查询词典 find expression in

To the organ with lowest expression by in vivo injections with Vibro alginolyticus. Then the mature peptide of Lutjanus sanguineus NCCRP-1 was directionally connected the expression vector pET-21a to construct pET-21a-NCCRP recombinant.

PCR结果表明红笛鲷NCCRP-1基因在头肾、胸腺、脾脏、肝脏、肌肉、肠、心脏、鳃中均有表达，但表达量有差异，经注射溶藻弧菌后淋巴样器官NCCRP-1基因的表达量明显升高。

The expression level of FLK1 mRNA was lower (P.01) at 12 h after MACO in DMCI group than that in NDMCI group. Except for at 6 h, there was no significant difference between DMCI and NDMCI group in the expression level of FLT1 mRNA.

结果 DMCI组在脑缺血后各时间点脑缺血半暗带等值区VEGF mRNA的表达水平均显著低于NDMCI组（P.05～0.01），FLK1 mRNA 表达水平在脑缺血后12 h显著低于NDMCI组（P.01），FLT1 mRNA 表达水平在脑缺血后各时间点（除6 h）与NDMCI组差异无统计学意义。

To acquire more information about gene expression at the whole-genome scale,our lab and National Engineering Center for Beijing Biochip Technology worked together and constructed the first genome-wide oligonucleotide microarray of the silkworm.This microarray was used to detect gene expression of 10 tissues/organs,including midgut,integument,head,hemocyte,testis,ovary,anterior/median silk gland,posterior silk gland,fat body and malpighian tubule.In this study,we performed analysis of these microarray data and confirmed the reliability of the results.

为了在全基因组水平上获得基因表达信息，本实验室与生物芯片北京国家工程中心合作，共同定制完成了世界上第一张家蚕全基因组寡核苷酸基因芯片，并利用该芯片对家蚕5龄第3天幼虫的中肠、体壁、头、血液、精巢、卵巢、前中部丝腺、后部丝腺、脂肪体、马氏管这10个组织中的基因表达谱进行了检测。

With the method of reverse transcription-polymerase chain reaction and nest PCR, P80 gene of CSFV is cloned from the cells infected by the strain Shimen of csfv, and connected with eukaryotic expression vector pEGFP-C1. Then recombined eukaryotic expression plasmid pEGFP-P80 of P80 gene is achieved successfully. It builds the basis for expressing and gaining protein p80 in mammiferous cells.

利用反转录PCR和套式PCR技术，从猪瘟病毒Shimen株细胞毒中克隆出P80 基因，并进一步把P80 基因连接在真核表达载体pEGFP-C1 上，成功地构建出P80 基因重组真核表达质粒(pEGFP-P80)，为在哺乳动物细胞中表达并纯化出p80 蛋白奠定了基础。

RESULTS: FGFR1-positive chondrocytes were abundant in the maturative layer and hypertrophic layer, but only few FGFR1-positive chondrocytes were found in the proliferative layer. In the control group, the expression of FGFR1 increased from 6-week-old to 10-week-old rats, and then decreased and went stable. In both young and adult group, the expression of FGFR1 in the experimental group was significantly lower at 4, 6 weeks and higher at 8 weeks than that in the control group, especially in the young group (P.05). But no difference was found between the experimental group and the control group at 2 weeks in both groups. CONCLUSION: FGFR1 may play an important role in the remodling of condylar cartilage induced by occlusal disorders.

结果：FGFR1主要表达在大鼠髁突软骨过渡层和肥大层，增殖层很少；正常组大鼠髁突软骨从6周龄到10周龄FGFR1表达逐渐增强，10周龄后降低，并保持在一个稳定水平；成年组和幼年组实验4、6周时FGFR1的表达均显著低于对照组，实验8周时的表达高于对照组(P.05)，幼年组尤为明显，实验2周组与对照组无显著差异。

Our previous studies have shown that ubiquitous overexpression of HmgD caused hemocytes proliferation and the formation of melanotic tumors in the Drosophila larvae. To clarify the mechanism of melanotic tumors appearance,we also generated RT-PCR analysis to test the transcriptional changes of PPAE and Spn27A,which encode the key factors in the phenoloxidasecascade pathway from larvae samples of mid-third instar,late-third instar and prior to pupation relative to wild type flies.Our result showed that in the HmgD overexpression mid-third instar larvae,the expression level.of Spn27A was lower than wild type,otherwise the expression level of PPAE was higher than wild type.It suggests that overexpression of HmgD affects the PO cascade pathway.

本研究组前期工作发现，过量表达HmgD引起果蝇幼虫体内血细胞增生，并出现黑色素瘤，为探讨HmgD过量表达导致了血细胞增生即形成黑色素瘤的分子机制，本研究分别提取三龄中期、后期和相对于野生型化蛹前时刻的幼虫的RNA，首先检测了编码酚氧化酶级联反应途径中的两个关键基因PPAE，Spn27A在突变体中的转录发生变化，结果发现，Spn27A在三龄中期的表达量低于野生型，而PPAE的表达高于野生型，表明过量表达HmgD影响了酚氧化酶级联反应途径，低水平的Spn27A不足以抑制PPAE的活性，活化的PPAE催化了PPO的活性，使其转变成有活性的PO，从而促进黑色素的生成，使果蝇体内产生黑色素瘤。

Results: The expression of HO-1 mRNA in macrophage cell was significantly enhanced by the melilotus suaveolens extract. The result of Western blot showed that the expression of HO-1 protein was significantly increased after the intervention of melilotus suaveolens extract.

结果：臭苜蓿根提取物明显升高巨噬细胞HO-1的mRNA表达水平，Western blot结果显示，臭苜蓿根提取物干预后HO-1的蛋白表达水平也明显升高。

Methods To mensurate the expression of CD44 and CD24 of MCF-7 in diverse incubation circumstance,and the expression of CD44 and CD24 of primary breast cancer cells from human breast cancer tissue by flow cytometry.

方法采用流式细胞技术检测在不同的培养条件下MCF一7中CD44和CD24的表达情况以及乳腺癌组织原代细胞中的CD44和CD24 表达情况。

To mensurate the expression of MDR1 of different breast cancer cells from human breast cancer primary cells and MCF-7 breast cancer cell line,and the kill effect of chemotherapy drug with different breast cancer cells in vitro,and the change of the proporation and the expression of MDR1 of breast cancer stem cells after chemotherapy.

测定人乳腺癌标本和MCF-7中不同细胞亚群的MDR1表达情况；并测定化疗药物在体外对人乳腺癌原代细胞和MCF-7中不同细胞亚群的杀伤作用，以及在化疗药物干预后，乳腺癌干细胞的含量和MDR1表达的变化情况。

METHODS: 8-Bromo-cAMP and sodium nitroprusside were added into the media to raise the concentrations of cAMP and cGMP of cultured cardiomyocytes, in situ hybridization and immunohistochemistry methods were used to identify the mRNA and protein expression of bFGF. The amount of bFGF mRNA and protein expression were detected by computor imaging analysis system MIAS exe.

分离培养大鼠心肌细胞，加入8-溴-cAMP和硝普钠，升高细胞内cAMP、cGMP浓度，用原位杂交及免疫细胞化学的方法观察细胞内bFGF的表达，图象分析检测bFGF表达量的变化。

Diabetes is a social disease that affects several million people worldwide.

糖尿病是一个社会性疾病，全世界有数百万人罹患此病。

I'll call you on Friday to see if we can reschedule our luncheon meeting at your convenience.

我星期五会给您打电话在您方便的时候我们重新安排我们的午餐约会。