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To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中，苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变，并筛选在自生生长时期、共生生长时期的表型，以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因，我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法，发现lsrA基因主要在根瘤侵染区开始表达，表达时序也在侵染阶段左右，但晚于bacA基因表达；LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体，nifA和fixK基因的转录水平降低，nifH基因的转录被完全阻断，因此LsrA蛋白为根瘤发育所必需，是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因，并研究了它的调节特性。oxyR突变后，苜蓿中华根瘤菌对过氧化氢敏感性提高，适应性降低。

The result showed that there was no expression in the normal control group and an apparent expression of ERK1/2 and Elk in the asthma group. A rather dense expressing of ERK1/2 was at bronchiole and mucous membrane, sub mucous membrane, smooth muscle, cytoplasm and nuclei of the out layer of the smooth muscle cell and an expression of positive fiber at submucous membrane.

结果发现正常肺内没有发现ERK1/2和Elk的表达，而哮喘时肺内有明显的ERK1/2和Elk表达，ERK1/2较密集表达在小支气管和细支气管的粘膜层、粘膜下层、平滑肌层和平滑肌外层细胞的胞浆和胞核中，也可见粘膜下层有阳性纤维表达。

Except for 2μmol/L Verapamil,other concentrations ofCAs and CaMAs could decrease mdr1 mRNA expression level of K562/VCRcells,indicating significant difference from those of control group(CAs:P＜0.05;CaMAs:P＜0.01),but the changes of mdr1 mRNA expression levelcaused by CaMAs had negative dose-responses relationship with CaMAsconcentration(P＜0.05),while the changes of mdr1 mRNA expression levelinduced with CAs had no dose-responses relationship with the CAsconcentration.

除〓的Ver外，其它浓度的CAs和CaMAs均可降低K562/VCR细胞mdr1 mRNA表达水平，与对照组相比，存在着明显差异（CAs：P＜0.05；CaMAs：P＜0.01）；并且CaMAs各浓度间引起的mdr1 mRNA表达水平的变化也存在着差异（P＜0.05），呈负的量效关系，而CAs各浓度间引起的mdr1 mRNA表达水平的变化无明显差异。

Sequence structure of a cloned gene is an important factor to determine its expression level in E.coli. In this paper, we studied the expression of a fragement of HIV-1 gp120 gene and a fragement of choler toxin A subunit gene in E.coli using a new expression strategyoptimizing the gene sequence as a whole.

外源基因的序列结构是影响外源基因在大肠杆菌中表达的重要因素，我们采用基于"外源基因全序列结构优化"的新的表达策略，研究了HIV-1 gp120-801和霍乱毒素A亚单位(CTA-246)基因在大肠杆菌中的表达情况。

The expression level of metabolitic enzyme (CYP1A1, CYP1B1) and its correlation with cell differentiation /apoptosis were studied as well. The influence of resveratrol in cell cycle was determined with FCM. Results 1 Resveratrol not only suppresses the growth of medulloblastoma cells in a dose and time related fashion but also induces cell differentiation and apoptosis. Resveratrol promotes differention of UW228-1,-2 cells to forward glia, UW228-3 and Med-3 cells to neuron. A treatment of resveratrol in the concentration of 100μM for 48hrs comit most of cells die of apoptosis. 2 Among the four cell lines Fas and Caspase-3 were constantly expessed, whereas FasL was absent irrespective to the drug treatment. 3 ICC, RT-PCR, Western-blot hybridization and EROD enzymatic activity assay demonstrated that expression of CYP1A1 is different after treatment with resveratrol in four cell lines, up-regulated in three UW228 cell lines in a dose dependent manner but down-regulated in Med-3 cells. Suppressive effect of resveratrol on CYP1B1 expression is the same among four medulloblatoma cell lines. 4 Cell cycle distribution of UW228-3 was greatly changed after treatment.

