查询词典 find expression in

The CryCI gene and essential regulation elements cut from the plasmid pGF4ABC were inserted into yeast expression vector pPIC9K and plant expression vector pBI 121.1, so we got the secretive yeast expression plasmid pPIC9KBC and plant high-efficiency expression plasmid pGBIF4ABC, Fifty-two His+Muts transformants were obtained after the expression vector pPIC9KBC was introduced into Pichia pasoris, KM7I strain, by electroporation.

将pGF4ABC上的融合杀虫基因CryCI分别克隆到酵母表达载体pPIC9K和植物表达载体pBI121.1上，构建成融合杀虫基因的分泌型酵母表达载体pPIC9KBC和高效植物表达载体pGBIF4ABC。

Transcript level was not obviously different between MSTN and IGF2. The expression of MSTN reached the highest point at 7 day and droped to the lowest point at 7 weeks after birth. The expression of IGF1 arrived at the highest peak at brith, and degraded the half of the birth after 7 days. Then, the change of the IGF1 expression level was no abvious. The expression result of the IGF2 decreased to the least at 1 week and increased the max point at 3 weeks. The change regulation of IGF2 expression was showed as a wave.

其中，IGF-1表达水平最低，MHC2a表达水平最高；IGF2与MSTN表达水平相当； MSTN的表达于7天时表达水平最高，是出生时表达的3倍，随后于14天时急剧下降，于7周时降至最低水平；出生时IGF1的表达水平最高，随后于一周时降至出生时的一半，后期的表达变化不大； IGF2的表达水平于3周时表达最高，是出生时的两倍，在1周时最低。3周后骤然降低，呈现一个波浪型变化；MHC的表达出生时最高，于1周后降至原来的1/7，随后逐渐升高，但变化并不太大。

Results showed:(1) The airway responsiveness of smoke control group was higher than that of normal control group, the airway responsiveness of smoke + exercise group was lower than that of smoke control group;(2) Plasma level of cortisol determined immediately after exercise was higher than that determined before exercise, no significant difference occurs between that determined next day morning after all exercise finished in contrast to that determined before exercise;(3) HE staining showed, there was severe chronic pulmonary inflammatory response in smoke control group, which was reduced in the smoke + exercise group;(4) chronic cigarette smoke downregulated the protein and mRNA expression of BKca in smooth muscles of bronchi and bronchioli, exercise increased the mRNA expression of BKca;(5) chronic cigarette smoke downregulated the protein and mRNA expression of Kv1. 5 in smooth muscles of bronchi and bronchioli, exercise increased both the protein and mRNA expression of Kv1. 5 in contrast to smoke control group in bronchioli and did not effect the expression of Kv1. 5 in bronchi;(6) chronic cigarette smoke induced upregulation of FIZZ1/RELMα in bronchial smooth muscle cells, exercise decreased these effect.

结果：(1)吸烟对照组的气道反应性明显增高，吸烟加运动应激可明显降低大鼠的气道反应性；(2)吸烟运动组运动后血浆皮质醇浓度明显高于运动前，但全部运动结束后次日晨测定的血浆皮质醇浓度与运动前无明显差异；(3)HE染色显示，吸烟对照组肺组织出现明显的慢性炎症反应，吸烟加运动应激组比吸烟对照组炎症反应轻；(4)慢性吸烟可降低大鼠大气道和小气道BKca mRNA和蛋白表达，而吸烟加运动应激可使BKca mRNA表达上调，但对小气道的蛋白表达无影响；(5)慢性吸烟可降低大鼠大气道和小气道Kv1.5蛋白和mRNA表达，在小气道吸烟加运动应激可减轻这种作用，大气道则无作用；(6)吸烟使支气管平滑肌的FIZZ1/RELMα蛋白表达明显增强，而吸烟加运动组的RELMα蛋白及mRNA均明显减弱。

