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Factors involved in the expression of IFN -αwere studied. These included multiplicity of infection, cell inoculation concertration and cell growth phase. The results show that lower mulitiplicity of infection (M01.4PFU/cell) is beneficial to IFN-α production.The optimal cell inoculation concentration was 〓 cells/flask. The high efficient replication of BmNPV-IFN-α and expression were also due to the proper inoculation time of BmNPV-IFN-α, which was at 48-60 house after cell passage.

研究结果表明，重组病毒感染复数的改变对α-干扰素的产量的影响是时限性的，在一定低感染复数时（Mo1.4PFU），α-干扰素的产量较高；对于重组病毒的复制，最适的细胞接种密度为3.6～7.0×〓细胞/瓶；在细胞指数生长前期（48～60小时）接种重组病毒，对于获得较高的重组病毒效价和α-干扰素的产量都是有利的。

How to select the absorbing liquid for the oxygen flask combustion method

如何选择氧瓶燃烧法的吸收溶液？

Methods Oxygen flask combustion method was adopted.

采用氧瓶燃烧法对氧氟沙星进行含量测定。

a simple and accurate method is established for determination of ciprofloxacin in oxygen flask combustion method

氧瓶燃烧法定量准确、灵敏度高，可作为测定环丙沙星含量的一种新方法。

What precautions should be taken to conduct the oxygen flask combustion method

氧瓶燃烧操作的注意事项有哪些？

Shake flask culture was studied and the optimum condition was established.

对摇瓶发酵进行了比较系统的实验，确立了适宜的发酵条件。

The cells were cultured in 24-pore plate and 25cm2 plastic culture flask.

分别在24孔板和25 cm2塑料培养瓶内培养。

The outcome is bad in fibronectm-coated plate or 25 cm2 culture flask.

应用纤维连接蛋白和25cm2细胞培养瓶培养的效果不佳。

The outcome is bad in fibronectin-coated plate or 25 cm2 culture flask.

应用纤维连接蛋白和25 cm2细胞培养瓶培养的效果不佳。

Once the cells reached 80% confluency, they were inoculated in culture flask at the density of 1.0×107 cells/L for proliferative culture.

细胞长到80%融合时，以1.0×107 个/L的密度接种于培养瓶中进行扩增培养。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。