlation of anti-telomerase agents;(3) Design and synthesis new compounds for antitumor agents and fluorescence biomarkers;(4) Monitoring the folding and unfolding of quadruplex structure, tracing the pathway of drug delivery and probing drug-target interaction by using single molecule microscopy and spectroscopy.

目前 的研究重点分别是(1)利用光谱方法探讨探针分子与DNA的交互作用，特别是作用位置与结构变化；(2)研究抗端粒酵素作用分子之结构与生物活性之关系；(3)设计及合成抗肿瘤药剂或萤光生物标记；(4)建立单分子萤光光谱与影像技术，研究 DNA的多变型结构之变化机制，监测药物输送的路径过程及其在细胞中与标的物的作用。

The method of quantum chemistry was used for studying the fluorescence spectra of three common pesticide compounds.

采用量子化学方法对几种农药化合物的荧光光谱进行了理论研究。

The results of the experiment were analysed with quantum physics and the theory of fluorescence bringing.

从量子物理和荧光产生的机理入手对实验结果进行理论分析。

It was found that SbW was a strong quencher of BSA fluorescence and the quenching was static.

荧光实验结果显示SbW与BSA通过静态猝灭形成1：1的SbW-BSA复合物，SbW的电化学线性扫描也证实了这种复合物的形成。

The results of the studies on fluorescence quenching by anionic quencher KI also confirmed the intercalative binding of PF with DNA.

阴离子KI的荧光猝灭实验进一步证实了酚藏花红与DNA之间的嵌入作用。

The difference of fluorescence quenching between light-adapted and dark-adapted bacteriorhodopsin showed this quencher can be used in the study of conformational change of Biomembrane proteins.

竹红菌乙素对明暗适应菌紫质荧光猝灭的差别，说明这种猝灭剂可用于生物膜体系的蛋白质构象变化的研究。

It was based on the quenching of the fluorescence of RVN in the presence of DNA.

DNA的存在使RVN的荧光猝灭，而且荧光猝灭的程度与加入DNA的量在一定的浓度范围内呈现良好的线性关系。

The maximal fluorescence intensity of the ring is proportional to the content of quinidine sulfate.An SOR method for the determination of quinidine sulfate was established in the range of 0.2 ~1.4×10~(-12) mol,and the limit of detection(3σ) was 2.6×10~(-14)mol.

研究表明，只要满足液滴与表面的接触角不为零、液滴与表面的接触线被固定、溶剂能挥发等3个条件，固载表面上液滴中的溶质将在固载表面的接触线上聚集而形成一个自组装成环。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。