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When the inoculative density of MSCs is l×l0~5cells/ml and the microcarrier Cytodex-3 concentration is 10mg/ml, MSCs cultivated in RWV for five days can expand more than nine times but only four times in T-fiask in one generation. Moreover, the culture parameters show that the media pH value in RWV is 7.4-7.6, and the glucose content is lower but the lactic acid content is higher in RWV than in T-flask.

同时对静态和动态培养的细胞进行形态、pH值、渗透压、葡萄糖、乳酸、流式检测及向成骨细胞诱导，发现旋转壁式生物反应器中培养兔骨髓间充质干细胞时，培养基的pH值在7.4-7.6之间，渗透压变化不大，与细胞在培养瓶中培养比较可见葡萄糖含量低而乳酸含量高。

Ethanol yield could reach 20.52 g/L (74.5 %of theoretical yield) under the following conditions: fermentation temperature at 28 ℃, initial pH value as 5.0, 150 mL liquid loadage in 250 mL rotating flask, and rotating speed at 160 r /min, and 0.5 % corn steep liquor and 0.024 % urea. Tran. by YUE Yang

在28 ℃，初始pH值5.0，160 r/min，250 mL三角瓶装液量150 mL，木糖浓度60 g/L，玉米浆0.5 %，含尿素0.024 %的条件下，休哈塔假丝酵母发酵木糖能产乙醇20.52 g/L，得率达到理论值的74.5 %。

The crudeglycerol from biodiesel production could be directly converted to 1,3-PD without any priorpurification. In flask cultures, the 1,3-PD yields of 0.62 and 0.44mol/mol on crude glycerolfrom lipase-and alkali-catalyzed methanolysis of soybean oil were lower than that of0.68mol/mol on pure glycerol. In fed-batch fermentations, the 1,3-PD yields of 0.47 and0.46mol/mol on crude glycerol were comparable to that of 0.49mol/mol on pure glycerol.

生物柴油的副产物甘油不经纯化可以直接用来发酵生产1，3-丙二醇，在摇瓶培养中，由酶法和碱法得到的甘油获得的1，3-丙二醇的转化率分别为0.62mol／mol和0.44mol／mol，而纯甘油得到的转化率为0.68mol／mol；在批式流加发酵中，由酶法与碱法得到的甘油和纯甘油得到的1，3-丙二醇的转化率相差不大，分别为0.47mol／mol、0.46mol／mol和0.49mol／mol，纯甘油得到的生产强度最高，为2.0g／，两种粗甘油得到的生产强度都为1.7g／。

Stock Solution— Dissolve 34 g of monobasic potassium phosphate in about 500 mL of water contained in a 1000-mL volumetric flask.

贮备液：将34g磷酸二氢钾在1000mL容量瓶中溶解于约500mL水中。

Olfactory bulb was abstracted to culture the human fetal OECs, and the monoplast suspension were made and cultured in the uncoated culture flask.

选用流产胚胎标本24份，胎龄4~6月，由西安交通大学第二医院和陕西省妇幼保健院产科提供。

Simple modified differential adherence: monoplast suspension at 8 000/cm2 was incubated in uncoated 25 cm2 flask twice for 12 h +24 h subsequently, the cell suspension was drawn off.

单纯改良差速贴壁：将单细胞混悬液以8 000个/cm2密度种植于无包被的25 cm2培养瓶中。

Determine Nematicidal of Ferment Liquid of Snf 907-76 and Isolation and Purification Nematocide LIU Dan-dan, DUAN Yu-xi, CHEN Li-jie (Shenyang Agriculture University Nematode Laboratory, Shengyang 110161, China) Abstract: Bwauveria bassiana shown strong nematocide abilities and produced nematocide substance by flask ferment.

Snf 907-76发酵液杀线虫活性测定及活性物质分离纯化刘丹丹，段玉玺，陈立杰(沈阳农业大学植物线虫实验室，沈阳 110161)摘要：球孢白僵菌摇瓶发酵后，产生高杀线虫活性物质。

After allowing 1 day for adherence to culture flask, nonadherent cells were removed.

三、脐带血造血前体细胞在间充质干细胞微环境中的增殖动力学研究。

The result of this study indicated that the optimum growth condition of GS-101 on agar medium are at 26℃ in pH 5, and the carbon and nitrogen sources are fructose, and corn steep liquor. Through the experiment of shaking flask, the starch was approved to improve the biomass of the culture. However, without starch the fermented broth oppositively has the activity of inhibiting fibrosarcoma cell (HT-1080) and breast cancer cell (MDA-MB-231) cell.

研究结果显示，GS-101使用洋菜培养基探讨较适生长条件为温度26℃，pH 5，碳源为fructose，氮源为corn steep liquor；从摇瓶试验得知starch虽能增加菌丝乾重，但没添加starch的发酵液具有抑制纤维肉瘤细胞(HT-1080)和乳癌细胞(MDA-MB-231)的活性。

Gerbera plantlets in 125 ml flask received 0, 2.5, 5, 10, 20 ppm Ethrel and 0.01, 0.1, 1 mM 1-aminocyclopropane-1-carboxylic acid to study the effect of ethylene and ethylene biosynthesis through cultured in vitro. The good quality plantlets which had more adventitious roots, no overgown stem, no etiolated plantlets by the addition of 5, 10 ppm Ethrel.

非洲菊(Gerbera jamesonii， Bolus)幼苗於试管内处理以0， 2.5， 10， 20 ppm的益收素及0.01， 0.1， 1 mM的环丙烷氨基羧酸(1-aminocyclopropane-1-carboxylic acid， ACC)以研究乙烯对试管苗之影响及乙烯生合成，结果以5及10 ppm的益收素处理能得到良好品质的苗，为多侧根不徒长，不白化的幼苗。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。