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CA II and AQP5 expression levels were determined by immunofluorescence and immunoblotting, while CA activity and Na, K-ATPase activity by a colorimetric assay. Anhidrotic mice treated by topiramate did not differ from controls in average secretory coil diameter, CA II expression, CA activity and Na, K-ATPase activity.

应用免疫荧光和western blot技术发现：小鼠在托吡酯处理后泌汗减少，碳酸酐酶同功酶II型(carbonic anhydraseII，CAII)蛋白表达未出现显著变化，但汗腺组织胞膜的水通道蛋白-5(aquaporin-5，AQP5)表达量下调。

In summary, our results indicate that hypohidrosis is a frequent adverse effect in children with topiramate therapy. Younger age and hot weather rather than drug dosage are independent risk factors for topiramate-associated hypohidrosis. The resultes also rule out the possibility that topiramate act on its known mechanisms of action, such as potentiating GABA activity at GABAA receptors and antagonizing the AMPA/KA subtype of glutamate receptor in the secretory cells of sweat glands to inhibit sweat secretion. Our data also suggest that topiramate impairs sudomotor function in mice and leads to a significant reduction in AQP5 expression in sweat glands of anhidrotic mice, thus raising the possibility that dysregulation of AQP5 may contribute to topiramate related hypohidrosis. CA inhibition may not be an important contributor since CA II expression and CA activity was intact in anhidrotic mice treated with topiramate.

综合全部实验结果，本研究提示：夏季和低龄是托吡酯引起泌汗障碍的主要危险因素，而托吡酯剂量对泌汗功能的影响较小；托吡酯可以抑制小鼠汗液的分泌，小鼠的年龄并不影响这种抑制作用，但托吡酯对小鼠体温无明显影响；托吡酯并不是通过对GABAA受体及AMPA和KA两种谷氨酸受体亚型的作用而在汗腺分泌细胞抑制泌汗；托吡酯不影响汗腺形态及Na，K-ATP酶活性；汗腺分泌细胞的AQP5功能失调可能与托吡酯引起的泌汗障碍密切相关，而碳酸酐酶抑制作用并不是泌汗功能损害的主要原因。

It appears, therefore, from the above list, that the expression, animal life, is nearly synonymous with the expression, animal heat; for while Food may be regarded as the Fuel which keeps up the fire within us- and Fuel serves only to prepare that Food or to increase the warmth of our bodies by addition from without -- Shelter and Clothing also serve only to retain the heat thus generated and absorbed.

所以，从上面的陈述来看，动物的生命这一个词语可以跟动物的体温作为同义语用：食物，被作为内燃的燃料，——煮熟食物的也是燃料，煮熟的食物自外吞入体内，也是为增加我们体内热量的，——此外，住所和衣服，也是为了保持这样地产生和吸收的热量的。

Because of this, any of others inattentive eyesight or expression will make you feel hurt for no reason, and you always suspect that there are some animosities towards you in these eyesight or expression.

也因此，别人不经意的眼神或表情总是会令你莫名其妙地感到受伤，你总是怀疑那些眼神或表情里有着对你的敌意。

(1) cerebral ischemical reperfusion injury rats'limbs motor function is variable. Acupuncture could promote lims'functional recovery.(2) PCNA masc cells is visible in cerebral ischemical semidarkness region. There is cell regeneration phenomenon. Acupuncture could strengthen injury region's PCNA expression, could profit injury recovery and functional reconstruction.(3) In ischemia semidarkness region for the model group and acupuncture group, PCNA masc cells percentage of 14days group is lower than 7days group. Along with the recovery of injury, cell multiplication is weaken.(4) In cerebral ischemia semidarkness region, there is VEGF masc cells and regeneration phenomenon. Acupuncture could strengthen injury region's VEGF expression, could profit protection after injury and blood vessel regenerate.(5) In ischemia semidarkness region for the model group and acupuncture group, VEGF masc cells percentage of 14days group is lower than 7days group. Along with the recovery of semidarkness region, ischemia and anoxemia state is getting improved, and VEGF is reduce.(6) As there are PCNA and VEGF masc cells in brain injured region, we could conclude that, after brain ischemical reperfusion injury, there are blood vessel regeneration phenomenon. Acupuncture could promote blood vessel regeneration, recovery blood supply sufficiently and quickly, and promote the recovery of brain injury region.(7)The VEGF masc cells percentage of inhibitor group is lower than acupuncture group. It state that the effect of acupuncture promote VEGF is partly depend on the existing of eNOS.

