查询词典 flanking

Analyzing T-DNA flanking sequences isolated by TAIL-PCR revealed that the T-DNA nick position in the tagged rice plants are more complex than in tansgenic dicot species as tobacco and Arabidopsis.

分析TAIL-PCR分离的T-DNA侧翼序列，发现水稻转化体中T-DNA右边界的剪切位点比转基因拟南芥和烟草等双子叶植物中T-DNA右边界的剪切位点要复杂得多。

Two SP1-like elements and half of binding motifs of RAR are found in the 119 bp region from -nt. 325 to -nt. 207 upstream of the first ATG. An AP1/cAMP like reaction element is found in the 113 bp region from -nt. 206 to -nt. 94 upstream of the first ATG of the CK13 gene 5' flanking region. We didn't know the reaction element CTGAATCA between -nt.

仔细分析CK13基因5'旁侧-nt.325~-nt.207之间119bp片段中，发现有两个类似的SP1反应元件及一个与半个维甲酸受体结合的模块，而CK13基因5'旁侧-nt.206~-nt.94之间113bp片段中-nt.199~-nt.192间的碱基CTGAATCA类似AP1/cAMP的反应元件。

In addition, these data indicate that the nucleotide sequences flanking the histamine biosynthetic genes in Staph.

此外，这些数据表明，核苷酸序列的侧翼，在金黄色葡萄球菌组胺生物合成的基因。

In molecular phylogebetic tree constructed, we made the Ka / Ks value pairwise comparison of HMGbox flanking sequences both intra- and inter- Hominoid and the Old World m onkey .

通过构建系统发育树，比较旧大陆猴和人猿超科两个类群内和类群间HMG盒侧翼序列K a/Ks的比率。

Anti-histidine-tag antibody is usually used for identification of expression of the recombinant protein expressed using pRSET-A. A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence.

质粒pRSET-A是一个常用的高效原核表达载体，编码一N端含组氨酸标签（6×His）的34aa前导肽序列，以方便利用抗组氨酸标签抗体鉴定或纯化所表达的重组蛋白。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

The production of human alpha lactalbumin was 0.64 g/L approximately. The results indicate that the bovine mammary epithelial cell line have the ability of expressing exogenous gene, the 5′ flanking sequence of bovine αS1 casein gene has the effect of regulating gene expression specifically and the vector αS1-LA-psv which was constructed can coexpress alpha lactalbumin and β-galactosidase.

实验结果表明，建立的奶牛乳腺上皮细胞系具有外源基因表达活性，得到的牛αS1-酪蛋白5′调控序列能作为启动子指导外源基因的表达，构建的真核表达载体能在体外培养的牛乳腺上皮细胞中同时表达人α-乳白蛋白和β-半乳糖苷酶。

PCR-RFLP in FSHR gene 5'–flanking region with exon 1 digested by restriction endonuclease TaqⅠdemonstrated that the frequencies of allele A (0.5500) and genotype AA (0.5000) in twinning cows were higher than that of allele A (0.3500) and genotype AA (0.3000) in monovular cows for Qinchuan cattle, while The frequencies of allele B (0.5937) and genotype BB(0.6875) in twinning cows were higher than that of allele B (0.5416) and genotype BB (0.4167) in monovular cows for Holstein cattle. Key words: Bovine; Twinning; FSHR gene; PCR-RFLP

FSHR基因5'端（包括侧翼序列和第一外显子）的限制性内切酶TaqⅠ的PCR-RFLP标记研究发现，在秦川牛中双胎母牛的A基因频率（0.5500）和AA基因型频率（0.5000）分别高于单胎母牛的A基因频率（0.3500）和AA基因型频率（0.3000）；在黑白花奶牛中双胎母牛的B基因频率（0.5937）和BB基因型频率（0.6875）均高于单胎母牛的B基因频率（0.5416）和BB基因型频率（0.4167），也就是FSHR基因的5'端的PCR-RFLP标记在一个品种内单胎母牛和双胎母牛之间有一定趋势，但两个品种的趋势不同。

It was the first time that this phenomenon was found. Furthermore, fragments flanking the conservative region were found different in molecular weight in genome of different navel orange cultivars.

此外，对不同脐橙基因组中LCYB基因保守序列两端进行Tail-PCR扩增时，发现其两端扩增片段的分子量普遍存在差异。

Alkaline nuclease gene and its flanking region of Bombyx mori nucleopolyhedrovirus strain Suzhou was cloned, sequenced, and analyzed.

基于为杆状病毒分类提供新的依据，克隆了家蚕核型多角体病毒苏州株碱性核酸酶基因及其侧翼区，并进行序列和进化分析。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了