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The fermentation culture medium and shaking flask fermentation conditions of glutathione have been researched by using orthogonal experiments with Saccharomyces cerevisiae.

通过正交试验，研究了啤酒酵母摇瓶发酵生产谷胱甘肽的培养基的组成以及发酵培养条件。

The effect of nitrogen source, initial pH, quantity of incubated in flask, culture temperature and time on producing chitinase of Pseudoalteromonas H7 were studied systematically in this paper.

系统地研究了氮源、起始pH、培养基装量、培养温度和时间等因素对交替假单胞菌H7产甲壳素酶的影响。

After repeated short time digestion of pancreatic tissues with collagenase Ⅴ, cultured cells were transferred to a new culture flask.

用胶原酶反复短时间消化胰腺组织，在细胞接种后及时转板的方法可获得生长良好的高纯度胰岛原代单层细胞，适合用于实验研究。

Therefore, the biomass concentration during flask culture can be predicted by this neural network model if initial glucose concentration and initial biomass concentration were provided.

所以，由培养液的初始葡萄浓度和初始生物量浓度通过网络软测量模型就可对摇瓶中微生物的生物量浓度进行估算。

High level of Kal was obtained in BMMY medium (pH 7.0) after 96 hours induction of 29?C and 2% methanol, with the highest yield of 14 mg/L in shake flask culture.

阳性表达菌株在BMMY培养基(pH 7.0)中29℃培养，经2％甲醇诱导表达96 h，摇瓶表达量可达14 mg/L。

100ML culture flask, nitinol wire, cardiac pacemaker and connecting lead were used to make electric field.

使用一次性100mL培养瓶、镍钛合金金属丝、心脏起搏器和连接导线设计制作电场。

Under the optimum concentration of 1.5% glycerol in fermentation medium and feeding of 1% glycerol as precursor after 60 h of incubation, the titer of 2,668 μg/ml was obtained in shake flask culture, an increase of 7.4 times compared with the original strain.

以XC-5-48菌株为试验菌株，当培养基中甘油初始浓度为1.5%，并且在发酵60h时补加1.0%的甘油，摇瓶发酵的效价可达2668μg/ml，较出发菌株(318μg/ml)提高了7.4倍。

Results The open cycling air pressure control culture system worked effectively, the pressure inside the culture flask can be controlled from 0 to 100 mm Hg.

结果成功组装开放式压力控制培养系统，通过调节进出气流速度，可将培养瓶内压力控制在0～100 mm Hg（1 mm Hg=0.133kPa）之间。

The hyaluronic acid production by batch and fed batch culture s in flask and fermentor was studied, and the primary mechanism was discussed.

在耗糖量相同的情况下，分批发酵比多次加料或流加发酵具有更高的HA产量和转化率；分批发酵初糖7％，发酵24h 左右，产HA3。

Once the microsphere was fractured and in contact with the surface of culture flask, cells spread and attached onto the plastic substratum and proliferated.

当球体破裂而接触培养皿表面时，细胞会在塑胶基质上有吸附与伸展的现象并且开始增生。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。