The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 130dB infrasound treatment In 1 day group, Bcl-2mRNA and P53mRNA were expressed lightly in CA1-CA3 areas in hippocampus and dentate gyrus. Blood vessels dilated lightly. In 7d group, Bcl-2mRNA and P53mRNA were more expressed in areas mentioned above. Dilation of capillaries and small vessels was more obvious. Bcl-2mRNA and P53mRNA were strong positive in endothelial cells of vessels. In 14d group, Bcl-2mRNA and P53mRNA expression was obviously increased in pyramidal cells of hippocampus and granular cells of dentate gyrus, showing deep blue staining. a The endothelial cells in some vessels were also strong positive for Bcl-2mRNA and P53mRNA. Dilation of capillaries and small vessels was lessened. In 21d group, Bcl-2mRNA and P53mRNA expression was decreased in pyramidal cells. In 28d group, little change about the expression and distribution of Bcl-2mRNA and P53mRNA was observed. Lots of Bcl-2mRNA and P53mRNA were scattered in cortex of temporal lobe around hippocampus and callosal gyrus area. Dilation of capillaries and small vessels was lessened obviously. The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 90dB infrasound treatment.

次声作用后Bcl-2mRNA和P53mRNA在海马的表达，16Hz、130dB次声作用1d组：两者在海马〓区和齿状回轻度表达，海马内血管轻度扩张；7d组：两者表达在上述区域较1d增多，微血管和小血管扩张较显，血管内皮细胞内可见呈强阳性表达的Bcl-2mRNA和P53mRNA；14d组：海马锥体细胞和齿状回颗粒细胞内Bcl-2mRNA和P53mRNA阳性表达明显增多，呈深兰色，在部分血管内皮细胞内两者强阳性表达，微小血管扩张状态较7d减轻；21d组：锥体细胞胞浆内Bcl-2mRNA和P53mRNA表达减弱；28d组：上述表达及分布与21d组相比无明显改变，海马周围颞叶皮层和扣带回区域亦可见大量散在分布的Bcl-2mRNA和P53mRNA表达，血管扩张状态已明显减轻。90dB次声作用1d-7d后，较对照组无明显变化，14d-21d后轻度表达，28d后，Bcl-2mRNA和P53mRNA表达较强。

He type of TGF β1 expression was related with lymph node metastasis and prognosis of patients. The positivity rate of TGF β1 expression only in mesenchyma in the group on lymph node metastasis was significantly lower than that in no lymph node metastasis, but the positivity rate of TGF β1 expression only in cytoplasma was higher. The prognosis of TGF β1 expression only in mesenchyma was better, but the prognosis of TGF β1 expression only in cytoplasma or both mesenchyma and cytoplasma was poor.

GF β1的阳性表达类型与肺癌的淋巴结转移及预后有关，淋巴结转移阳性组TGF β1的单一间质阳性表达率显著低于淋巴结阴性组，而单一胞浆阳性表达率高于淋巴结阴性组；半年内死亡组的TGF β1单一间质阳性表达率低于5年以上生存组，而间质胞浆均阳性的表达率及单一胞浆阳性表达率则高于5年以上生存组(P均＜0.05)。

The expression of subunits α5,β1,β3 was detected in normal morulas and increased in blastocysts. The expression of subunit α1 was not detected in morulas or blastocysts, but detected only in outgrowing trophocytes. 3. Expression of integrin β3 in irradiated group embryos was weaker than that of normal group. There was no expression of integrin α1 both in morulas and blastocysts in irradiated group and no difference in the expression of subunits α5 and β1 between these two groups.

结果：1、超声波照射组胚胎贴附率、滋养细胞外延生长率均显著低于正常胚胎。2、正常胚胎桑椹胚期即有α5、β1、β3整合素表达，而无α1表达；囊胚期α5、β1、β3表达增强，仍无α1表达；α1只出现在外延生长的滋养细胞。3、超声波照射组胚胎β3整合素的表达明显弱于正常组，且未见α1表达；而α5、β1的表达两组间无差异。

Outcome:(1) Acute ischemic damage may induce the expression of c-fos protein in Cerebral Cortex quickly. Also, the expression increases by the ischemia time delay. It may hint that ischemia injury can rise the ability of nerve cells to tolerate ischemia and anoxia. And the expression level of c-fos can be up-regulated by putting blood of 12 hand Jing Points. So we think that treated by this therapy can rise the irritability of brain to resist the development of ischemia and enhance the ability to plerosis.(2) Acute ischemic damage may induce the expression of HspVO in Cerebral Cortex quickly. Also, the expression increases by the ischemia time delay. It may hint that ischemia injury can rise the ability of nerve cells to tolerate ischemia and anoxia.

