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In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

Commodity flow, money flow and income flow, it is 3 the most primary technological process in goods economy, it is a whole that has immanent connection.

商品流程、货币流程和收入流程，是商品经济中最基本的三个流程，是一个有内在联系的整体。

Main creative points of the thesis are as following:1. It is the first time to apply 2-D and 3-D turbulent model of the air-water two phases flow to the study on hydraulic properties of groyne. It makes description of the turbulent flow field to be possible precisely. This offers a more accurate method to determine the water surface in channel improvement.2. Based on the results of experiment and simulation for 3-D turbulent model of air-water two phases flow, a new formula to compute the height of damming is built.3. Based on the 2-D mode and the formula to compute the height of damming, the subdivision method of 2-D computation for water surface at upstream and downstream.

本文的创新工作主要是：首次将三维水气两相流紊流数学模型应用于丁坝水动力学特性研究，使丁坝附近流场和紊动场的精细描述成为可能，为航道整治中水面线确定提供了一个更为准确的方法；(2)基于水槽实验和三维水气两相流紊流数学模型数值模拟结果，建立了新的丁坝前奎水高度计算公式；(3)基于上述奎水高度计算公式和本文二维水面计算数学模型，首次提出了丁坝上下游二维水面分段计算方法。

The bifurcated flow, a common morphology of flow motion, usually occurs in natural rivers and headrace open channels etc. Controlled by its boundary, the bifurcated flow is complicated in structure and specialized in river evolution.

分汊流是常见的水流运动形态，常出现在天然河流、引水明渠等工程中，受边界控制，水流结构复杂，河道演变特殊。

I love the amniotic fluid when it spills out of the bag. I love the kidney with its painful gallstones, its gravel and whatnot; I love the urine that pours out scalding and the clap that runs endlessly; I love the words of hysterics and the sentences that flow on like dysentery and mirror all the sick images of the soul; I love the great rivers like the Amazon and the Orinoco, where crazy men like Moravagine float on through dream and legend in an open boat and drown in the blind mouths of the river. I love everything that flows, even the menstrual flow that carries away the seed unfecund. I love scripts that flow, be they hieratic, esoteric, perverse, polymorph, or unilateral. I love everything that flows, everything that has time in it and becoming, that brings us back to the beginning where there is never end: the violence of the prophets, the obscenity that is ecstasy, the wisdom of the fanatic, the priest with his rubber litany, the foul words of the whore, the spittle that floats away in the gutter, the milk of the breast and the bitter honey that pours from the womb, all that is fluid, melting, dissolute and dissolvent, all the pus and dirt that in flowing is purified, that loses its sense of origin, that makes the great circuit toward death and dissolution.

我爱从羊膜中溅出的羊水；我爱生着引起痛苦的的结石、肾砂和诸如此类东西的肾脏；我爱撒出的热呼呼的尿和久治不愈的淋病；我爱歇斯底里的疯话、像拉痢疾一样一泻而出的句子和灵魂全部病态的映像；我爱亚马逊河和奥里诺科河这样的大河，那儿摩拉瓦基乃之流的狂人在一只无顶的小船上漂过了梦和古老的传说，淹死在瞎眼的河口中；我爱流动的一切，甚至爱女人来月经时流出的血，它冲走了生育能力不强的精子；我爱会流动的手稿，不论它们是用象形文字写的、深奥的、反常的、多形体的或是单边音的；我爱流动的一切，一切其中有时间的和适当的东西，它们把我们带回永远不会结束的开始中，即先知们激烈、令人狂喜的猥亵，宗教狂的智慧，牧师和他的橡皮连祷文，妓女的下流话，从排水道里漂走的唾液，乳房里的奶汁和子宫里流出的带苦味的蜜水，以及一切流质的、溶化的、放荡的和有溶解力的，所有在流动中得到静化的脓和脏物，那些失去其出身意识的东西和那些将大循环驱向死亡和瓦解的东西。

