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High-level expression of secreted Flt-1(2-3) with good bi ological activity were successfully achieved in Pichia.pastoris expression syst em and can be applied to further animal and clinical test.

获得了具有生物学活性的可溶性重组F lt-1受体胞外区2-3loop小片段，为进一步的动物实验和临床应用打下了基础。

The Taenia solium oncosphere 18 gene was cloned and reconstructed into expression vector pYA3341.The recombinant plasmid pYA3341-TSOL18 was finally electro-transformed into an attenuated S.typhimurium definitive host strain of X4500 and the recombinant strain of X4500 (pYA3341-TSOL18) was identified its immunogenicity of expression protein, stability, growth curve, safety in vitro and immunity evaluation by animal experiment in mouse.

克隆并改造TSOL18基因，构建重组质粒pYA3341-TSOL18，电转入鼠伤寒沙门氏菌终宿主菌株X4550，体外鉴定重组菌X4550(pYA3341-TSOL18)表达蛋白的免疫原性、稳定性、生长曲线、安全性和小鼠免疫试验进行评价。

This paper begin with visual apperception, the expression of architecture symbol and identification of the environment ,from the visualeffect of detailed construction specific, road sight, virescence, environment opusculum and so on, materialize the solicitude for human from minute aspects through identification of circumstance and expression of construction signals to form favorable visual sight.

文章从视觉感知、建筑符号的表达、环境的可识别性开始，通过对具体内容，如居住建筑细部、道路景观、绿化布置、环境小品等的视觉效果研究，从细部体现对人的关怀，形成人性化的视觉景观体系。

Methods: The immunohistochemistry was used to investigate the protein expression of p27 in 46 cases of osteosarcoma and 16 cases of osteochondroma. The expression of p27 and histological category and differentiation levels of osteosarcoma were observed.

应用S-P免疫组织化学方法检测46例骨肉瘤和16例骨软骨瘤组织中p27的表达，并观察p27表达在骨肉瘤组织学分型、分化程度等临床指标之间的差异。

Results The expression of MMP-2 was high in OS,was low in osteochondroma,but the expression of TIMP-2 was reversed.

结果 MMP-2在骨肉瘤中高表达，在骨软骨瘤中低表达，而TIMP-2则相反。

CD40/CD40L interactions were also shown to play a significant role in the maintenance of autoimmunity,High-level expression of CD40L and CD40have been detected on monocytes/macrophages from patients with active sgstemic lupus erythematosus,rheumatoid arthritis and other autoimmune diseases,indicating that the expression of CD40/CD40L on monocytes/macrophages could account for the formation of synovitis and osteoclasia.

CD40/CD40L是免疫细胞间的重要信号转导途径，单核巨噬细胞表达CD40与CD40L，分泌许多促炎性因子，参与类风湿关节炎滑膜炎形成、骨和软骨破坏。在系统性红斑狼疮、类风湿关节炎和其他自身免疫病中已检测出高水平表达的CD40与CD40L。

Methods Expression of p16 was examined in 30 cases of normal ovarium, 32 benign ovarian tumor, 8 borderline ovarian tumor and 103 ovarian cancer by using ABC immunohistochemistry. The association between p16 expression and clinicopathology was also analyzed.

用免疫组化 ABC法检测30例正常卵巢、32例卵巢良性肿瘤、8例交界性卵巢肿瘤和 10 3例卵巢癌组织中的抑癌基因 p16蛋白表达情况，分析 p16蛋白表达与卵巢癌分级分期、转移预后的关系。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

To explore (1)whether the EPO is present in gastric carcinoma ;(2)the possible mechanism of its expression;(3) the role of EPO in tumor angiogenesis and its results, we investigated the expression of EPO, HIF-la in gastric carcinoma and marked EC with CD105( monoclonal antibody MS1290 )and CD34 on paraffinic slides. Analysis of the realationship between EPO MVDand HIF-la in human gastric carcinoma.

本实验检测人胃癌及癌旁组织中EPO、HIF-1a的表达，同时进行了小鼠抗人CD105单克隆抗体(MS-1290)标记和CD34标记，分析EPO与微血管密度及HIF-1a表达的关系，以期明了①胃癌组织中是否存在EPO的表达、②表达的途径和机制、③EPO与肿瘤血管生成的关系和可能的后果。

The dissecting of samples in group2 were difficult. The root of pulmonary artery and ascending aorta failed to be unfolded because fibrous tissue was tough, right and left fibrous trigone were too firm to be solved by hand. Cardiac muscle fibers couldn't be stripped along myofibrillar trajectory since they were prone to break because of their friability.

组2的心脏解剖困难，表现为纤维组织坚韧，游离肺动脉非常困难；徒手无法松解左、右纤维三角，肺动脉和主动脉根部的游离非常困难；心肌纤维坚硬、质脆，解剖时容易断离成碎块，无法沿纤维走行方向剥离。

We have battled against the odds in a province that has become increasingly violent.

我们对在一个争夺日益激烈省的可能性。