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The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清，进行以下检测：(1)原子吸收法测血清锌浓度；(2)测胸腺、脾脏脏器重量并与大鼠体重比较，计算脏器指数；(3)HE染色对胸腺、脾脏进行病理观察；(4)原位末端标记法测胸腺、脾脏中淋巴细胞的自发凋亡，并通过图像分析进行比较；(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达；(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达；(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

But there are no correlations between gene expression and gene expression specificity or gene expression level.

有趣的是，基因的组织特异性和基因的表达量与基因表达的进化则可能没有相关性。

Results VEGF-C and VEGFR-3 are all expressed on both mesenchymal cells and vascular endothelium of the embryos during early period. In middle and latter period, VEGFR-3mRNA is mainly expressed on cell plasm of alimentary canal, but it has no expression on vascular and lymphatic endothelium; The expression of VEGFR-3 is extensive and positive expression is seen on the vascular and lymphatic endothelium.

在胚胎中、后期消化道组织中VEGF-C主要表达于消化管壁组织细胞以及肠系膜内，在血管和淋巴管内皮细胞则无表达；VEGFR-3亦表现为广泛的表达，于血管和淋巴管内皮细胞可见阳性表达，并随着胚胎的发育，其在淋巴管内皮细胞的阳性表达率明显增加。

In the functional mechanism, we should use the technique "use the DNA Microarray to build the gene expression difference chart" to lead to the combination of Traditional Chinese Pharmacy and gene expression difference chart to study. Meanwhile,we can establish a totally new method of screening modern Traditional Chinese Pharmacy based on the gene expression difference chart, it can really make the modernization and internationalization of Traditional Chinese Pharmacy.

在中药作用机制方面，应采用&利用基因芯片建立基因表达差异谱&技术，将中药与基因表达差异谱有机地结合起来进行研究，同时以基因表达差异谱为基础建立一种全新的现代中药筛选方法，使中药真正实现现代化、国际化。

The results of SS investigating indicated that the SS protein expression in leaf at seedling stage was high comparatively, when at jointing and heading stage, SS expression was low in leaves and stems, along with sorghum development, since flowering stage, SS expression increased sharply, decreased at dough stage.

2对SS表达检测结果表明，甜高粱苗期叶片中SS蛋白质表达量较高，拔节、抽穗期叶片和茎中SS蛋白质表达量较低，随着高粱的生长发育，开花以后叶片和茎中SS的表达量普遍升高，腊熟期略有下降。

To better understand the mechanism of sucrose accumulation, the correlation of sucrose content and sucrose phosphate synthetase、sucrose synthase expression was analyzed by monitoring the expression of SPS in leaves and stems.(1) The results of SPS investigating indicated that the sucrose content, the expression of SPS in leaves and stems were increased along with the growth of sorghum, reached the highest level at grain filling stage, and decreased at dough stage.

1对SPS表达检测结果表明，在高粱的生长发育过程中，茎中蔗糖含量、叶片和茎中SPS蛋白质表达量持续上升，灌浆期达到最高，腊熟期略有下降，它们之间的变化趋势基本一致，甜高粱茎蔗糖含量与叶片SPS蛋白质的表达相关系数为0.895，与茎SPS蛋白质的表达相关系数为0.781；甜高粱和普通高粱相比，其叶片中SPS表达量差别不明显，而甜高粱茎中SPS表达量明显高于普通高粱。

Results The activation of NF-κB, proliferation response, and expression of IL-4 and I L-5 mRNA and protein in T lymphocytes stimulated by PMA were significantly hig her than those of their blank control (P.01), while those indexes of T l ymphocytes stimulated by PMA and PDTC simultaneously were significantly lower th an those stimulated by PMA alone (P.01). The apoptotic index of T lympho cytes stimulated with PMA were significantly lower than that of their blank cont rol (P.01), and the apoptotic index of asthmatic guinea pig T lymphocytes stimulated with PMA and PDTC simultaneously were significantly higher than that stimulated by PMA alone (P.01). The significant positive correlations w ere found between the activation of NF-κB and the proliferation (r=0.64, P.001), and the expression of IL-4 and IL-5 mRNA and protein of T lymph ocytes, respectively (r=0.55-0.68, P.001). There was also signific ant negative correlation between the activation of NF-κB and apoptosis of T l ymphocytes (r=0.62, P.001). Conclusions NF-κB may participate in the signal conduction of PKC regulated proliferation, apoptosis and expression of IL-4 and IL-5 of T lymphocytes in asthma.

