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In this study, the effects of culture conditions on the growth of recombinant E. coli BL21 (DE3) and the expression of subunit protein of Pasteurella multocida toxin 1 (Tox1) were investigated in both shaking flask and fermentor.

摘要 本研究探讨携带有猪萎缩性鼻炎次单位毒素Tox1基因之重组大肠杆菌大量表现重组蛋白质的最适条件，并对Tox1进行部份纯化。

Under the studies on conditions of liquid fermentation of Poria cocos,the techniques of Erlenmeyer flask additional medium liquid fermentation and additional medium liquid fermentation in fermentor were studied in this paper.

本文在茯苓摇瓶液体发酵条件研究的基础上，进一步探讨了茯苓的摇瓶补料培养液体发酵和发酵罐补料液体发酵的发酵工艺。

4Mm harvested in preculture, is the best. In this form, Rhizopus oryzae R1021 produced most L-lactic acid (76. 62g/L), the pellet size in production medium in flask and fermentor are 1. 9mm and 2. 6mm, respectively, and its L-lactic acid productivity reached or exceeded that of the immobilized cell with polyurethane foam. The produced L-lactic acid and the yield of L-lactic acid based on the total glucose and the productivity are above 74g/L and 74% and 2. 69g/L h, respectively, in 7 batches repetitive fermentation in fermentor, and the size of pellets remain 2. 6mm～3. 4mm.

摇瓶种子培养获得的菌球直径为1.4mm时，这种大小的菌球产酸最高，为76.62g/L；研究表明以直径1.4mm的菌球为种子，在摇瓶发酵和发酵罐发酵中菌球大小分别为1.9mm和2.6mm；这种菌球的产酸水平最高，可以达到甚至超过聚氨酯泡沫载体固定化细胞发酵乳酸的水平；在反复7批次发酵罐发酵过程中，菌球的大小始终保持在2.6mm～3.4mm之间，L-乳酸产量、得率和生产能力分别保持在74g/L、74％和2.69g/L h以上。

Dinger, who was one of the pioneers of quantum mechanics, imagined putting a cat, a flask of Prussic acid, a radioactive atom, a Geiger counter, an electric relay and a hammer in a sealed box.

&薛定谔的猫&是最著名的未证实实验之一。1935年，量子力学的先驱之一，艾尔文·薛定谔提出了一个妙想：把一只猫、一个装有氢氰酸的烧瓶、一枚放射性原子、一个盖格计数仪、一个继电器和一把锤子一起放置到一个密封的盒子中组成一个装置，如果放射性原子衰变，盖格计数仪将探测到放射性，之后向继电器发送信号使其断开，从而释放之前被固定的锤子，锤子会将砸碎烧瓶并释放其中的氢氰酸，盒子里的猫于是被毒死。

In 1935 Erwin Schr dinger, who was one of the pioneers of quantum mechanics, imagined putting a cat, a flask of Prussic acid, a radioactive atom, a Geiger counter, an electric relay and a hammer in a sealed box.

&薛定谔的猫&是最著名的未证实实验之一。1935年，量子力学的先驱之一，艾尔文·薛定谔提出了一个妙想：把一只猫、一个装有氢氰酸的烧瓶、一枚放射性原子、一个盖格计数仪、一个继电器和一把锤子一起放置到一个密封的盒子中组成一个装置，如果放射性原子衰变，盖格计数仪将探测到放射性，之后向继电器发送信号使其断开，从而释放之前被固定的锤子，锤子会将砸碎烧瓶并释放其中的氢氰酸，盒子里的猫于是被毒死。

3 Reweigh, dilute to volume, stopper, then mix by inverting the flask several times.

再称量一次，稀释至体积，盖上塞子，翻转容量瓶数次混合溶液。

Methods: The three lung cancer cell lines-A549(highly invasive human lung adenocarcinoma cell ), 95-D(highly metastatic human lung carcinoma cell), and SPC-A-1(lowly metastatic human lung adenocarcinoma cell) were cultured conventionally in vitro. When the cells covered the bottom of culture flask, we abstracted the protein with lysis solution containing RIPA and PMSF, and obtained RNA with RNeasy Mini Kit.

将三株肺癌细胞：高侵袭的肺腺癌细胞(A549)、高转移的肺癌细胞(95-D)、低转移的肺腺癌细胞(SPC-A-1)做常规细胞培养，待细胞长满瓶底后，用RIPA和PMSF组成的细胞裂解液提取细胞蛋白质，RNeasy Mini Ki提取RNA。

In order to study the effect of carbon sources on the growth of suspension Roselle cell and it's nutrients assimilation, sucrose 、 glucose and soluble starch were used as carbon sources in flask culture of Roselle.

摘 要 采用了蔗糖、葡萄糖、可溶性淀粉3种不同碳源，考察了在摇瓶培养过程中，这3种碳源对维持悬浮培养过程中玫瑰茄细胞的生长及主要基质消耗的影响。

Morgenes cleared a place on the table and then—holding his flask carefully—vaulted up with surprising ease to sit, slippered feet dangling half a cubit above the floor.

Morgenes把桌子清理一块出来，小心地拿着水杯，让人惊讶地轻而易举地在桌子上坐了下来，穿着拖鞋的一脚在地面半腕尺的地方摆动着。

For some reason, if a strag (strag: non-hitch hiker) discovers that a hitch hiker has his towel with him, he will automatically assume that he is also in possession of a toothbrush, face flannel, soap, tin of biscuits, flask, compass, map, ball of string, gnat spray, wet weather gear, space suit etc., etc.

出于某种原因，如果一个旅行者发现了一个带着毛巾的漫游者，他会自动假设这个家伙也会有牙刷、洁面乳、肥皂、一听饼干、瓶子、指南针、地图、线团、杀虫剂、防寒服、宇航服等等等等。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。