查询词典 fluorescence

The results show that ponceau 4R excited by light at the wavelength of 330-430 nm can generate a strong fluorescence at the 621 nm peak wavelength with its best excitation wavelength being 376 nm, amaranth excited by light at the wavelength of 300-440 nm can generate a strong fluorescence at the 643 nm peak wavelength with its best excitation wavelength being 370 nm, tartrazine excited by light at the wavelength of 280-380 nm can generate a strong fluorescence at the 565 nm peak wavelength with its best excitation wavelength being 315 nm, sunset yellow excited by light with wavelength of 310-410 nm can generate a strong fluorescence at the 592 nm peak wavelength with its best excitation wavelength being 348 nm, and brilliant blue excited by light at the wavelength of 320-390 nm can generate a strong fluorescence at the 456 nm peak wavelength with its best excitation wavelength being 350 nm.

结果表明，胭脂红在波长330～430 nm的光激发下，产生较强荧光，荧光峰值波长为621 nm，最佳激发波长为376 nm；苋菜红在波长300～440 nm的光激发下，产生较强荧光，荧光峰值波长为643 nm，最佳激发波长为370 nm；柠檬黄在波长280～380 nm的光激发下，产生很强荧光，荧光峰值波长为565 nm，最佳激发波长为315 nm；日落黄在波长310～410 nm的光激发下，产生较强荧光，荧光峰值波长为592 nm，最佳激发波长为348 nm；亮蓝在波长320～390 nm的光激发下，产生较强荧光，荧光峰值波长为456 nm，最佳激发波长为350 nm。

Secondly, we provide a two-level model to depict the one-, two-and three-photon fluorescent process and with it to calculate the one-, two-and three-photon fluorescence intensity dependence of the incident excitation intensity and time. We study the fluorescence intensity when excitation light's time distribution are square pulse、Gaussian pulse and sech pulse. We find that the influence of excitation light's time distribution on the two- and three- photon fluorescence peak intensity is larger than the one-photon fluorescence peak intensity. Two-level model can account for the behavior of saturation fluorescence.

然后用二能级模型解释了单、双、三光子激发原理，在理论上推导出了单、双、三光子吸收诱导荧光强度与脉冲强度以及脉冲作用时间的关系，并进一步研究了入射光时间分布分别为矩形、高斯型、双曲正割型时激发溶解在氯仿溶液中的C22H22N4O样品所产生的荧光峰值光强，结果表明激发光的时间分布对双、三光子荧光峰值强度的影响比对单光子的影响要大。

The results showed that: three major peaks,humuslike fluorescence peak A,proteinlike fluorescence peaks B and C appeared on the threedimensional fluorescence spectroscopy;the DOM fluorescence intensity was well related with DOC and CODMn,indicating that fluorescence dissolved organic matter content can reflect both the organic carbon content and the organic matter content by oxidation in the water in August,during the nonalgae bloom outbreak period,fluorescence intensity had a poor correlation with chlorophyll a,indicating that phytoplankton was not the main source of the fluorescent substances.

结果显示：小江回水区DOM的三维荧光光谱主要表现出3个峰，类腐殖质荧光峰A、类蛋白荧光峰B和C；DOM荧光强度与DOC、CODMn相关性较好，表明荧光溶解有机物质的含量可以较好反映水体中有机碳和可被氧化有机物的含量；在8月份非水华暴发时期荧光强度和叶绿素a相关性较差，表明DOM荧光物质主要不是由浮游植物产生。

To establish new methods for highly sensitive time-resolved fluorescence bioassay, several new lanthanide fluorescence complexes were synthesized and used for the preparations of nanometer-sized lanthanide fluorescence biolabels. Novel lanthanide fluorescence nano-biolabels are uniform (25-55 nm in diameters),strongly fluorescent with long fluorescence lifetime, highly photostable and biocompatible.

本项目以时间分辨荧光生物分析新方法的建立与应用为研究目的，在合成出几种稀土荧光配合物的基础上，建立了几种新型强荧光性纳米稀土荧光生物标记材料的可控制备方法，生物标记方法及其在时间分辨荧光免疫分析中的应用方法。

Objective To investigate the expression of intercellular adhesion molecular-1(ICAM-1) mRNA in contused skeletal muscle of rats using fluorescence in situ hybridization and its relationship with the contusion interval.