I love the amniotic fluid when it spills out of the bag. I love the kidney with its painful gallstones, its gravel and what not; I love the urine that pours out scalding and the clap that runs endlessly; I love the words of hysterics and the sentences that flow on like dysentery and mirror all the sick images of the soul; I love the great rivers like the Amazon and the Orinoco, where crazy men like Moravagine float on through dream and legend in an open boat and drown in the blind mouths of the river. I love everything that flows, even the menstrual flow that carries away the seed unfecund. I love scripts that flow, be they hieratic, esoteric, perverse, polymorph, or unilateral. I love everything that flows, everything that has time in it and becoming, that brings us back to the beginning where there is never end: the violence of the prophets, the obscenity that is ecstasy, the wisdom of the fanatic, the priest with his rubber litany, the foul words of the whore, the spittle that floats away in the gutter, the milk of the breast and the bitter honey that pours from the womb, all that is fluid, melting, dissolute and dissolvent, all the pus and dirt that in flowing is purified, that loses its sense of origin, that makes the great circuit toward death and dissolution.

我爱从羊膜中溅出的羊水；我爱生着引起痛苦的的结石、肾砂和诸如此类东西的肾脏；我爱撒出的热呼呼的尿和久治不愈的淋病；我爱歇斯底里的疯话、像拉痢疾一样一泻而出的句子和灵魂全部病态的映像；我爱亚马逊河和奥里诺科河这样的大河，那儿摩拉瓦基乃之流的狂人在一只无顶的小船上漂过了梦和古老的传说，淹死在瞎眼的河口中；我爱流动的一切，甚至爱女人来月经时流出的血，它冲走了生育能力不强的精子；我爱会流动的手稿，不论它们是用象形文字写的、深奥的、反常的、多形体的或是单边音的；我爱流动的一切，一切其中有时间的和适当的东西，它们把我们带回永远不会结束的开始中，即先知们激烈、令人狂喜的猥亵，宗教狂的智慧，牧师和他的橡皮连祷文，妓女的下流话，从排水道里漂走的唾液，***里的奶汁和子宫里流出的带苦味的蜜水，以及一切流质的、溶化的、放荡的和有溶解力的，所有在流动中得到静化的脓和脏物，那些失去其出身意识的东西和那些将大循环驱向死亡和瓦解的东西。

In a steady flow experiment,measurements and data processing of 3-D flow velocity are made by hot-wire probe and flow visualization techniques.

作者在稳流实验中应用热线风速仪普通探针及流动可视化法完成了三维速度的测量及数据处理。

The characteristic of which thermal air flow sensor can directly measure air mass flow is used,a hot-wire air flow sensor which can be applied to intake system for an internal combustion engine is designed.

利用热式空气流量传感器直接测量空气质量流量的特点，设计了一种应用于测量内燃机进气系统进气量的热线式空气流量传感器。

In order to overcome the weaknesses in the traditional methods for base flow separation, such as the lack of both physical basis and feasible procedures, three basic principles for base flow hydrograph separation were proposed, based on which a new base flow hydrograph separation method was developed.

针对现行流量过程线分割方法中存在著缺乏物理基础和客观的、科学的操作方法，以及其他的一些问题，提出了流量过程线的分割应遵循的三条基本原则，在此基础上提出流量过程线分割的新方法。

Acoustic Doppler Current Profilers is a new kind of flow measuring device widely used in hydrometry. It uses the principle of Acoustic Doppler instruments to measure flow velocity profile. A short time flow measurement, high resolution, good accuracy, integrity data and great information are the characters of ADCP.

多普勒测流仪是当前在水文行业推广的一种新型测流仪器，该仪器利用声学多普勒原理，测量水流速度剖面，具有测验时间短、分辨率高、精度好、资料完整、信息量大等优点。

After the comparison to the two methods, the production of chitosan preparedby acid hydrolysis was confirmed considering to the safety of chitosan.

比较两种方法的优劣，为保持壳聚糖的安全性，确定了酸法降解壳聚糖的生产工艺。

I imagine her with all the badass angels now.

想象着他在天堂里和一群美丽的天使在一起的情景，她感到很很妒忌。