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Expression rate induced by thrombin was 50.88% and PAC?1 re?expression was 54.55%. It is concluded that the ability of recovered lyophilized platelets added with reversible activation?inhibitors, DMSO and trehalose to aggregate and coagulate has showed no significant difference as compared with FPRP. The reversible activation?inhibitors can decrease CD62p expression of lyophilized platelets, and may enhance their survival ability and prolongate survival time. Therefore the efficiency of lyophilizing platelets can be improved based on this freeze?drying procedure.

添加血小板可逆性激活抑制剂，海藻糖和DMSO后的冻干血小板，其聚集活性和促凝血功能与新鲜血小板无明显差异，血小板可逆性激活抑制剂降低了冻干血小板的CD62p表达，增强了冻干血小板的生存能力，因而延长了其生存时间，因此可以该冻干方法为基础进一步提高冻干保存血小板的效率。

Among 15 cases with pylic embolus , there were 8 casesnon-expression,4 cases low expression, and 3 cases high expression of P27 in carcinoma t

PCNA 中等表达和高度表达32例，其中单纯根治性切除6例，术后复发6例，根治性切除加术后介入治疗26例，术后 3 中文摘要复发12例，二者之间有统计学差异0功。05人 9、P27与PCNA在肿瘤组

Those have secret touch and the narrate in detail with beautiful form, or expression is natural grain appearance, or expression is geometrical grain appearance, or expression is abstract grain appearance, figure and recorded hunan person racily to be happy to decorate, be good at decorate and

那些具有神秘感和形式美的铺陈，或表现为自然纹样，或表现为几何纹样，或表现为抽象纹样，形象而生动地记录了楚人乐于装饰、善于装饰和

PartⅠ The Expression and Location of HCK Gene During the Development of Rattus Heart Objective To investigate the mRNA expression of HCK gene in the developing rattus heart and to analyze the relationship between HCK gene expression and the heart development.

目的检测HCK基因在大鼠胚胎心脏发育过程中mRNA表达的变化，分析其与心脏发育的关系。

The ability to modulate the expression of multiple genes by tetracycline and its derivatives is likely to regulate the timing and level of gene expression, quantitatively induce the expression of toxic protein, increase therapeutic efficacy and safety, and provide a safe and regulatable way for gene therapy.

CHO-Tet-On细胞株的建立，可利用四环素及其衍生物调节多种外源基因的表达，有效调控基因表达的时间和水平，定量诱导毒性蛋白的表达，增加治疗的疗效和安全性，有望为基因治疗提供一条可控的安全途径。

Egr-1 mRNA and Egr-1 protein hadn't been found in the normal vein. The expressions of Egr-1 mRNA and Egr-1 protein had biphasic changes. By reverse transcription-PCR and in situ hybridization, we found that the level of Egr-1 mRNA rose at 1 hour after graft, the expression of Egr-1 mRNA was (35±7)%. Decline at hour 6, 24 and day 3, the positive rates of Egr-1 mRNA were (8±2)%,(8±6)% and (8±4)% respectively. Reincrease at day 7, a peak at day 28, the positive rate of Egr-1 mRNA was (45±6)%(compared with other phase, P＜0.01). At day 42, the expression of Egr-1 mRNA declined again. Immunohistochemical staining and Western blot revealed Egr-1 protein had expressed at hour 2 early phase, the expression of Egr-1 protein was (30±5)%, and until to hour 6. The level of Egr-1 protein was decrease at hour 24 and day 3, the positive rates were (7±3)% and (7±8)% respectively.