目的应用荧光原位杂交(fluorescence in situ hybridization，FISH)技术检测大鼠骨骼肌挫伤后细胞间黏附分子-1(intercellular adhesion molecular-1，ICAM-1)mRNA表达，分析其与挫伤时间的关系。

Co-firing characteristics of corn stalk and stone coal in different proportion were investigated by thermal gravimetric analysis. The results showed that corn stalk can improve the ignition and combustion of stone coal. The agglomeration experiments on corn stalk were made in a bench-scale bubbling fluidized bed with quartz sand as bed material and stone coal ash as additive. The results indicate that stone coal ash can inhibit agglomeration of bed material particles at temperature of 900℃. Agglomerate of blends was analyzed by means of scanning electron microscopy, energy-dispersive X-ray, and the bed materials were analyzed by means of X-ray fluorescence. Analysis showed that aluminum element and iron element in stone coal ash can react with alkali compounds and eutectics with low melting point, form a covering layer with high melting point on the surface of biomass and quart sand particles, thus inhibit the forming and transfer of low melting compounds.

以玉米秸秆与石煤按不同比例组成的混合物为研究对象，在TG-DTG热分析仪上进行了燃烧特性分析，结果表明玉米秸秆有利于石煤的着火和稳定燃烧，对石煤有一定的助燃作用；在小型鼓泡流化床实验装置上，以石英砂为床料、石煤灰为添加剂，进行了玉米秸秆成型燃料流化床燃烧的床料黏结实验，结果表明：石煤灰能够在生物质流态化燃烧过程中有效地抑制流化床床料黏结现象的发生；通过对实验中形成的结团进行扫描电子显微镜X射线能谱(scanning electron microscopy/Energy-dispersive X-ray- SEM/EDX)，对床料进行X射线荧光光谱(X-ray fluorescence，XRF)分析，结果表明石煤灰中的Al和Fe能够与生物质灰中的碱金属化合物以及低熔点共熔物发生化学反应生成高熔点物质，并且覆盖在生物质碳颗粒与石英砂颗粒表面形成隔绝层，从而阻止低熔点物质的生成与迁移。

Discovery of these microbial information in these unique ecosystems is rather encouraging for further novel species discovery, exploration and conservation of useful microbes. In the first section, the microbiota diversity of the former salterns was investigated by denaturing gradient gel electrophoresis and fluorescence in situ hybridization.

本研究第一部份先以变性梯度胶体电泳(Denaturing gradient gel electrophoresis，DGGE)针对微生物的16S rDNA片段进行分群，再以生物多样性指数加以描述，另辅以萤光原位杂交技术(Fluorescence in situ hybridization，FISH)调查本区域之微生物多样性。

Oocyte samples from one group were collected to detect the presence and integration of HBV DNA within cells and chromosomes using PCR, Southern blot, dot hybridization and fluorescence in situ hybridization. The female animals from another group were mated with their normal males, respectively. Their zygotes, 2-cell embryos were collected to detect the integration of HBV DNA in the female pronuclei of zygotes and the replication and expression of HBV genes in the 2-cell embryos using FISH, RT-PCR and immunofluoresence assay.(1) PCR detected positive bands in the tested oocyte samples fromgoldon hamster and mice. Southern blot revealed clear hybridization signals in PCR products.

研究用金黄地鼠和小鼠建立实验动物模型：将卵巢内注射HBV DNA的实验动物分成两组，一组注射后进行超排卵，收集卵巢和输卵管的卵母细胞，用PCR、Southern杂交，斑点杂交和荧光原位杂交(fluorescence in situ hybridization、FISH)检测HBV在卵母细胞内的存在和染色体上的整合；另一组超排雌鼠与正常雄鼠合笼，收集受精卵和2-细胞胚，用FISH、RT-PCR和免疫荧光检测技术分别研究HBV基因在受精卵雌原核上的整合以及在2-细胞胚中的复制与表达。

In the present study, we have used a biophysical approach based on Fluorescence Resonance Energy Transferto address these questions directly in living cells.

在本研究中，我们采用了一种生物物理的方法——荧光共振能量转移(Fluorescence Resonance Energy Transfer，FRET)，在活细胞中直接研究ASICs的装配情况。

Fluorescence resonance energy transfer is increasingly used to study inter-and intra-molecular interactions in living cells.

荧光共振能量转移(fluorescence resonance energy transfer ，FRET)技术日益广泛的应用于检测活细胞中分子内和分子间的相互作用。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。