查询词典 fluorescence

In present study, we discussed the effects of cell microfilament on the activity of collagen type I alpha 1 chain gene (COL1A1) promoter under microgravity simulated by clinostat and/or cytochalasin B as microfilament depolymerizer in the established EGFP-ROS cell line using the method of fluorescence semi-quantitative analysis and the fluorescent stain of microfilament.

但微重力对相关基因调控的影响知之甚少，本研究以构建的基因工程细胞株为对象，以回转器模拟微重力效应，利用增强型绿色荧光蛋白（enhanced green fluorescence protein，EGFP）荧光半定量和细胞微丝荧光染色分析技术，探讨回转模拟微重力条件下，细胞微丝系统对I型胶原α1链基因（collagen type I alpha chain 1，COL1A1）启动子活性的影响。

In present study, we discussed the effects of cell microfilament on the activity of collagen type I alpha 1 chain gene ( COL1A1) promoter under microgravity simulated by clinostat and/or cytochalasin B as microfilament depolymerizer in the established EGFP-ROS cell line using the method of fluorescence semi-quantitative analysis and the fluorescent stain of microfilament.

本研究以构建的基因工程细胞株为对象，以回转器模拟微重力效应，利用增强型绿色荧光蛋白(enhanced green fluorescence protein，EGFP)荧光半定量和细胞微丝荧光染色分析技术，探讨回转模拟微重力条件下，细胞微丝系统对I型胶原a1链基因(collagen type I alpha chain 1 gene， COL1A1)启动子活性的影响。

Significant Spearman rank correlations were found between the results obtained by Luminex assay and by chemiluminescence immunoassay or time-resolved fluorescence immunoassay. Only 1 microliter of serum and 3 hours were needed for Luminex assay. With the advantages of unique capability of multiplexing and high-throughput, good reproducibility, increased detection range and sensitivity, Luminex assay would have a great potential in clinical application.

本研究应用液相芯片技术利用双抗体夹心免疫分析原理同时检测了人血清中的癌胚抗原、甲胎蛋白和乙肝表面抗原，评价了该方法的线性范围、最低检测限、精密度、灵敏度、特异度、准确度等指标，并对检测结果与化学发光免疫分析法（chemiluminescence immunoassay，CLIA）和时间分辨荧光免疫分析法（time-resolved fluorescence immunoassay，TRFIA）进行了相关性分析。

In the present study, soil samples were scanned by NITON XLt920 field portable X-ray fluorescenceanalyzer, and the relationship between the X-ray fluorescence spectra and the concentration of Pb in soil was studied.

在实验室条件下，利用NITON XLt920型便携式X射线荧光光谱（field portable X-ray fluorescence， FPXRF）仪获取土壤样品的X射线荧光光谱数据，并采用偏最小二乘法建立土壤Pb含量的预测模型。

Culture and laboratory tests, including fluorescence-labeled antibody staining, PCR of virulence factor genes and Ascoli precipitin test on tissue specimens, identified the organism as Bacillus anthracis.

组织检体进行细菌培养及其他实验室检查，包括FA染色(fluorescence-labeled antibody staining)、毒力因子基因PCR及Ascoli precipitin test，证实病原为炭疽杆菌。

Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法：成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒，手术后雄鼠与超排雌鼠合笼交配，用荧光原位杂变(Fluorescence in situ hybridization，FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制，用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction，RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

Here, we used fluorenscence detection methods including bimolecular fluorescence complementation and Fluorescence Resonance Energy Transfer to address these issues.

我们采用荧光检测法包括双分子荧光互补法(bimolecular fluorescence complementation BiFC)和荧光共振能量转移法(Fluorescence Resonance Energy Transfer FRET)。

The novel restorer line Nsa restorer-1 and its progenies were analysed by genomic in situ hybridization combined with double-colour fluorescence in situ hybridization technologies, the results showed that Nsa restorer-1 was Brassica napus-Xinjiang wild Sinapis arvensis disomic alien addition line,consisting of the whole genome of Brassica napus and a homologous chromosome pair of Xinjiang wild Sinapis arvensis.

用基因组原位杂交(genomic in situ hybridization， GISH)结合双色荧光原位杂交(double-colour fluorescence in situ hybridization， dc-FISH)技术，对甘蓝型油菜新型恢复系Nsa恢1及其后代进行了研究，结果表明，Nsa恢1为甘蓝型油菜-新疆野生油菜二体异附加系，包含了甘蓝型油菜全基因组和新疆野生油菜1对染色体，这1对新疆野生油菜染色体为同源染色体。

Objective To study the technique and diagnostic value of fluorescence insitu hybridization in chromosome abnormality for prenatal diagnosis. Methods Amniocenteses were performed in 34 pregnant women of 16～23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium.

荧光原位杂交(fluorescence in situ hybridization， FISH)是分子杂交与组织化学相结合的一项新技术，近年来用于人类染色体分析，它能快速、准确地确定在传统方法中无法判别的染色体异常，特别对来源不明的标记染色体能作出正确诊断［1，2］。

To investigate the relationship between delayed fluorescence change and chlorophyll content of Gerbera hybrida ray floret during its development (from P1 period to P6 period), delayed fluorescence analysis of Gerbera hybrida ray floret during its development was done.

以非洲菊Gerbera hybrida为研究对象，对其生长发育过程（P1-P6期）中外轮舌状花进行延迟荧光（delayed fluorescence， DF）分析，并利用激光共聚焦成像系统对其各期表皮细胞中叶绿素的分布和相对含量进行检测，以研究非洲菊外轮舌状花发育过程中延迟荧光改变与其叶绿素变化的关系。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。