查询词典 fluorescence

Results (1) The total RNA, reverse transcription product and fluorescence labeled cRNA were all of high quality;(2) There were 420 up-regulated genes and 552 down-regulated genes among 2-fold DEGs, including DKK1 (dickkopf homolog 1) which was 5-fold up-regulated;(3) The results of real time RT-PCR, using β-actin as an internal control, showed that the 2-ΔCt values of DKK1 in HCC, PCT and NLT were 0.089 504, 0.007 65 and 0.000 631 respectively.

结果 （1）配对组织总RNA质量高，反转录cRNA及荧光标记质量好；（2）2倍差异表达基因中，上调基因共420个，下调基因共552个，其中包括5倍上调基因DKK1；（3）以β-肌动蛋白为内对照的实时逆转录聚合酶链反应结果提示，DKK1在癌、癌旁和正常肝组织中的2-ΔCt值分别为0.089504、0.00765和

The effect of sleep improvement of the three dosages(570 mg/kg,1000 mg/kg and 2000 mg/kg)of Semen Ziziphi Spinosae was studied by the experiments of sleep-induced directly, prolonging sleeping time induced by pentobarbital sodium, affecting mouse sleeping rate under the threshold hypnogenesis dosage of pentobarbital sodium and affecting sleeping delitescence induced by tobarbital sodium. The content of 5-HT in the brain of mouse was measured by fluorescence assay.

通过直接睡眠实验、延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下催眠剂量实验和巴比妥钠睡眠潜伏期实验，比较低、中、高3个剂量（570 ，1 000 及2 000 mg/kg）酸枣仁粉剂对小鼠睡眠的影响，并且用荧光法测定小鼠脑中 5-羟色胺含量。

Triazine; dendron; thermo stability and solubility; energy transfer; fluorescence

1； 3； 5-三嗪；树枝块；热稳定性和溶解性；能量转移；荧光

The application based on the hybrid K-edge/ X-ray fluorescence densitometer instrument was studied.

利用现有混合式K边界/X荧光测量装置在Purex流程中进行了应用研究。

Methods Enamel surface specimens were randomly allocated into 3 groups: 1 450 mg/L sodium fluoride dentifrice group, 1 450 mg/L sodium monofluorophosphate dentifrice group and non-fluoride dentifrice control group. Sound enamel surfaces were dematerialized in bacteria model in vitro. QLF was used to analyze the area of lesionsArea, the loss of fluorescenceΔF and ΔQArea×ΔF on enamel surfaces after demineralization.

将30个牙釉质块开窗后分为3组，分别使用氟含量1 450 mg/L的氟化钠牙膏、单氟磷酸钠牙膏和无氟牙膏的处理液处理，并在体外恒化器龋病模型中进行脱矿循环，定量光导荧光技术QLF分析病损脱矿后的面积、荧光损失和总荧光损失量，激光扫描共聚焦显微镜CLSM分析脱矿后矿物质含量变化。

Methods Enamel surface specimens were randomly allocated into 3 groups: 1 450 mg/L sodium fluoride dentifrice group, 1 450 mg/L sodium monofluorophosphate dentifrice group and non-fluoride dentifrice control group. Sound enamel surfaces were dematerialized in bacteria model in vitro. QLF was used to analyze the area of lesions, the loss of fluorescence and ΔQ on enamel surfaces after demineralization.

将30个牙釉质块开窗后分为3组，分别使用氟含量1 450 mg/L的氟化钠牙膏、单氟磷酸钠牙膏和无氟牙膏的处理液处理，并在体外恒化器龋病模型中进行脱矿循环，定量光导荧光技术分析病损脱矿后的面积、荧光损失和总荧光损失量，激光扫描共聚焦显微镜分析脱矿后矿物质含量变化。

Enamel surface ecime were randomly allocated into 3 grou : 1 450 mg/L sodium fluoride dentifrice group, 1 450 mg/L sodium monofluoropho hate dentifrice group and non-fluoride dentifrice control group. Sound enamel surfaces were dematerialized in bacteria model in vitro. QLF was used to analyze the area of lesio, the lo of fluorescence and ΔQ on enamel surfaces after demineralization.

将30个牙釉质块开窗后分为3组，分别使用氟含量1 450 mg/L的氟化钠牙膏、单氟磷酸钠牙膏和无氟牙膏的处理液处理，并在体外恒化器龋病模型中进行脱矿循环，定量光导荧光技术分析病损脱矿后的面积、荧光损失和总荧光损失量，激光扫描共聚焦显微镜分析脱矿后矿物质含量变化。

The growth, the lymphocyte proliferation and the content of serum corticosterone were determined with specific growth rate, thiazolyl blue deoxidize reactionand fluorescence method, respectively.

用特定生长率的方法测定小鼠的生长，用噻唑蓝还原法测定淋巴细胞转化，用荧光法测定血清中皮质酮的含量。

Methods A total RNA from ten cases of ovarian cancer and contral normal ovarian tissues were extracted by a single step method. The cDNA retro-transcribed from equal quantity mRNA derived from ten cases of ovarian carcinoma and normal ovarian tissues, which were labeled cDNA strand with Cy5 and Cy3 fluorescence as probes.

按一步法分别抽提10例人卵巢癌组织和对照正常卵巢组织的总RNA并纯化mRNA；分别从10例取等量的人卵巢癌组织和对照组织的mRNA分别混合后逆转录合成以Cy5和Cy3标记的cDNA一链做探针，混合后在含有8464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

Methods The cDNA retro-transcribed from equal quantity total RNA derived from rectal carcinoma or normal rectal mucosa were labeled with Cy5 or Cy3 fluorescence as probes respectively. The mixed probes were hybridized with the BioStar 4096 cDNA microarray.

分别抽提直肠腺癌和正常直肠粘膜的总RNA；通过逆转录方法将Cy3和Cy5两种荧光分别标记到两组cDNA上，制成探针；利用这种cDNA探针与含有4096条人类基因PCR产物的表达谱芯片进行杂交，扫描芯片荧光信号图像，计算机分析后比较两种组织中差异表达的基因。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。