三 结果(1)急性缺血性脑损伤可快速诱导c-fos蛋白在皮层缺血区脑组织的表达，其表达有随缺血时间延长而逐渐增多的趋势，提示脑的缺血性损伤可应激性地增强神经细胞对缺血、缺氧的耐受和适应能力，而应用井穴放血的干预治疗可明显上调缺血区皮层脑组织c-fos的表达水平，进一步增强脑组织对抗缺血性损伤的应激能力，抵抗缺血性脑损害的进一步发展。

In the present paper, the Sox gene expression analysis of different tissues from the Trionyx sinensis was studied by using RTPCR, and Sox gene fragments of expression from the testicle, brain and spleen were cloned using RTPCR products. The results show that Sox gene has specific expression in the testicle, brain, spleen, cardiac muscle and kindney and hasn't expression in muscle, liver and ovary. The results of clone reveal that the Sox genes of expression in the testicle are TSSox1 and TSSox 4, and those are TSSox2 and TSSox4 in the brain, and that is TSSox4 in the spleen. This suggests that the Sox gene act important role not only on the sex determination, but also on the development of neural system, immunocyte system and the differentiattion of male germ cell.

本文采用RT—PCR技术，研究了中华鳖不同组织Sox基因的表达，并通过PCR直接克隆法，分析了来自睾丸、脑和脾组织中的Sox基因序列结果表明在中华鳖的成体组织中，Sox基因在脑、心肌、肾、脾和雄性的睾丸组织中均有不同程度的表达，而在肌肉、肝脏和雌性的卵巢中则无表达，显示该基因具有组织表达特异性克隆分析显示，在睾丸组织中表达的是TSSox1和TSSox4基因，而在脑组织中表达的是TSSox2和TSSox4基因，脾组织中表达的是TSSox4基因此结果表明Sox基因不仅在性别决定中起作用，还可能在神经系统、免疫系统多种组织中起重要作用

I draw people through the test, understand the different classes of children aged people in figure painting tests on the following characteristics: 1, small classes can not find child care because of the graphic match, the majority of child care both in the overall figures are still details to chaos The lines to express disorder. 2, Zhongban due to the continuous improvement of cognitive, has been able to find a match in the minds of the graphics, and has been in the minds of the graphic expression, but the details painted on the expression of often rough. 3, taipans child care whether or details on the whole, are more perfect expression, but the large class paintings by children of mothers are the embodiment of their own, can not distinguish between adults and children.

笔者通过画人测验，了解不同年龄班的幼儿在人物画人测验上有以下的特点：1、小班幼儿由于找不到匹配的图形，大部分幼儿无论是在人物整体上还是细节上都是用混乱无序的线条来表达。2、中班由于认知的不断提高，已经能在头脑中找到相匹配的图形，并将头脑中已有的图形表达出来，但是在细节描绘上表达的往往比较粗略。3、大班幼儿无论是在整体上还是细节上，表达的都比较完善，但是大班幼儿所画的妈妈都是自己的化身，不能区分大人和小孩。

I'd leave ninety-nine leave them all behind to find you i'd leave ninety-nine leave them all behind to find you it's dark and lonely and the path is unclear can't move my feet because i'm frozen with fear then you say, my child, my child i am always here, i'm at your side i'd leave ninety-nine leave them all behind to find you i'd leave ninety-nine leave them all behind to find you you're never too far down i promise you'll be found, i'll reach into the mud, the miry clay pursue you to the end, like a faithful friend, nothing in this world, can keep me away, i'd leave ninety-nine leave them all behind to find you i'd leave ninety-nine leave them all behind to find you...

我离开99 离开他们背后找到你我离开99 离开他们背后找到你这是黑暗和孤独，目前尚不清楚的路径不能移动我的脚，因为我害怕被冻结然后你说，我的孩子，我的孩子我一直在这里，我在你身旁我离开99 离开他们背后找到你我离开99 离开他们背后找到你你永远都不会太远了我答应你会发现，我要达到的泥土，在泥泞的粘土追求你的目的，就像一个忠实的朋友，没有在这个世界上，可以让我离开，我离开99 离开他们背后找到你我离开99 离开他们背后找到你。。。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。