结果加入PMA培养的哮喘组T淋巴细胞NF-κB的活化、细胞增殖反应、IL-4和IL-5的mRNA和蛋白质的表达均显著高于其空白对照(P.01)，而同时加入PMA和PDTC培养的哮喘组T 淋巴细胞以上指标均显著低于只加入PMA培养的哮喘组T淋巴细胞(P.01)；加入PMA 培养的哮喘组T淋巴细胞的凋亡指数显著低于其空白对照(P.01)，而同时加入PMA 和PDTC培养的哮喘组T淋巴细胞凋亡指数显著高于只加入PMA培养的哮喘组T淋巴细胞(P 。01)。T淋巴细胞NF-κB的活化与增殖反应呈显著正相关(r=0.64，P.00 1)，与IL-4和IL-5的mRNA和蛋白质的表达也均呈显著正相关(r=0.55-0.68，P 。001)，而与凋亡指数呈显著负相关(r=-0.62，P.001)。

OBJECTIVE To evaluate the expression of epidermal growth factor receptor as a molecular target for oropharyngeal squamous cell carcinomas measured by automated quantitative analysis and its significance of EGFR expression, especially nuclear EGER expression in the prognosis of cancer.

目的 评价自动定量分析法(automated quantitative analysis， AQUA)测定表皮生长因子受体(epidermal growth factor receptor EGFR)在口咽部鳞状细胞癌组织中的表达水平，了解其在判定预后中的价值。

Expression patterns of genes differentially expressed in starch metabolism and cell cycle during endosperm development7 DAP kernals, 15 DAP endosperms and 25 DAP endosperms of B73, ae/wx and sh1 were used to study the expression patterns of 18 differentially expressed genes in sugar metabolism and 9 differentially expressed genes in cell cycle. We found that the expression patterns of Pul, SBEⅡa, UGPase, SUS3, SSⅡa in sh1 and Sh2-1, SUS3, SSⅡa in ae/wx were altered, revealing that these genes may interact with each other.

碳水化合物代谢基因和细胞周期基因随胚乳发育的变化以B73、ae/wx、sh1授粉后7天种子、15天和25天胚乳为材料，分析18个糖代谢差异基因和9个细胞周期调节相关的差异基因的表达模式，研究发现sh1突变体中Pul、SBEⅡa、UGPase、SUS3和SSⅡa基因表达模式发生改变，ae/wx突变体中Sh2-1、SUS3和SSⅡa基因表达模式发生改变，揭示了突变体对这些基因的巨大影响，反映了它们可能存在相互作用。

In a word, we extracted first cotyledon of buckwheat total RNA, by method of RT-PCR and 3RACE, get the C terminal cDNA sequence of PI from Ukraine buckwheat, and do some bioinformatics analyse on this cDNA sequence of PI from buckwheat, such as gene characteristics, expression mode. All of this will be help for the forward research on functional protein and high performance of procaryon expression or eukaryon expression.

综上所述，我们利用RT-PCR和3′RACE技术首次从吉乌一号甜荞第一对子叶中克隆获得荞麦蛋白酶抑制剂基因并对其基因特性、表达模式进行生物信息学分析，同时构建了该基因序列羧基端原核表达载体，为今后进一步研究荞麦功能性蛋白的分子机制及高效原核、真核表达载体的构建奠定了基础。

Liapunov—Schmidt method is one of the most important method in the bifurcation theory.

Liapunov—Schmidt方法是分叉理论的最重要方法之一。

Be courteous -- even when people are most discourteous to you .

要有礼貌──即使当別人对你最不礼貌的时候。