查询词典 fluorescence

It was found that the fluorescence spectra of the products of MMPB with GSH and with Cys both redshifted from λ〓/λ〓=299/355nm to λ〓/λ〓=305/425nm with the different rate. Under the following conditions: pH=7. 0 〓 buffer, T=35℃ and t=25min, the maximal fluorescence wavelengths were at λ〓/λ〓=299/355nm for MMPB-GSH and λ〓/λ〓=305/425nm for MMPB-Cys, respectively.

在pH=7.0的〓缓冲溶液中和35℃下，MMPB与GSH或Cys反应25min后，生成的MMPB-GSH的荧光峰为λ〓／λ〓=299／355nm，且此波长下的最大荧光强度可以稳定3h；而同样条件下，MMPB-Cys的荧光峰为λ〓／λ〓=305／425nm，且荧光较弱。

Near-infrared fluorescence of Yb was further enhanced while the visible fluorescence from Yb-Calcein was quenched when second ligand, such as tributyl phosphate, 1,10-Phenanthroline and trioctyl phosphine oxide, was added.

第二配体TBP，Phen，TOPO的加入，可进一步提高Yb~(3+)-钙黄绿素体系的近红外发光效率，但同时也会猝灭Yb~(3+)-钙黄绿素体系的可见区荧光。

The results of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L,there existed some stable hydrophobic regions,which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid,in the partially folded intermediate of Bacillus amyloliquefaciensα-amylase;with the denaturation concentration increasing,the stable hydrophobic regions disappered.the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed that using acrylamide as quenchers,with the protein denaturation extent increasing,the number of Trp that can be quenched increased untill all the Trp residues were quenched;Using potassium iodide as quenchers,with the maximum number(8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciensα-amylase molecule could be quenched by potassium iodide;with the denaturation concentration increasing,the number of Trp that can be quenched decreased to 5.the results of their protein electrophoreses and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciensα-amylase formed during the whole unfolding/refolding procedure of Bacillus amyloliquefaciensα-amylase induced by guanidine hydrochloride or urea.

ANS外源荧光探针结果表明：盐酸胍诱导的芽孢杆菌α-淀粉酶分子去折叠过程中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；而随着芽孢杆菌α-淀粉酶分子在盐酸胍溶液中变性程度的加深，这一疏水区域逐步被瓦解。丙烯酰胺和碘化钾猝灭结果表明：在盐酸胍溶液中，随着芽孢杆菌α-淀粉酶分子变性程度的进一步加深，其分子内能够被丙烯酰胺接近的色氨酸残基逐渐增多，直至全部被猝灭。但位于芽孢杆菌α-淀粉酶分子表面的能够被碘化钾猝灭的色氨酸残基，在中间态芽孢杆菌α-淀粉酶分子中数目达到最大的8个，而随着其分子变性程度的进一步加深，反而减少至5个。

In order to develop new DNA probes,we have synthesized two kinds of probes bipyrene ratiometric fluorescence probes and long wavelength Ruthenium polydiimine complex,the following work have been carried out:Fluorescent DNA probes with 1,6-hexanediyl as the linker between two pyrenes, phenylpyrenes or phenylethynyl pyrene fluorophores were synthesized(Py-1,Py-2 and Py-3) and their interactions with DNA were studied by UV-Vis absorption spectra,fluorescence spectra and viscosity measurements.

在前人的基础上，为了更好的识别DNA，本论文设计合成了两类DNA探针——双芘类比率型DNA荧光探针和长波长钌的配合物，具体如下：以己二胺为柔性链，以芘及其衍生物为发色团合成了探针Pyl-3，并通过荧光光谱，荧光紫外吸收光谱和黏度测试研究了探针与DNA的结合方式。

Low chlorophyll b rice mutant and the wild type rice (Zhenhui 249) were used to study the effects of low chlorophyll b on themiostability of photosystem Ⅱ in mutant by measuring their changes in chlorophyⅡ fluorescence parameters and polyphasic chlorophyⅡ fluorescence transients under different temperature treatments.

通过测定不同温度处理后水稻低叶绿素b突变体及其野生型（镇恢249）叶绿素荧光参数和快速荧光诱导动力学曲线的变化，研究了低叶绿素b对突变体光系统Ⅱ热稳定性的影响。

Studied the effect of the component, solvent and temperature on the fluorescence properties of these polyamides, the results showed that the fluorescence properties of these polyamides changed with the ratio of the substrate and other factors.

结果发现由于投料比及活化剂用量的不同，产物呈现出不同的荧光性能，这可能与不同条件下聚合物具有不同的文化结构有关。

The fluorescence is also a good measurement in identifying oil inclusions and an effective indicator of its maturity,except for some oil inclusions of lower homogenization temperature with white-blue fluorescence color which have been possibly caused by heating decomposition after trapping.

本文在论述有机包裹体荧光机理的基础上，依据不同盆地样品的实验观测结果，从油包裹体荧光的强度、波长、颜色以及与有机包裹体同期的盐水包裹体的均一温度等关系进行分析，证明可以运用油包裹体荧光颜色和平均均一温度等综合指示油气的成熟度

Fluorescence studies reveal that the self-coiling tendency of organic molecular driven by HLI strongly depends on the length of the chain and the donor and acceptor moieties linked by methylene chains approach each other because of the self-coiling to induce the fluorescence quenching and form the intramolecular exciplex or radical-ion pairs.

结果表明，在一定极性的溶剂体系中，具有一定碳链长度的有机分子在疏水亲脂作用的驱动下能够产生自卷现象，从而使长链两端的电子给体和电子受体相互靠近，发生电子转移，造成有机分子的荧光淬灭。

Methods and characteristics are described in this paper for fluorescence resonance energy transfer microscopy and fluorescence lifetime imaging microscopy.

本文叙述荧光共振能量转移显微术及荧光寿命成像显微术的原理、方法及特点。

The absorption spectrum and fluorescence of Ni2+-doped NASL glass and glass ceramics were measured. It shows that the Ni2+-doped NASL glass ceramics has a broad fluorescence spectrum centering at 1185 nm with full width at half maximum of about 300 nm.

测试了Ni2+掺杂NASL玻璃和微晶玻璃的吸收光谱和荧光光谱，发现Ni2+掺杂NASL微晶玻璃有较宽的荧光光谱，荧光中心位于1185 nm，荧光半峰全宽约为300 nm，荧光寿命和受激发射截面的乘积为4.2×10-24 cm2·s。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。