结果：1、白藜芦醇以时间和剂量依赖性方式抑制髓母细胞瘤细胞生长，同时促进细胞的分化和凋亡：白藜芦醇促使UW228-1、-2靶细胞向神经胶质细胞方向成熟分化，UW228-3、Med-3细胞向神经元细胞方向成熟分化，以100μM作用48小时效果显著；2、四个细胞系均有Fas和Caspase-3表达，但无Fas-L基因表达和蛋白活性产生，因此难以形成Fas相关性死亡通路；3、白藜芦醇处理前后CYP1A1基因的表达在各细胞系略有不同：UW228-1，-2，-3三个细胞系白藜芦醇以剂量相关性方式上调CYP1A1的表达，而Med-3细胞系，白藜芦醇却抑制CYP1A1的表达；四个细胞系在白藜芦醇处理后CYP1B1的表达均受到抑制；4、UW228-3细胞系经白藜芦醇作用后细胞周期有较大改变，表现在G0/G1期在整个细胞周期中所占的比例增加，而其它时期则相应减少。

The value of a conditional expression is defined as follows: If the effective boolean value of the test expression is true, the value of the then-expression is returned.

一个条件表达式的值定义如下：如果测试表达式的有效布尔值为true，则返回then表达式的值。

If the effective value of the test expression is true, the conditional expression ignores any dynamic errors encountered in the else-expression.

如果测试表达式的有效值为true，条件表达式忽略任何else表达式中遇到的动态错误。

London, 1904 holds that no reasonable man would profess to disprove the Christian miracles historically; that in historical studies we must accept the principle of continuity as set forth by evolution, that the statements of the New Testament are based mainly on Christian experience, in which there is always an element of false theory; that we must distinguish between the true underlying fact and its defective outward expression; that this expression is conditioned by the intellectual atmosphere of the time, and passes away to give place to a higher and better expression.

认为，没有合理的男子自称推翻了基督教历史上的奇迹，这在历史研究，我们必须接受的原则连续性所阐述的演变，该声明的新约主要是根据基督教的经验，其中总有一项内容虚假的理论；，我们必须区分真正的基本事实，其缺陷向外表达；，这言论自由是有条件的智力气氛的时候，和去世给地方更高，更好地表达。

It was found that there was not an obvious correlation of the cumulation of differential expression genes with dominant expression level to the expression of rice heterosis.

分析表明，具显性表达水平的差异表达基因的聚合与杂种优势表现无明显相关性。

In this case, the listener usually has two kinds of response: to ask for clarifications engender negative feelings Finally, when the speaker uses "gaigen" expression to communicate a message, there is no misunderstanding is because: the authority of the speaker the listener knows the speaker is telling his opinion but not about fact And the speaker uses "gaigen" expression to communicate a message, misunderstanding causes because: the discourse maker and the information content was recognized differently (EX: the speaker pay attention to the information, but the listener pay attention to the discourse maker) the communication goal was different (EX: the listener needs the information as a fact, but the speaker offers his own opinion) the use of "gaigen" expression as a discourse maker was differently used by the speaker and listener (EX: A's using of "perhaps" has a huge difference from the one of B) By examining written sentences and written dialogues (2 people talking), the two kinds of language phenomena "representing the determinate information indeterminately" and "representing the indeterminate information determinately" was solved by analyzing the language data carefully.

最后，使用概言表现传递不确定讯息时，而会导致误会产生，是因为下列其中因素之一出现：(1)谈话标帜跟讯息内容认知的不一致（例如其中一方注意的是谈话标帜，而另一方注意的是讯息内容）(2)说者与听者间会话目标的不一致（例如听者所需要的是「事实讯息」，但说者所提供的是个人的「意见讯息」）(3)使用概言表现的用法的不一致（例如甲所使用的「大概」跟乙所使用的「大概」差距太大）。透过分析与归纳的结果，本稿最初提出的「确定的事物却使用不确定的语气描述」、以及「不确定的事物却使用确定的语气描述」之语言现象亦得到了解答。

Hanna: That's over now, isn't it?

都结束了，对吗

You must be ill. You look so pale.

你一定是病了，你的脸色苍白。