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In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Said you're going with your boys today and "Don't worry cause I'll be home late" But I know you're creepin' out there, cheatin', You've been freakin' babe So let me tell you how it's going to be And you can run game but not on me boy So who the hell are you trying to kid You know this is what I did I called your pager and your two-way boy but no response Start looking and I found you in some other women's arms Don't try to front boy cause I saw you there with my own eyes Just get your stuff boy cause I'm tired of hearing all your lies I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Baby boy I know you'll never find No stuff as good as mine She can't work you like I did You can look but you know I got that good shit I know that you're hating it But you better stay with the one you're with You made your bed and now you have to sleep in it baby I called your pager and your two-way boy but no response Start looking and found you in some other women's arms Don't try to front boy cause I saw you there with my own eyes Just get your stuff boy cause I'm tired of hearing all your lies Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door I ain't gonna take this no more I ain't gonna take this no more Bounce baby out the door Bounce baby out the door Get up and move Get up and move Don't make me act a fool Don't make me act a fool Just bounce baby out the door Wait a minute Sarah, give me a chance to explain I was just talking to shorty, I don't even know her name I ain't looking for no one to come and take your place Ain't no one out there baby that can fill your space No need to find a nickel got a dime at home Plus I love every night the freaky way we bone I ain't trying to get gone, ain't trying to roll I'd rather stay and be a part of your green eyed soul Just bounce baby out the door Bounce baby out the door Just bounce I ain't gonna take this no more Just bounce Bounce baby out the door Just bounce Get up and move Just bounce Don't make me act a fool Just bounce

我ain't去向前发生作用这个不更多弹起当作婴儿般对待出去门到达向上和移动做不制造我行动a开玩笑仅仅弹起当作婴儿般对待出去门弹起当作婴儿般对待出去门我ain't去向前发生作用这个不更多弹起当作婴儿般对待出去门到达向上和移动做不制造我行动a开玩笑假定你是去用你的男孩今天和"做不担心原因我希望在家迟"但是我知道你是creepin'出去那里cheatin'你已经是freakin 婴儿那样让我说出你，它怎样正将是和你能变得勇敢了但是不是在我上那样地狱对是你试一试的男孩哄骗你的知道这个是我所做的我呼吁你的寻呼和你的双向的男孩但是没有看和我反应出发发现你在情妇手臂不努力尝试的一些中我有我的自己眼睛的仅仅把厌烦你的我被的材料男孩原因带到那里听你所有的谎言处理前面的男孩原因你我ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门到达沿着和移动做不制造我行动a开玩笑仅仅弹起当作婴儿般对待从里面门仅仅弹起弹起当作婴儿般对待从里面门我ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门到达沿着和移动做不制造我行动a开玩笑小男孩我知道你希望从不裁决不材料实际已经开采矿她不能工作你相似我是做你罐看但是你知道我得到那个好排粪我知道那个你是讨厌它但是你更好些留下用一你是用你制做你的床和现在你富人向睡觉在中它当作婴儿般对待我打电话你的寻呼和你的双向地男孩但是没有反应开始看和发现你在中一些情妇's手臂做不请尝试向前男孩原因我拉来拉去你那里用我自己的眼睛仅仅到达你的材料男孩原因我被使厌烦的听见你所有的谎言弹起当作婴儿般对待从里面门我 ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门到达沿着和移动做不制造我行动a开玩笑仅仅弹起当作婴儿般对待从里面门我ain't去向发生作用这个没有更多我ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门弹起当作婴儿般对待从里面门到达沿着和移动到达沿着和移动做不制造我行动a开玩笑做不制造我行动a开玩笑仅仅弹起当作婴儿般对待从里面门仅仅弹起等候a分钟萨拉让步我a机会向说明我是仅仅说话向矮子我做不平稳知道她名字我ain't寻找没有任何人向来和发生作用你的地方Ain't没有任何人从里面那里当作婴儿般对待那个罐充满你的是一部分你的绿色是从从门公正弹起里面门公正弹起弹起小里面盯着精神公正弹起小我ain't去把这没有更公正弹起弹起小带到从门里面仅仅弹起组织和移动公正弹起不使我装出一傻瓜公正弹起的样子

