查询词典 fish in troubled water

Forepaw of fish, birds and chickens have become a pair of wings, the person becomes a hand and arm, before other animals is sufficient, after the fish into a bird claw on the small claws, and Legs and feet, neck giraffe is the most developed and least developed is the fish,.

鱼的前爪，对于鸟类和鸡就变成了一对翅膀，对于人就变成了手和胳膊，其他动物就是前足，鱼的后爪就变成了鸟的小爪子，和人的腿和足，脖子最发达的就是长颈鹿，最不发达的就是鱼，。

The invention discloses a method to analyze fish genetic information with mitochondria DNA D-loop controlling zone, which comprises the following steps:(1) extracting genom DNA and mitochondria DNA of fish;(2) designing simple primer in fish mitochondria DNA D-loop controlling zone; possessing twenty one basic groups CAC CCY TRR CTC CCA AAG CYA in upstream primer MitD1-F; possessing twenty three basic groups GGT GCG GRK ACT TGC ATG TRT AA in downstream primer MitD1-R;(3) proceeding PCR reaction under the function of primer;(4) choosing agarose jel electrophoresis;(5) checking sequence for augment mtDNA D-loop gene fragment.

本发明公开了一种利用线粒体DNA D-loop控制区进行鱼类遗传信息分析的方法。它包括如下步骤：(1)分别提取鱼类基因组DNA和线粒体DNA；(2)在鱼类线粒体DNA D-loop控制区设计简并引物：其上游引物MitD1-F有21个碱基：CAC CCY TRR CTC CCA AAG CYA；下游引物MitD1-R有23个碱基：GGT GCG GRK ACT TGC ATG TRT AA；(3)在引物的作用下进行PCR反应；(4)琼脂糖凝胶电泳；(5)对扩增的mtDNA D-loop基因片断进行测序。

Evening, has had the dinner, we trade You Yi to go to the gizzard shad immediately to cure the hot spring, in the warm artificial pond has many "cherishes one's relatives the fish", I sit on the chair, puts in the pond the foot, calmly waited for 15 seconds, the small fish to flush to me, nipped my shameless, two, many strip, the small fish pecked me, might itch really.

问题补充：晚上，吃过晚饭，我们马上换好游衣去泡鱼疗温泉，温泉池里有许多"亲亲鱼"，我坐在椅子上，把脚伸进池里，静静地等了15秒，小鱼就向我冲来，咬我的死皮，一条、两条、很多条，小鱼啄我时，可真痒啊。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

My lunch eats in the unit, only selects the element eating, sometimes will eat the fish, but cannot be the oil fish and chips, also will not bump the hairtail and so on cholesterol high fish.

我的午饭在单位吃，只挑素的吃，有时会吃鱼，但不能是油炸鱼，也不碰带鱼之类胆固醇高的鱼。

Does not have some of the fish scales high cholesterol, such as sweet fish, eel, Loach, rice field eel, cod, but not all the fish without scales have high cholesterol, such as hairtail, sharks, with a scale of the grass carp, Changyu , Perch, and other similar.

的确有一些没有鳞的鱼胆固醇较高，如银鱼、河鳗、泥鳅、黄鳝、鳕鱼，但不是所有无鳞的鱼胆固醇都高，如带鱼、鲨鱼，与有鳞的草鱼、鲳鱼、鲈鱼等相似。

They have tanks of live fish swimming around (some, sadly, upside-down or sideways). They also have dead fish sitting in ice. They also have half-cut fish, with blood dripping out of them.

超市里有游着活鱼（有些，很可悲地，已经或快死了）的大鱼缸，也有躺在冰上的死鱼，还有宰了一半的鱼，血淋淋的。

They include lobster; prawn; abalone; pearl-shell; grouper; jelly-fish; sea-cucumber and fifteen types of oysters. There are 580 kinds of fish, 527 kinds of shell fish and 32 kinds of prawn .

这里的海产资源十分丰富，盛产龙虾、对虾、鲍鱼、珍珠贝、石斑鱼、海蜇、海参和各种各样的蚝；计有鱼类580种、贝类527种、虾类32种。

Lantern fish When under attack, Mingkang fish will be used strictly as the head of the tentacles all the fish from the monster walk around with.

当受到攻击时，明康鱼会利用头顶那灯笼般的触须把所有的鱼儿从怪物身边引走。

Powerful information system support is needed at every stage, from fish farming through inspection and live fish center operation through to the supply of fish to restaurants; the systems involved include traceability systems, purchasing, sales and inventory systems, etc.

吴智谋说，RFID是工具，最重要的是资讯平台系统的建立，从养殖端、检验端、活鱼中心畜养，乃至於餐厅的供货情况等，都要有强大的系统作后援。

He paid $5,000 for this cupcake.

他我这个纸杯蛋糕花了5000美元。

I mean, if you want to, that is

我的意思是，如果你们想吃的话。