查询词典 flow line

MethodsThe cells of human gastric cancer cell line SGC7901 was treated respectively with Chrysin at different concentration 10、20、40、80μM for 48h. The proliferation inhibitory rate was measured by MTT assay. Acridine orangestaining and flow cytometry were used to detect apoptosis. Western blot assay was used to detect the expression of apoptosis related genes NF拨B,Bcl2,Bax.

方法分别用10、20、40、80μM的ChR处理人胃癌细胞株SGC7901 48h，采用MTT比色法检测ChR对SGC7901细胞的增殖抑制效应；采用丫啶橙染色、流式细胞术检测ChR诱导SGC7901细胞凋亡的发生；应用Westernblot法检测凋亡相关基因NF拨B、Bcl2、Bax的蛋白表达，并用计算机图像分析软件进行半定量分析。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

ANSYS is a finite element simulation software used to simulate the temperature field in selective laser sintering.By using the ANSYS parameter design language,the density of the thermal flow,the velocity and the path of scanning can be controlled.The effect of the scanning path on the temperature field in the SLS process is studied. Balling,which occurs at the end of the scanning line,is caused by the dissymmetric temperature field and the great temperature gradient.

利用有限元分析软件AN SY S，对激光烧结温度场进行了数值模拟，建立了激光烧结薄板的温度模型；运用APDL语言控制热源的热流密度、移动速度以及扫描轨迹；研究了激光行程对烧结温度场的影响，扫描端点温度场的不对称及较大的温度梯度造成了端点球化现象；激光扫描线间的耦合作用使端点球化现象随扫描线的增加而逐渐显著。

Then, interruptible load is introduced into transmission congestion management in the paper. The paper discusses the application of interruptible load to transmission congestion management in power pool market. Market risk of evade is very important in transmission congestion management. The FGR, FTR, TO are introduced into transmission congestion management, its concept and implement in bilateral market are discussed in the paper. It provides the valid means of evadable price risk for the market participant. The reasonable allocation of congestion cost is very important in transmission congestion management. The paper proposed a novel methodology for allocation of congestion cost based on the line flow analysis.

然后，将可中断负荷引入到输电阻塞管理中，对其在电力联营体交易市场模式下输电阻塞管理中的应用进行了全面阐述；市场风险的规避是输电阻塞管理中需要考虑的重要内容，本文将基于潮流的输电权、金融型输电权及输电期权引入到输电阻塞管理中，阐述了其基本概念以及在双边交易市场模式下的具体实施，为市场参与者提供了规避价格风险的有效手段；阻塞费用的合理分摊是输电阻塞管理中的重要内容，本文提出了一种基于阻塞线路潮流组成分析的阻塞费用分摊新方法。

Through the comparison and study of risk issue and monitoring measurements of second generation of biotech crops, the author provided the risk management and monitoring measurements to PMPs: develop the regulation to safety assessment of PMPs; set other bioreactor as control when evaluate the intended use; set the non-transgenic iso-line as control when evaluate the food and environment safety; design the product without activity during production of PMPs; using GURT to prevent the gene flow.

通过比较分析国际上对未来新一代转基因作物，尤其是药用转基因作物风险评价与管理对策研究，提出我国对未来药用转基因作物评价与监管的建议：完善药用转基因作物评价规定；对预期使用的评价要以其他生物反应器为对照进行评价；对食用安全与环境安全评价要以其非转基因对照比较；设计产品生产过程中无活性，加工(来源：Adf3fBC论文网www.abclunwen.com)工程中激活；利用遗传限制技术使药用植物花粉不育等。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

The imaging confirmed blood flow shutdown in a wide variety of advanced imageable malignancies, safety is in line with expectations and tumor responses were seen in multiple patients.

所成图像证实阻止了大多数种类的晚期成像恶性肿瘤的血液流动，安全性符合预期，多名患者表现出产生肿瘤反应。

Pentek filter systems offer multistage, reverse osmosis, in-line, countertop, shower and faucet filter options, while Pentek specialty products and processes such as ultraviolet, bromination, iodination, and radial flow iron reduction increase our ability to meet specialized needs.

pentek过滤系统提供了多级，反渗透，在网上， countertop ，淋浴和水龙头过滤选项，而pentek特种产品和新工艺，如紫外线，溴化，碘化，并径向流还原铁增加我们的能力，以满足特殊需求。

In agriculture, introduced a total investment of 60,000,000 yuan Zhangjiajie in Hong Kong and Vietnam's pig production and processing projects with a total investment of 12,000,000 yuan in Guangxi oak, Jiang Yun-Rosin Rosin's line item; in the flow of commerce in the area, the introduction of a supermarket Fung Choi, Maney Supermarket, High Tech Computer City furniture and other large-scale supermarkets, 6; in the development of urban construction, the completion of the He Long Sports Center, Division race, Kim Garden, Kangxi Trade City, pedestrian film, the natural gas pipeline network, such as a large number of investment projects Building.

在农业方面，引进了总投资6000万元张家界港越公司乳猪生产加工项目，总投资1200万元广西橡州运江松香公司松香生产线项目；在商贸流通领域，引进了丰彩超市、梅尼超市、宏达家私城等各类大型超市6家；在城市建设开发方面，完成了贺龙体育馆、科赛、金豪花园、康茜商贸城、影视步行街、天然气管网等一大批招商项目的建设。

Methods: Using the techniques of cell culture ex vivo, flow-cytometry, immunocytochemistry, the effects of GPS on the proliferation of human promyelocytic leukemia cell line(HL-60) and human granulocytopoiesis and monocytopoiesis were observed.

结果：GPS在体外能抑制HL-60细胞增殖，而同等剂量的GPS能明显促进正常粒单系造血祖细胞的增殖分化；在本实验条件下未见GPS对HL-60细胞有明显的诱导分化作用。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。