The quality of interior environment in nursing home residents will become one of the attention-getting subjects due to the gradual increase of aging populations This paper directs at the research of nursing home's interior environment factors in Tainan We use the standard of international practice to analyze the intrinsical data of environmental monitoring and establish the characteristics which from environmental physical reaction of nursing home The research items are air environments (PM10 CO CO2 formaldehyde TVOC) temperate environments and light environment (illumination average brightness daylight factor) etc There are the inductive conclusions of this research: 1 In basic medicine the clinical diagnosis means the theory and technique about physical examination with patients The definition of clinical diagnosis in architecture should be the diagnosis of building preserve and improve the feasible way to extend building's service life and assure the residents of the healthy quality of interior environment The object of clinical diagnosis in architecture is not only to ensure the use of structure and environment quality but also keep the structure and environment quality in sustainable; therefore creating an efficient system of preserving plan is needed 2 It is an accurate and technical circumstance which about improving remedying and reconstructing the problems of structure space This circumstance includes finding questions ensuring causation ascertaining ultimate strength evaluating environment quality estimating the necessary of restoration and implementing on a suitable way Furthermore when reconstructing and ameliorating the appearance of structure space it should be respected and the memories of it should be preserved 3 In clinical diagnosis of environment quality of space the question of physical environment and the deficiencies of structure must be separated The structure space of construction and the high quality of space should be inspected strictly then observed the crucial reason that can find out any invisible and hidden causes The diagnosis of construction is to observe the reason of destroyed structure The environmental diagnosis of indoor and outdoor is to find out the crucial reason that influences health 4 In order to decrease the iterant problems the construction and environment of space should be diagnosed However the destruction by natural strength and artificial using by human are important causes that can affect the service life of construction Searching the cause of disease just like curing the patient which includes inspection diagnosis cure and prevention We tested the thermal conductivity of siding material in this research and then found out the roof east vertical face and west vertical face have most radiant heat especially the construction of RC The characteristics of opaquely outer casing have relations with the thermal conductivity of wall to wiz the ability of heat insulation Therefore increasing the efficiency of heat insulation and decreasing the endoergic factor of insolation are very important to temperate environments

而随著国内老年人人口逐渐增加，逐渐迈向高龄化之同时，对於高龄者使用空间之室内环境品质相关议题之探讨，亦成为未来我们需加以关注之议题之一。主要为针对台南某安养中心室内环境因子进行检测调查工作，利用现场室内环境测定以取得有效的实测数据；再藉由汇整相关国际评估基准，并进行实测数据比对分析，建立老人安养中心对物理环境反应之特性。并以前期相关研究文献回顾与老人安养中心实测案例现场的实验，来了解不同空间物理环境特性之影响，并将结果回馈实际建筑及室内设计之用。调查工作项目包含：空气环境之PM10、CO、CO2、甲醛、TVOC；温热环境之室内温度、相对湿度、人体PMV、PPD；光环境之照度、均齐度、昼光率等因子调查。本研究结果可归纳以下几点结论： 1 所谓临床诊断医学乃相对於基础医学而言，是指实际与病人接触的医疗及护理行为中牵涉到的理论和技术；而建筑的临床诊断的定义应为建筑本体与室内外诊断、维护及改善所有可行的方式来延长建筑物的寿命，并确保人与室内环境空间品质的健康。建筑临床诊断目的为确保结构体与环境品质能继续使用，而能维持所需要的效能及承受能力。一个有效的维护计画体系是必须建立的 2 在建筑空间之问题改善、治疗与修复执行中，是一个精确与技术性的事项，其中包含找出问题、确定原因、评估结构应力强度、评估环境品质、评估修复与改善必要性、选择及执行一个适当的过程。其改善与修复也必须尊重建筑空间原有色彩与形貌，并保留原有记忆及文化。 3 在临床诊断中空间环境品质中，物理性环境问题与结构构件缺陷必须分别判断，诊断建筑空间之结构与空间舒适品质必须详细观察其症结性问题，才能发现任何隐藏及有潜伏性缺陷的原因。建筑本体诊断是观察出建筑本体之破坏原因，而是内外空间环境诊断目的在於的找出影响健康因素之症结性问题。 4 建筑本体与空间环境之预防评估目的为降低与抑制问题的再发生。然而，自然力的破坏与人为使用因素皆是影响建筑生命期之主要因素，找出病源问题就如同医生对病人的处理，包含了侦查、诊断、治疗以及预防。 5 本研究以外墙材料热传导率测试结果发现，屋面与东西向立面是承受辐射热最多的地方，尤以 RC 的建筑更甚，不透明部分的外壳能特性主要与壁体的热传透率即隔热能力有关，但是承受来自日射的吸热影响，因此增加隔热性能与降低日射吸热因子是对温热环境是非常重要的。

Because I wanted to find out what you guys found out before I find out what he found out.

因为我想知道在他发现什么之前你们这帮人能发现什么

"But we tried to find the patterns that other researchers had found, and we still couldn't find any predictable pattern," Galvin said.

