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A suitable expression system is required for heterologous expression of human lactoferrin.

目前常用的表达系统中，动物乳腺是人乳铁蛋白基因表达较为合适的器官。

It was shown that the resultant cybrid HL-R cells incorporated with reticulocyte cytoplasts became differentiated 〓 bytalterationcin growth parameters, which might be defined as decancerization:(1) loss of growth ability in soft agar medium;(2) nontumorigenic under heterotransplantation in their filial cellular generation to mude mice;(3) decrease of growth rate, cellular mitoxic index and DNA synthesis rate;(4) decrease or inhibition of c-myc gene expression;(5) expression of human globin gene products in 〓 subculture.

利用C-myc癌基因探针的分子杂交技术，未检测到胞质杂交细胞存在相当于C-myc基因特异的同源序列mRNA，提示杂交细胞癌基因表达严重受抑。

The expression of VEGFmRNA was valued by in situ hybridation method and the expression of Fit-1 and MV count was investigated by using immunohistochemical method.

应用原位分子杂交方法研究VEGFmRNA的表达，应用免疫组化方法研究Flt-1表达及MV的计数。结果：增生性瘢痕和瘢痕疙瘩组织中MV数目多於正常皮肤和扁平瘢痕组织中的MV数目。

Results compared with the normal villi,the quantity of P21 expression has no significant difference,the site of P21 expression in the hydatidiform mole is quite different.

结果 与正常绒毛相比，P21癌基因在葡萄胎组织中的表达量没有显著性差异，表达部位有明显不同。

Some factors, like expression stage in adenovirus and expression level in influenza, is also prominent in some viruses. In HPV and HIV, the hydropathy of the protein also plays its role, although not the main factor.

某些选择因素如腺病毒中基因的表达时相、流感病毒中基因的表达水平是比较明显地影响病毒密码子选择的因素，而在HPV和HIV基因所编码的蛋白的疏水性尽管对于病毒的密码子使用有影响，但不是最主要的因素。

In this study, the amount of ethylene and its key biosynthesis genes expression, typical allelochemical hydroxamic acid concentration and the key regulation genes (BX1 and BX9), as well as the dynamic expression and time of relevant defense key genes of secondary substance metabolism processes were compared in ethephon treatments of different concentration and duration.

为探明外源乙烯利对玉米化学防御作用诱导的浓度和时间效应，本文采用化学和分子生物学相结合的方法，研究了不同浓度乙烯利处理玉米后，叶片乙烯释放量及其合成调控关键基因ACS和ACO表达的变化，典型化感物质丁布的含量及其关键调控基因BX1和BX9表达的变化，以及其他调控次生代谢物的关键基因表达的时间动态。

To investigate the hyperglycemic effect on ICAM-1 expression of BBB, we took a dynamic observation of the expression on endothelium of BBB in SD rats with hyperglycemia for 3 days to 6 months, by immunohistochemical method.

为了解超负荷血糖条件下有否血脑屏障内皮细胞ICAM-1的表达，本研究采用SD大鼠尾静脉注射链脲霉素的方法，建立超负荷血糖模型。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

The positive rate of ER expression was 30 percent and that of ERmRNA expression was 36 percent of hyperplastic tissue, the results were not equal to each other.

ER与ERmRNA在增生组织中的表达阳性率分别为30%、36%，呈现后者高于前者的不一致性。

Results:The positive rates of TSP- 1 mRNA in hypertrophic scars and keloid were lower than those in normal skin and surgical scar. MVC was higher in the cases with negative expression of TSP - 1 mRNA than the ones with positive expression of TSP - 1 mRNA. MVC was higher in the hyperstrophic scar and keloid than in normal skin and surgical scar.

结果TSP-1 mRNA在增生性瘢痕和瘢痕疙瘩中表达的阳性率低于在正常皮肤和外科瘢痕组织中的表达(P＜0.05)；TSP-1 mRNA表达阴性病例组的MVC显著高于TSP-1 mRNA阳性病例组的MCV(P＜0.05)；增生性瘢痕和瘢痕疙瘩组织中微血管数多于正常皮肤和外科瘢痕组织的微血管。

I was up all night because I was in pain, she said.

我整夜，因为我在疼痛，她说。

A crow caws /craoks.

乌鸦叫。 51-论文网