查询词典 flat bottom flask

The stirrer head should be near the bottom of the flask.

搅拌器的搅拌头要尽可能接近瓶底，但不要直接接触。

Methods: The three lung cancer cell lines-A549(highly invasive human lung adenocarcinoma cell ), 95-D(highly metastatic human lung carcinoma cell), and SPC-A-1(lowly metastatic human lung adenocarcinoma cell) were cultured conventionally in vitro. When the cells covered the bottom of culture flask, we abstracted the protein with lysis solution containing RIPA and PMSF, and obtained RNA with RNeasy Mini Kit.

将三株肺癌细胞：高侵袭的肺腺癌细胞(A549)、高转移的肺癌细胞(95-D)、低转移的肺腺癌细胞(SPC-A-1)做常规细胞培养，待细胞长满瓶底后，用RIPA和PMSF组成的细胞裂解液提取细胞蛋白质，RNeasy Mini Ki提取RNA。

The size of round bottom flask, temperature and water addition were the key of volatile oil extraction of C. uncinata, and addition of N-hexane was helpful for the extraction and collection of volatile oil.

圆底烧瓶的大小、温度及加水量是柱果铁线莲挥发油提取的关键，而添加正己烷有利于挥发油的提取与收集。

The second passage was obtained after the cell overgrew the bottom of the culture flask and purified by trypsinization and adherence repeatedly methods.

方法1。用组织块贴壁培养法获得原代卵巢癌CAFs和卵巢NFs，细胞铺满培养瓶后首次传代，通过胰酶消化法和反复传代法进行细胞的纯化；2。

Filter the cold solution using the Hirsch funnel (cover the bottom with a wet 17 mm filter paper) into a 50 ml filtering flask (don't forget to attach the neoprene filter adapter).

用赫氏漏斗(把一张湿的17 mm长的滤纸覆盖在它的底部)过滤冻的溶液，用一个50 ml的抽滤瓶接收(不要忘记要加一个由氯丁橡胶制的过滤接头)。

Clean the bottom of the trench for the ground plate so that there is a 10-foot long flat bottom at a depth of about 36 inches.

Oslash；清理干净沟槽的底部，得到一个10英尺长的平底36英寸深的安装接地铜片的地方。

Therelationship between the turbulent kinetic energy and its dissipation rate,whichis widely used to parameterize the dissipation rate in turbulence closure models,is found to hold well for both reversing and rotating flows,but with differentcoefficients.Microstructure profiling measurements at two comparative stations (a deepercentral basin and a local shelf break) in the stratified Yellow Sea are analyzed,with emphasis on tidal and internal-wave induced turbulence near the bottomand in the pyenocline.The water column has a distinct three-layer thermohaline structure,consisting of weakly stratified surface and bottom boundary layers anda narrow sharp pycnocline.Turbulence in the surface layer is controlled by thediurnal cycle of buoyancy flux and wind forcing at the sea surface.while thebottom stress induced by barotropic tidal eurrents dominates turbulence in thebottom boundary layer.The maximum level at which the tidally enhanced mixingcan affect generally depends on the magnitude of the tidal current,and it canbe up to 10-15 m in the Yellow Sea.This suggests that,in the deeper regionsof the shelf seas,turbulent dissipation and mixing are very weak at the levelsbetween the near-bottom tidally enhanced layer and the pycnocline.Therefore,these levels provide a significant bottle neck for the vertical exchanges.In theshallow regions,however,the tidally-induced turbulence can occupy the wholewater colum below the pycnocline.A quarter-diurnal periodicities of the turbulentdissipation rate and eddy diffusivity are found at different heights with evidenttime lag.In the relatively flat central basin,the pycnocline is essentially non-turbulent and internal-wave activity is very weak.Therefore,vertical fluxes acrossthe pycnocline decreased to molecular levels.In contrast,internal waves of variousperiods can be always found near the local shelf break.

对强层化季节黄海两对比性站位(分别位于中央海盆区与局地陆坡区)处层化、内波以及湍流混合特征的研究结果表明：1、强层化季节的陆架海水体一般呈现显著的三层热盐结构，在水体近乎混合均匀的上混合层与潮流底边界层之间为强跃层；2、近表层水体的湍流混合强度主要由海表浮力通量的日变化与海表风强迫控制，而在潮流底边界层内，潮混合是水体热量、物质、动量与能量垂直交换的主要机制；3、潮混合影响的深度由潮流大小决定，在黄海，一般可达10-15 m，因此，在水深较深的区域，在跃层与潮混合所至深度范围的上界之间存在湍流混合非常弱的区域，这显著抑制水体内物质的垂直通量，为物质垂直交换的瓶颈，而在水深较浅的区域，潮混合影响范围可至跃层底部，因此物质在跃层以下整个水体中混合非常均匀，当跃层内间歇性强混合发生时，可以产生显著的跨跃层物质输运；4、近底潮致强湍流耗散缓慢地向上传播，底上不同深度处垂直湍扩散系数也具有显著的位相差异，且二者均随时间呈现四分之一周日周期的变化；5、在地形较为平坦的中央海盆区，内波活动非常微弱，因此跃层内湍流混合非常弱，垂直扩散系数为分子扩散水平，跨跃层物质通量受到显著抑制，而在地形变化较为显著的局地陆坡区，内波活动非常活跃，除内潮的影响外，高频内波与内孤立波的影响也很显著，因此跃层内存在很强的间歇性强混合，内孤立波存在的区域，水体湍流混合显著增强。

The bottom frustum and the top face shall be scabbled to flat. The bottom face shall be no less than 60% of the top face.

底面截锥体、顶面应粗琢平整，底面积不应小于顶面积的60%。

The rolling--ball flat--bottom mill shoe whose bottom face is overlaid with YD alloy is used to fully grind junks which is stuck downhole and impossible to be caught. Additionally, rolling balls which are dressed on cylinder face of mill shoe can guarantee the mill shoe not to mill casing inside wall during milling operation in hole.

滚球式平底磨鞋是用底面所堆焊的YD合金去全面磨碎那些卡死在井内无法打捞的钢铁落物，并且在磨鞋圆柱面上有可以起扶正作用的滚球，使磨鞋在井眼内工作时不磨铣套管内壁。

The flat bottom and the right-angle rims of the mouth, the bottom and the ears form a pleasant contrast with the curves of the splay ears, the hoof-shaped feet, and the contracted belly, endowing the form of the cauldron with rhythm.

腹底由圆而变平，方折的口沿、底沿和耳沿，与外撇耳、蹄形足和内收的鼎腹等部位的圆弧线形成对比，刚柔相济，使得鼎的造型赋于节奏和韵律。

He paid $5,000 for this cupcake.

他我这个纸杯蛋糕花了5000美元。

I mean, if you want to, that is

我的意思是，如果你们想吃的话。