查询词典 flow into

The full-leng coding sequence of ALK1 was cloned into pcDNA3.l+using standard protocols. The constructed pcDNA3.1+ALK1 plasmid were transfected into HUVECs. The proliferation and migration of HUVECs were detected by boyden champer and flow cytometry.

脂质体转染pcDNA3.1+ALK1到HUVECs，流式细胞仪检测HUVECs增殖的改变，boyden小室检测ALK1对HUVECs迁徙的影响。

Then, interruptible load is introduced into transmission congestion management in the paper. The paper discusses the application of interruptible load to transmission congestion management in power pool market. Market risk of evade is very important in transmission congestion management. The FGR, FTR, TO are introduced into transmission congestion management, its concept and implement in bilateral market are discussed in the paper. It provides the valid means of evadable price risk for the market participant. The reasonable allocation of congestion cost is very important in transmission congestion management. The paper proposed a novel methodology for allocation of congestion cost based on the line flow analysis.

然后，将可中断负荷引入到输电阻塞管理中，对其在电力联营体交易市场模式下输电阻塞管理中的应用进行了全面阐述；市场风险的规避是输电阻塞管理中需要考虑的重要内容，本文将基于潮流的输电权、金融型输电权及输电期权引入到输电阻塞管理中，阐述了其基本概念以及在双边交易市场模式下的具体实施，为市场参与者提供了规避价格风险的有效手段；阻塞费用的合理分摊是输电阻塞管理中的重要内容，本文提出了一种基于阻塞线路潮流组成分析的阻塞费用分摊新方法。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

In addition, as the secondary steam get in direct contact with the ejection flow to carry out heat exchange, most steam is condensed into water, while the uncondensed small minority steam and the uncondensed gas will also get into friction, with the high speed water ejection, mixed and extruded, then discharged through diffusing pipe, thus to form up higher vacuum inside.

另外由于二次蒸汽与喷射水流直接接触，进行热交换，绝大部分的蒸汽凝结成水，小量未被冷凝的蒸汽与不凝结的气体亦由于与高速喷射水流互相摩擦，混合挤压，通过扩压管被排除，使器室内形成更高的真空。

The only option to increase the flow of negative nodes into the system is to move vertically, or deeper into the disciplines that underly the vendor and startup sports that attract most of the raw attention. It is here that the Open Web Foundation appears on the scene, a honeypot for attracting negative gesturers.

或许真正的自我应该是建立在自我的意志之上的，人与人之间的彼此的熟悉也是要建立在自我的意志之上的，有时候，表面的东西只会蒙蔽彼此的眼睛，甚至有的时候我们亲眼看到的亲耳听到的也未必是事实，所以真正了解一个人，必须深入地接触，短暂地接触并不可能真正的了解，对于任何人都一样，对于那些只有很少接触的人，应该很少给予评价，因为我们还不够了解，并且没有那个权利去评价，每个人都有自己的是非观和人生观，只是表现的形式不同，但无论你的是非观什么怎么样的，到最后还是逐渐会被同化，也就是说会被社会的现实所慢慢改变，这使我感到悲哀，所以我一直对自己说并经常给我身边的朋友说这么几句话：同流不合污，随波不逐流；改变能改变的，接受无法改变的。

Cut into short lengths and cooled by turbulent air flow, these microfibers are then formed into a complex weblike structure on a moving conveyor screen.

它不同于纱线的形态，而是被切断的短纤维，通过气流冷却并铺散到一条传输带上，成为复杂的网络结构。

It only took 38 months from construction preparation at the end of October, 2001 to the first unit operating on December 20, 2004, which antedated the construction time limit by 7 months. During the period, 5-month-construction period delayed was saved because of the karst geological problems of dam's partial foundation. 3 units were put into operation in 2005, especially it only took 23 days from the runner of 4# unit hoisted to put into operation. It created the wonder of installing large axial-flow feathering unit.

乐滩电站从2001年10月底开始施工准备到2004年12月20日首台机组投产发电，仅用38个月的时间，比合理工期提前了7个月，并在此期间，抢回因厂坝部份地基岩溶地质问题延误的5个月工期。2005年又实现了年内3台机组投产发电，尤其4号机组从转轮吊装到机组并网发电，仅用23天，创造了大型轴流转桨机组安装奇迹。

The Yangtse River learns unity and coherence in writing academy more than 40 names students enthusiastically in the afternoon on October 24, autumn loafs about on precious Ta He county of neighbouring the Yangtse River Guanyin rock projecting over the water Sha Shi Duan sandy beach cooking a meal in the open. Shi more than 10 college student present is carried out immediately saved , one important more than ten students saw that the power is composed of the person ladder offal at that time rescuing people , still had a sum daughter schoolmate who can not swim for among them , thought that second children save quickly when going on shore in 14:15 or so , the incautious drop-in of 2 vicinity children river,the ladder has fallen apart , nine college students have fallen into water since the lack of physical strength prepares the subterranean flow , person, rescue hubbub slice immediately , happen to make contact with winter swimming sixty several old people the coming year rescue up six college students group, Two fall into water teenager has been rescued, but three heroes indistinct but ice-cold river water of submergence is already middle.

10月24日下午，长江大学文理学院40多名学生，在长江观音矶附近的沙市宝塔河段沙滩上秋游野炊。14时15分左右，附近2名儿童不慎落入江中，在场的10余名大学生立即开展施救，当时十几名大一学生见势组成人梯下水救人，其中还有不会游泳的和女同学，当第二个小孩快救上岸时，由于体力不支和暗流，人梯散了，九名大学生落水，顿时救喊声一片，正好碰上冬泳队几名六十来岁的老人救起六名大学生，两位落水少年获救了，而三位英雄已经沉入苍茫而冰冷的江水中。

Torrent hit reefs, crystal flower fly, waves roar,Jiang chaos cover flow, there is a half down the middle , People's Bank, the springs gush from the top of the head and, woven into a marvelous hanging peg the , Journey Into Amazing Caves.

激流撞礁，晶花腾空，惊涛轰鸣，如雷贯耳。盖江崖头乱泉涌流，拦腰有一条半里磴道，人行当中，泉水从头顶喷泻而，织成一挂挂奇丽的珠帘，别有洞天。

Weaker wave action in the estuary area, the river for several groups of bifurcation into the sea at the same time, the cross-flow of silt accumulation in both the volume of more than wave erosion, silt accumulation along the Fork Road extension to form a long strip big spit into the sea, so that was a bird foot delta-shaped appearance.

在波浪作用较弱的河口区，河流分叉为几股同时入海，各叉流的泥沙堆积量均超过波浪的侵蚀量，泥沙沿各叉道堆积延伸，形成长条形大沙嘴伸入海中，使三角洲外形呈鸟足状。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。