实验结论：(1)脑缺血再灌注损伤后大鼠的肢体运动功能发生改变，针刺可以促进肢体功能恢复；(2)脑缺损伤区可见PCNA阳性细胞，存在细胞再生现象，针刺可以增强损伤区PCNA的表达，有利于损伤的修复和功能重建；(3)针刺组和模型组14d时缺血损伤区PCNA阳性细胞百分比低于7d组，随着损伤逐渐得到修复，细胞增殖现象减弱；(4)脑缺血损伤区可见VEGF阳性细胞，存在内皮型细胞再生现象，针刺可以增强损伤区VEGF的表达，有利于脑损伤后保护和缺血区血管再生；(5)针刺组和模型组14d时缺血损伤区VEGF阳性细胞百分比低于7d组，随着缺血损伤的修复，缺血缺氧状态得到改善，产生的VEGF减少；(6)由于脑损伤区同时出现PCNA阳性细胞和VEGF阳性细胞，前者是增殖细胞的标志，后者是促进血管再生的重要因子，可以推断，脑缺血再灌注损伤后脑内存在血管再生现象，针刺可以促进损伤区的血管再生，更迅速而充分的恢复损伤区的血供，促进脑损伤区的修复；(7)抑制剂针刺组脑损伤区VEGF阳性细胞百分比与针刺组相比有不同程度的降低，说明针刺促进缺血损伤区VEGF表达部分依赖eNOS的存在。

The expression of Bcl-2 and Bax expression was revealed immunocytochemically using the antiserum against Bcl-2 and Bax.

Bcl-2原癌基因蛋白是位于线粒体膜、核膜及溶酶体膜的膜蛋白，可抑制细胞凋亡。

Sarcomeric actin expression was positive on day 10 after differentiation. the positive expression was in plasma. on day 6, plasma and organelle in cells increased, tight junction and intermediate junction were found and there was apophysis in cell surface and microfilament in apophysis.

电镜观察，未分化细胞为圆形或椭圆形，细胞间连接松散，细胞质较少，细胞器不发达；诱导后6 d，细胞间出现紧密连接和中间连接，细胞质增多，部分细胞表面出现突起，突起内可见微丝。

The present study, from the cognitive perspective, takes into consideration the following three points: the cognition of apposition is based on the knowledge of types and categories of things and objects; the original expression and its appositional expression are used as two different expressions for the same thing or event seen from different perspectives; the essential relationship between the two is that, in spite of the identical feature in syntax, they refer to different aspects of the object described out of different considerations.

认知研究表明，同位语表达的认知基础是对事物类别的认识；本位成分和同位成分是对同一事物不同角度的认识；虽然它们的句法地位相同，但在句中所体现的意义和功能有异。

The eukaryotic expression vector PBI121-CRP was constructed, then transformed into peanut (Arachis hypogaea L.) Heiba920 by agrobacterium-mediated transformation for expression. After the molecular detecting, the transformed plants were obtained.

构建真核表达载体PBI121-CRP，利用根癌农杆菌介导法导入黑花生，使其在花生中表达，经分子生物学检测后，获得可表达CRP的转基因花生植株。

To continue the previous work from our laboratory, the purpose of this study is to investigate IGFBP-5 gene expression by BP and ripen areca nut extract in NHOKs . The results in this study suggest that BP and rANE exhibited inhibition effects on IGFBP-5 gene expression both on transcription and translation levels in NHOKs .

本研究发现， BP 及老槟榔萃取物对於正常口腔角质细胞中 IGFBP-5基因之表现均有抑制之效果，且此抑制效果不但发生於基因转录阶段，甚至还持续到转译阶段；类似的结果也在 SCC25细胞中观察到。

The dissecting of samples in group2 were difficult. The root of pulmonary artery and ascending aorta failed to be unfolded because fibrous tissue was tough, right and left fibrous trigone were too firm to be solved by hand. Cardiac muscle fibers couldn't be stripped along myofibrillar trajectory since they were prone to break because of their friability.

组2的心脏解剖困难，表现为纤维组织坚韧，游离肺动脉非常困难；徒手无法松解左、右纤维三角，肺动脉和主动脉根部的游离非常困难；心肌纤维坚硬、质脆，解剖时容易断离成碎块，无法沿纤维走行方向剥离。

We have battled against the odds in a province that has become increasingly violent.

我们对在一个争夺日益激烈省的可能性。