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The effects of cultivation conditions such as temperature, intial pH, and broth volume on the bioacitivities of the biotransformation products of EGB by Hericium erinaceus in shaking flask were further investigated.

在前期优化培养基的基础上，本实验比较了摇瓶的温度、pH、装液量和发酵罐培养的通气量、转速和培养时间等不同培养条件对猴头菌转化EGB生物活性的影响。

In this thesis, the polymerization of triblock copolymer of styrene-butadiene-methyl methacrylate was carried out in glass flask ,with n-BuLi as initiator, 1,1-diphenylethylene as capping agent and Lithium 2(2-methoxyethoxy) ethoxide as additive.

本论文以n-BuLi为引发剂、1，1-二苯基乙烯为戴帽剂、2-(2-甲氧基乙氧基)乙氧基锂为添加剂，对玻璃聚合瓶中非极性-极性三嵌段共聚物聚苯乙烯—聚丁二烯—聚甲基丙烯酸甲酯的合成进行了研究。

The oxygen-flask cormbustion method is used to determine the total phosphorus in cotton seed using lanthanum nitrate as titrant and haematoxylin as indicator.

氧瓶燃烧法以硝酸镧标准溶液做滴定剂，苏木精为指示剂，测定棉籽中的总磷。

Amplified cells were cultured in PGC culture medium and non-adherence culture flask till idiosome formed, adhered and differentiated.

体外分化采用无黏附特性的培养瓶和除去白血病抑制因子的原始生殖细胞培养液，以脱饲养层法悬浮培养扩增的细胞，直到拟胚体形成及贴壁分化。

At the fifth passage, two cell strains were made into cell suspension at l×10^9L^(-1). 0.5 mL was subcutaneously incubated at the inguina of each rat for 8 weeks. Amplified cells were cultured in PGC culture medium and non-adherence culture flask till idiosome formed, adhered and differeotiated.

体内实验取连续传5代的2个细胞株，分别制备浓度为l×10^9L^(-1)的细胞悬液，于每只小鼠两侧腹股沟处皮下各接种0.5 mL，培养8周体外分化采用无私附特性的培养瓶和除去白血病抑制因子的原始生殖细胞培养液，以脱饲养层法悬浮培养扩增的细胞，直到拟胚体形成及贴壁分化。

When the inoculative density of MSCs is l×l0~5cells/ml and the microcarrier Cytodex-3 concentration is 10mg/ml, MSCs cultivated in RWV for five days can expand more than nine times but only four times in T-fiask in one generation. Moreover, the culture parameters show that the media pH value in RWV is 7.4-7.6, and the glucose content is lower but the lactic acid content is higher in RWV than in T-flask.

同时对静态和动态培养的细胞进行形态、pH值、渗透压、葡萄糖、乳酸、流式检测及向成骨细胞诱导，发现旋转壁式生物反应器中培养兔骨髓间充质干细胞时，培养基的pH值在7.4-7.6之间，渗透压变化不大，与细胞在培养瓶中培养比较可见葡萄糖含量低而乳酸含量高。

Ethanol yield could reach 20.52 g/L (74.5 %of theoretical yield) under the following conditions: fermentation temperature at 28 ℃, initial pH value as 5.0, 150 mL liquid loadage in 250 mL rotating flask, and rotating speed at 160 r /min, and 0.5 % corn steep liquor and 0.024 % urea. Tran. by YUE Yang

在28 ℃，初始pH值5.0，160 r/min，250 mL三角瓶装液量150 mL，木糖浓度60 g/L，玉米浆0.5 %，含尿素0.024 %的条件下，休哈塔假丝酵母发酵木糖能产乙醇20.52 g/L，得率达到理论值的74.5 %。

The crudeglycerol from biodiesel production could be directly converted to 1,3-PD without any priorpurification. In flask cultures, the 1,3-PD yields of 0.62 and 0.44mol/mol on crude glycerolfrom lipase-and alkali-catalyzed methanolysis of soybean oil were lower than that of0.68mol/mol on pure glycerol. In fed-batch fermentations, the 1,3-PD yields of 0.47 and0.46mol/mol on crude glycerol were comparable to that of 0.49mol/mol on pure glycerol.

生物柴油的副产物甘油不经纯化可以直接用来发酵生产1，3-丙二醇，在摇瓶培养中，由酶法和碱法得到的甘油获得的1，3-丙二醇的转化率分别为0.62mol／mol和0.44mol／mol，而纯甘油得到的转化率为0.68mol／mol；在批式流加发酵中，由酶法与碱法得到的甘油和纯甘油得到的1，3-丙二醇的转化率相差不大，分别为0.47mol／mol、0.46mol／mol和0.49mol／mol，纯甘油得到的生产强度最高，为2.0g／，两种粗甘油得到的生产强度都为1.7g／。

Stock Solution— Dissolve 34 g of monobasic potassium phosphate in about 500 mL of water contained in a 1000-mL volumetric flask.

贮备液：将34g磷酸二氢钾在1000mL容量瓶中溶解于约500mL水中。

Olfactory bulb was abstracted to culture the human fetal OECs, and the monoplast suspension were made and cultured in the uncoated culture flask.

选用流产胚胎标本24份，胎龄4~6月，由西安交通大学第二医院和陕西省妇幼保健院产科提供。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。