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Iron chloride solution: Add 0.35 g of finely powdered green iron chloride tetrahydrate* and 1.5 mL of dimethyl sulfoxide* to a 5 mL pear shape flask.

氯化亚铁溶液：将0.35g磨细的绿色四水合氯化亚铁*和1.5mL的二甲基亚枫*加到5mL的梨形瓶中。

The optimal shaking flask fermentation conditions were: initial pH 5.5,inoculum 15%,volume charge 135 ml in 250 ml flasks,and nitrogen source 10 ml mixed with 10 g/L yeast extract and 20 g/L tryptone.

摇瓶分批发酵工艺条件的最佳组合是：起始pH5.5，接种量15%，装液量135mL，氮源加入量为10mL含有10g/L酵母膏和20g/L蛋白胨的有机氮源。

The medium composition and some fermentation parameters were studied by cross test and single factor experiments. The optimal conditions are as flows: Tryptone 0.76 %, Bean powder 1.0 %,initial pH 8.0, temperature 28 ℃, inoculation quantity 8 % and loading volume 30 %. Under optimal conditions, the maximum yield of biomass and microbial lipids reached 29.8 g/L and 11.7 g/L, respectively, which was 2.59 times and 2.05 times of those cultured in the original medium, after 9 days fed-batch co-fermentation in shake-flask.

采用单因子和正交实验对培养基组成和发酵条件进行了优化，结果表明，诱变菌在温度28 ℃，pH 8.0，接种量8 %，装液量30 %，蛋白胨0.76 %和豆粕1 %，分批补糖的最优发酵条件下发酵9 d，生物量和菌体油脂积累量分别达29.8 g/L和11.7 g/L，较出发菌提高了2.59倍和2.05倍，同时原料糖利用率达到99.4 %。

ColipET32/IL-11/BL(DE21 recombinant human interleukin-11 in the flask were studied by orthogonal experiment design,including the of culture medium(glucose,tryptone,yeast extract and pH),shaking speed,medium volume and inoculum.

为了探讨发酵条件对大肠杆菌表达人白细胞介素-11融合蛋白的影响，利用正交实验设计，对工程菌的生长条件和人白细胞介素-11融合蛋白表达进行优化。

Coli pET32/IL-11/BL(DE21 that expressed recombinant human interleukin-11 in the flask have been studied by orthogonal experiment design, including the components of culture medium (glucose, tryptone, yeast extract and pH), shaking speed, medium volume and inoculum concentration.

为了探讨发酵条件对大肠杆菌表达人白细胞介素-11融合蛋白的影响，利用正交实验设计，对影响工程菌的生长及重组人白细胞介素11融合蛋白的表达因素如培养基中的葡萄糖浓度、蛋白胨浓度、酵母提取物浓度、无机盐浓度、pH及摇床转速、装液量、接种量等在摇瓶中进行了初步的研究。

The size of round bottom flask, temperature and water addition were the key of volatile oil extraction of C. uncinata, and addition of N-hexane was helpful for the extraction and collection of volatile oil.

圆底烧瓶的大小、温度及加水量是柱果铁线莲挥发油提取的关键，而添加正己烷有利于挥发油的提取与收集。

When feeding with undecylenic acid by flask culture, weight of PHA and PHA content were 2.148 g/L, 51.8% respectively.

锥形瓶馈加十一烯酸培养实验，於培养40小时取样可得最高菌量、最高PHA累积量分别为4.14g/L、2.148g/L，PHA含量可提升至51.8%。

Allow the flask to stand, unstoppered, for about 10 minutes or until all alcohol-wetted surfaces have dried.

直立容量瓶，打开瓶盖约10分钟，使所有的醇润湿表面变干。

The effects of the ratio of carbon source,nitrogen source and concentration of ethanol in the medium on the productivity of polysaccharide-peptide by Coriolus versicolor were studied during the shaking flask liquid fermentation.

通过液体发酵的摇瓶实验，考察碳源、氮源、碳氮比和乙醇浓度对云芝糖肽的产率影响，确定葡萄糖和玉米淀粉为适宜碳源，蛋白胨和黄豆饼粉为适宜氮源，合适的碳氮比为20∶1，适宜的乙醇体积分数为1.5%。

Rinse the inside of the flask with clean wanter before filling for the first time.

第一次添装前请用净水冲洗 2。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。