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Methods:Human myeloma cells(KM3) and bone marrow stromal cells were fixed by paraformaldehyde.

目的：探讨白细胞介素6（IL-6）在多发性骨髓瘤中过度表达的机制。

The samples were fixed with paraformaldehyde and embedded in paraffin.

应用免疫组织化学技术检测鼻粘膜组织中水通道蛋白1、2、3、4、5的分布情况。

Result The spindles of oocytes were abnormal at 25℃ for 20 min, and disappeared at 25℃ for 30 min or at 4℃ for 10 min. 5, 10, 20 and 40 μg/ml Cytochalasin B for 2 h had no effects on the spindles of the oocytes. The spindles of oocytes were abnormal in 20 μmol/L colchicines for 1 h, and disappeared in 40 μmol/L colchicines for 1 h. The structure of spindles were perfect for 1-12 h when the oocytes were fixed in 4% paraformaldehyde.

结果］卵母细胞在25℃、20 min时纺锤体异常，25℃、30 min或4℃、10 min时纺锤体缺失；5、10、20和40μg/ml细胞松弛素B作用2 h未对纺锤体产生影响；卵母细胞在20μmol/L秋水仙素中作用1 h纺锤体异常，40μmol/L时作用1 h纺锤体缺失；在4%多聚甲醛中4℃条件下，卵母细胞纺锤体结构以固定时间为1~12 h效果较好。

Methods After fixed by paraformaldehyde, vascular endothelial cells from pigs were co-cultured in vitro with natural killer cells from Chinese monkeys at different ratios. The change of the cytotoxicity of natural killer cells occurring after this contact and the content of IFN-γ and TNF-α in the supernatants were detected.

将中国猕猴的NK细胞与多聚甲醛固定后的猪血管内皮细胞按不同比例进行体外混合培养，检测NK细胞在以不同比例接触靶细胞后的杀伤活性变化以及培养液上清液中的IFN-γ及TNF-α含量。

Acquisition of high-quality chromosome suspension: The chromosome suspension released from homogenization suited for flow karyotypic analysis and sorting after fixed in 2% paraformaldehyde for 20 minutes and purified through 10% and 30% sucrose concentration gradient.

高质量染色体悬液制备结果：同步化的根尖细胞经2%多聚甲醛固定20 min，机械匀浆释放染色体并结合10%及30%蔗糖浓度梯度纯化释放的染色体悬液。

The optimum dyeing conditions were shown: viral samples fixed with 0.1% paraformaldehyde for 30 min at 4℃, stained with SYBR Green I 1 × 10^(-4 commercial concentration for 10 min at 80℃.

杆状病毒最佳染色条件：质量分数为0.1%的多聚甲醛固定病毒30min后，加入一定量的SYBR Green I在80℃下孵育10min。

Suddenly she looked out the window and saw Pete the Parakeet in the tree out side the window and his eyes were fixed on her.

她望着窗外，突然看见鹦鹉在树上的特列方和他的眼睛望着窗外她。

Because every are folded,flow board standard limit and elliptical long gross ril load close or parallel, and with a center of the each canal hole that the triangle forms cloth to be in charge of connects line parallel; another edge also with the Guan Kongzhong of another direction the heart connects line parallel, the fixed position that consequently its provide opening is underlined and make easy implementation.

由于每块折流板的基准边和椭圆之长轴重合或平行，并和正三角形排列布管的各管孔的一条中心连线平行；另一条边也和另一方向的管孔中心连线平行，因而其管孔的定位划线和制造轻易实现。

It is formed from controllable slide block mounted in guideway, length-fixed drive rod capable of driving slide block and making it move in guideway, controllable telescopic shaft and mobile and turnable working table consisting of moving platform for mounting cutting tool and working table for mounting workpiece, and utilizes the drive rod, controllable telescopic shaft, moving platform, slide block and guideway to form the invented redundant drive plane parallel closed link mechanism with several functions of cutting, grinding, load-shifting and assembling, etc.

由安装在导轨中的可控滑块、驱动滑块在导轨中运动的定长驱动杆、可控的伸缩轴及安装切削刀具的动平台、安装工件的工作台构成的可移动和转动的工作台组成。并由驱动杆、可控伸缩轴、动平台、滑块、导轨构成冗余驱动平面并联闭链结构。该装置既具有切削、磨削、移载、装配等多种功能，又具有简单结构、大作业空间和刀具实现姿态能力，以及优先的加工方向等优点的并联机床新结构。

The quadratic patches is fixed,and its parametric representation method is gained.The cubic patches reduce to three local factors through reducing Bernstein coefficients to control shape of curve,two of them are used to join segments into G2- continuity approximating curves for given sequence data in space,and the other is used to adjust shape of segment.

先固定二次曲面片，并得到其参数形式，然后约简三次曲面片所对应的Bernstein系数，使之为带有三个形状调整的形状因子，其中两个分别代表曲线段端点处的曲率，另外一个作为形状的调整。

Objective To investigate the relationship between ovum fertilization rate and sperm morphology, acrosome reaction in IVF and to explore the influence of parameters of sperm on fertilization rate.

目的 研究体外受精(in vitro fertilization，IVF)中精子形态、顶体反应与卵子受精率之间的关系，分析精子各参数在IVF受精率方面的参考意义。

A: I hear Mr. White wants to run for the Presidency!

我听说怀特先生要参加总统竞选！