Galvin 说，"我们已经试着探索其他研究者报道的模式，但我们未能发现任何确定的试验模式。"

I have a vehicle license Wuxi polo, bought two years, to buy time to entrust 4s outside the shop on the card, was also committed to a vehicle from them to solve the problem, the first two months of the time I went to the first annual inspection, and 4s to find stores, they say that nothing we can do, and later became aware of the understanding that they were also looking for the licensing of local cattle, and now that person can not find the time they also搞不定, and later found another cattle probably have to offer 1000, I did not spot anger Oh hematemesis.

我有辆无锡牌照的polo，买了两年了，买的时候委托4s店上的外牌，当时也承诺了验车问题由他们来解决，前两个月我到时间第一次年检了，找到4s店，他们竟然说没办法，后来了解了才知道原来他们当时上牌找的也是当地的黄牛，现在那个人找不到了所以他们一时也搞不定，后来大概找了另一个黄牛开价竟然要1000，我气得没当场吐血哦。

If i die tomorrow I'd be allright Dream Theater Because i believe That after we're gone The spirit carries on I used to be frightened of dying I used to think death was the end But that was before I'm not scared anymore I know that my soul will transcend I may never find all the answers I may never understand why I may never prove What i know to be true But i know that i still have to try If i die tomorrow I'd be allright Because i believe That after we're gone The spirit carries on Victoria:"move on, be brave Because i am no longer here But please never let Your memory of me disappear" Nicholas: Safe in the light that surrounds me Free of the fear and the pain My questioning mind Has helped me to find The meaning in my life again Victoria's real I finally feel At peace with the girl in my dreams And now that i'm here It's perfectly clear I found out what all of this means If i die tomorrow I'd be allright Because i believe That after we're gone The spirit carries on Hypnotherapist: you are once again surrounded by a brilliant white light.

如果我死在明天我就是所有权梦剧场因为我相信之后，我们正在经历精神继承从前我害怕死亡从前我认为死亡是完不过，那是以前我不害怕了我知道我的灵魂将超越我可能永远找不到所有的答案我可能永远不会明白为什么我可从来没有证明我所知道的是事实但我知道我仍然要尝试如果我死在明天我就是所有权因为我相信之后，我们正在经历精神继承维多利亚："的大举措，要有勇气因为我已不再在这里但请决不辜负你的记忆力对我的消失"尼古拉斯：安全，在轻环绕箱免费的恐惧和痛苦我的质疑态度已帮我找到意思是我人生的又维多利亚的实质最后，我觉得在和平与女孩在我的梦想现在我来这里它的完全清楚我发现什么所有这一切都意味着如果我死在明天我就是所有权因为我相信之后，我们正在经历精神继承

What little Wind there was blew me towards the Land; and thus, having found two or three broken Oars belonging to the Boat, and besides the Tools which were in the Chest, I found two Saws, an Axe, and a Hammer, and with this Cargo I put to Sea; For a Mile, or thereabouts, my Raft went very well, only that I found it drive a little distant from the Place where I had landed before, by which I perceiv'd that there was some Indraft of the Water, and consequently I hop'd to find some Creek or River there, which I might make use of as a Port to get to Land with my Cargo.

最初一海里，木排行驶相当稳当，但却稍稍偏离了我昨天登陆的地方。至此，我发现，原来这一带的水流直向岸边一个方向流去。因此，我想附近可能会有一条小溪或小河，果真如此的话，我就可驾木排进入港口卸货了。

People always say, Sixiecao find happiness found on the leaf clover grass because it is hoped that the draft on behalf of two leaf clover represents love pay Sixiecao Sixiecao is rare happiness and the meaning is if you pay in the hope that, they will not only find happiness in love with a Sixiecao have true happiness only………… when I took all my happiness Block walking in a strange town, even if I could not find the Sixiecao my life. But I believe that when I was happy ..

人们总说，找到了四叶草就找到了幸福那是因为三叶草的一叶草代表希望二叶草代表付出三叶草代表爱而稀有的四叶草就是幸福四叶草的意思是即使你付出了，希望了，爱了也不一定会找到幸福只有拥有了四叶草才拥有了真正的幸福…………当我带着我全部的幸福，行走于一座座陌生的小镇时，即使我没有找到我生命中的四叶草，可我相信那时的我是幸福的。。

For I fear, lest, when I come, I shall not find you such as I would, and that I shall be found unto you such as all of you would not: lest there be debates, envyings, wraths, strifes, backbitings, whisperings, swellings, tumults: For I fear, lest, when I come, I shall not find you such as I would, and that I shall be found unto you such as ye would not: lest there be debates, envyings, wraths, strifes, backbitings, whisperings, swellings, tumults

我怕我再来的时候，见你们不合我所想望的，你们见我也不合你们所想望的；又怕有纷争、嫉妒、恼怒、结党、毁谤、谗言、狂傲、混乱的事。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。