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An primary optimization for medium composition in shake flask showed the best carbon source, nitrogen source , culture volume , incubating quantity and pH after having completed studying the relation between thalli form,zymotic condition and metabolized yields .

通过摇瓶培养，研究发酵条件对菌体形态、产物产量的影响，初步优化了发酵培养基组成，确定了最佳碳源、氮源、装液量、接种量、pH等。

Compared with alcoholmeter method, its measurement results were more accurate. The alcohol content measurement results by chemical oxidation and by international density flask method were the same.

在这两个方法中，密度瓶法作为经典的分析方法，结果准确，但是，20℃的恒温条件不易控制，这就势必造成一定的分析误差；而酒精计法虽然具有操作简便快速，但由于酒精计精度较差和人为因素，导致误差较大。

The results showed that the optimal carbon sourceand nitrogen source were amidulin,CaCO_3 and yeast.The optimization pH 7.0～8.0,cultural temperature:30—32℃,and germ Q6 was cultured for 48h inbroad-openning flask with 20% volume medium.

结果表明，在起始pH值7.0～8.0，接种量为5％以上，培养基装量为广口三角瓶体积的20％以下，培养温度30—32℃，振荡培养48h时Q6发酵产物的抑菌活性最强。

KEY WORDS］Apocynum venetum; antibiotic property; shake flask method; bacteriostasis rate

关键词］罗布麻纤维；抗菌性；烧瓶振荡法；抑菌率

The primary cultured Müller cells adhered to the wall of flask and grew fast, with large applanate cell bodies.

原代培养的Müller细胞贴壁生长，胞体较大、扁平，第3代细胞生长减慢。

A small flask-shaped fruiting body in ascomycetous fungi that contains the ascospores.

担子菌的一个小目，肉质，球状子实体；包含马勃菌和地星。

Aseptically remove a sufficient quantity of solids from the appropriate amount of containers (see Table 2), mix to obtain a composite, equivalent to about 6 g of solids, and transfer to a sterile 500-mL conical flask.

以无菌操作从适当数量的容器中（见表2）取出足够数量的固体，混匀以获得等同于6g固体的混合物，转移至一个无菌的500mL锥形烧瓶。

Results The endocrine cells found in the DT were round or oval, spindle, shuttle, pyramid, flask and bacilliform in shape.

结果蓝尾石龙子消化道中的内分泌细胞形态多样，呈圆形、椭圆形、纺锤形、梭形、锥形、烧瓶形和杆状。

In this paper, an efficient and easy biological screening method based on the sensitivity to lovastatin of Neurospora crassa was built to deal with the mutant strain rudely by UV mutation. A high-level strain was screened in the shaking flask culture incorporating with TLC and HPLC. A comparison between this two methods also was originally discussed while identifying dyeing using bromophenol blue as the better treatment of TLC, and offering a kind of simple, practical, fast and economical determination qualitatively and quantitatively.

本文有鉴于此，通过紫外诱变处理，并基于粗糙脉孢酶对洛伐他汀的生物敏感性，对诱变菌株进行了初筛；同时，采用摇瓶培养，结合TLC和HPLC的分析方法，复筛出一株高产菌株；对复筛过程两种分析方法分别进行了初步探讨，比较其优劣，确定TLC法经溴酚兰显色剂显色处理为较佳方式；从而，建立了一套简单、实用、快速、经济且有效的洛伐他汀生产菌诱变筛选方法。

At present study we show that aseptic flask grown plantlet of Cyrtopodium paranaese and Mormodes Jumbo Odin × Catasetum expensum can form "rootball" spontaneously at root tips and root middle parts under no influence of hormones, and then regenerate into plantlets.

吾人研究发现曲足兰与妖精兰×瓢唇兰在组织培养下，不需荷尔蒙就自然会在根的尖端或是中间形成类似原球体构造的组织，再发育成为完整的植株，此种现象在曲足兰园艺栽培上已有记载并称之为根球。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。