结果 自体静脉移植后，Egr-1 mRNA和Egr-1蛋白的表达呈双相变化，即移植后1 h， Egr-1 mRNA表达迅速升高，阳性率为（35±7）%，6 h、24 h及3 d时下降到较低水平，阳性率分别为（8±2）%、（8±6）%和（8±4）％，7 d时又再升高，28 d时达高峰，阳性率为（45±6）%，此与其余各时点比较差异均有统计学意义（P＜0.01），42 d时，Egr-1 mRNA的表达再次下降；移植早期（2 h）即有Egr-1蛋白的表达，阳性率为（30±5）%，并持续至6 h，24 h～3 d表达下降到较低水平，阳性率分别为（7±3）%和（7±8）%，7 d时又再升高，至移植后28 d，Egr-1蛋白的表达阳性率达到高峰，为（40±9）%，此与其余各时点比较差异有统计学意义（P＜0.01）。

Two relevant sites of enzymatic digestion were added to the mTNF-α by PCR. The mTNF-α was linked to the 3'end of m/〓 in pGEX4T-1 vector. The prokaryotic expression vector pGEX4T-1m/〓-mTNF-α was constructed successfully. After induction and expression by IPTG, the expression of two kinds of fusion protein is 15% and 12% of total bacteria proteins respectively. The anti-HCC bifunctional antibodies m/〓-mTNF-α were identified by electrophoresis after the inclusion bodies were purified, denature, renature, re-purified, digested by thrombin and further purified.

采用PCR的方法在mTNF-α的两端加上所需要的酶切位点，将之连接在m／〓的3'端，构建原核表达载体pGEX4T-1 m／〓-mTNF-α，通过IPTG的诱导表达之后，两种融合蛋白的表达量分别占细菌总蛋白的15％、12％，表达产物经包涵体的纯化→变性→复性→纯化→凝血酶酶切→进一步纯化后，可以得到纯度为电泳纯的m／〓-mTNF-α抗肝癌双功能抗体。

BMP-7 expression was lower markly in Lung-kidney Qi Deficiency 、Liver-kidney yin Deficiency、 Qi-yin asthenia、Speen –kidney Yang Deficiency than normal control. BMP-7 expression decreased with Lung-kidney Qi Deficiency 、Liver-kidney yin Deficiency、 Qi-yin asthenia、Speen –kidney Yang Deficiency.It shows that the level of BMP-7 expression may play a role in Zheng differentiation-classification of TCM of the chronic glomerulonephritis.

中医证型与肾间质纤维化程度显著相关，肺肾气虚、肝肾阴虚型纤维化程度最轻，气阴两虚型其次，脾肾阳虚型纤维化程度最重，提示传统中医辩证分型与纤维化程度有关，在一定程度上可以反映慢性肾小球肾炎组织学损伤程度，肾间质纤维化程度可作为中医临床辨证参考依据。

The expression changes of TNF-α and p65 mRNA expression were detected by reverse transcription-polymerase chain reaction, the TNF-α level in the supernatant was determined by ELISA, and the expression of p65 in AR42J cell lines was detected by Stept Avidin-Biotio Complex.

逆转录－多聚酶链反应半定量法观察肿瘤坏死因子/p65亚单位mRNA表达的变化；ELISA法检测TNF-α在细胞上清中质量浓度的变化；链酶亲和素－生物素－过氧化物酶复合物法检测p65蛋白在AR42J细胞中的表达。

Objective This study was performed to observe the effect of microvascular density,and the expression of MMP-2/TIMP-2 in the subendocardial myocardium of DOCA-salt Hypertensive Rats left ventricle under the intervention of bosentan,and to evaluate the signifance of expression of MMP-2/TIMP-2 and the role of endothelin-1 on the expression of MMP-2/TIMP-2 in DHR heart.

目的探讨基质金属蛋白酶-2(MMP-2)在脱氧皮质酮-盐型高血压大鼠心脏微小血管重塑中作用及其可能的调节机制。

In the days of sailing vessels the crew were afraid they would be becalmed on the ocean.

在使用帆船的时代，船员们担心他们会因为无风而无法在海洋上航行。

As long as foreign donors pay the PA's salary bill, few expect a new intifada.

只要外国继续为巴权力机构的薪水买单，希望发动新暴动的人便寥寥无几。