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It was also found that Cap43 expression in patient with adenocarcinoma was higher than those with squamous cell carcinoma(P.01). Western blot also showed high expression of cap43 in lung cancer. Cap43 expression in mucoid adenocarcinoma was significantly higher than the other pathological types of lung cancer.

免疫印迹也显示，Cap43蛋白在非小细胞肺癌组织中高表达；其半定量结果显示，Cap43蛋白在肺粘液性腺癌组织中的表达量明显高于其它组织学分型肺癌组织(P.01)。

There was a stastical difference of the expression of hTRT protein among well differentiated adenocarcinoma, poor differentiated adenocarcinoma and mucoid carcinoma. And there was a highly significant positive correlation between the expression of hTRT mRNA and hTRT protein. However, the expression of hTRT mRNA and its protein in GC were not related with other clinicopathological parameters including gender, age, location and size of neoplasm, wall invasion, lymph node metastasis and clinical stage.

结果：1。在53例GC中hTRTmRNA及hTRT蛋白表达均显著高于癌旁组织，hTRT蛋白表达在粘液癌与高分化腺癌和低分化腺癌间表达有显著性差异，hTRTmRNA与hTRT蛋白的表达呈显著正相关，而hTRTmRNA、hTRT蛋白的表达与各临床病理参数包括性别、年龄、肿瘤大小、部位、淋巴结转移、浸润深度及临床分期均无相关性； 2。

Methods Specimens of neurofibroma tissue, keloid and normal skin were collected. The distribution and expression of CTGF was measured by immunohistochemistry. The mRNA expression of CTGF, TGF-β1,ColⅠ and ColⅢ was detected by reverse transcription- polymerase chain reaction. Expression of CTGF protein was measured by western blotting.

收集神经纤维瘤组织、瘢痕疙瘩组织及正常皮肤组织标本，免疫组化检测CTGF的表达，逆转录-聚合酶链式反应方法检测组织CTGF、TGF-β1、Ⅰ型胶原及Ⅲ型胶原 mRNA表达，Western blotting检测组织CTGF蛋白的表达。

The larva-specific expression level of the function correlated to celluar components was the highest.The highest specific expression level of molecular function was appeared at neurula stage.A lot of functional specific expression about biological process was also appeared at neurula stage.7.The result showed that the pearson correlation coefficient was 0.896 compared between the sea urchin and Ciona savignyi.

进一步分析发现：在与细胞组件相关的功能特异性表达方面，幼体期的特异性表达量最高；在与分子功能活性相关的功能特异性表达中，神经胚时期的特异性表达量最高；而参与生物过程的功能特异性表达方面，神经胚时期的特异性表达量则最高。7不同物种间转录组分析显示尾索动物海鞘的转录组与棘皮动物海胆的最接近，其相关系数为0.896，而海胆与文昌鱼的相关系数只有0.682，结果表明在进化上文昌鱼可能与脊椎动物更接近。8运用Apache、php、MySQL技术搭建了Widows平台的文昌鱼EST分析数据库。

Construction and expression of the GFP expression vector is to study the expression of exogenous gene in NSC.

干细胞治疗与基因治疗结合，将是攻克很多难治性疾病的突破点。

It was found greater expression of NRP2-1 and NRP2-2 in pituitary, ovary, uterus and oviduct of ruttish Erhualian than corresponding tissues of ruttish Duroc, which suggested enhancing expression of NRP2 was favorable to increasing litter size. But the difference of NRP2 expression was not significant between two breeds, which suggested the key phase that NRP2 affected litter size was not oestrum probably, or NRP2 could enlarge cell signals and finally affect litter size ascribe that it was the receptor of nerve axon guidance factors.

NRP2-1和NRP2-2在发情期二花脸卵巢、子宫、输卵管中的表达量均大于杜洛克相应组织的表达量，推测NRP2表达水平高可能有利于提高产仔数，但两品种间表达差异不明显，推测NRP2影响产仔数的关键阶段可能不是猪的发情期；另外，NRP2 是神经轴突介导因子的受体，这些神经轴突转导的信号往往有级联放大的功能，虽然NRP2-1和NRP2-2在二花脸和杜洛克卵巢、子宫、输卵管中的表达差异不大，但二花脸有可能通过神经系统转导信号的级联放大最终引起一些效应激素或蛋白的急剧增加，从而提高排卵率或产仔数。

Scanning each character in the expression to separate and record operators, operational symbols and data types of operators, when expression being correct in syntax, recording interpreter executive order based on precedence of operator, a general expression interpreter is designed. The interpreter not only is used for numerical value and string computation, but also is easy to extend its functions, and when batch converting data, it could catch run error and continue to successive data.

2通过对公式的一次扫描，记录操作数、操作符号和操作数的赋值特性，并在语法正确的情况下，记录公式基于算符优先级的解释执行顺序，设计了通用公式解释器，它不仅可以进行数值运算、字符运算，而且可以对使用的函数库方便地进行扩充，在批量进行数据转换时，可以记录运行时错误，而不影响后续数据的执行。

Except is a set operator which returns a query expression that has all the elements of a query expression but not in another query expression.

除了是一家集商的查询表达式返回一个具有所有内容，包括在查询表达式，但在另一个查询表达式没有。

Producing PHAs,and then were orientationally inserted into a prokaryotic expression vector pBV220. By analyzing the bioactibity and function of their products, the biological function of phaA, phaB and phaC was confirmed. These have been done in our laboratory. To make better use of the three genes by plant bioreactor, two groups of different plant expression vectors that can be used to produce PHAs in different plants were constructed, and the transformation of PHA in Oxalis triangnlaris has been preliminarily reseached.The following is the main results:Three plant expression vectors pBI121-A,pBI121-B and ubquiting-C, which can be used to be transformed into Oxalis triangularis were respectively constructed by using the phaA, phaB and phaC.

菌株的亚克隆基因组片段中，分离出phaA、phaB和phaC三个基因片段，定向克隆至原核表达载体pBV220，通过对其生物活性与功能分析，确证了基因phaA、phaB和phaC的生物学功能；为了通过植物生物反应器对这三个基因进一步研究利用，本实验利用这三个基因构建了两组可用于不同植物生产PHA的真核表达载体，并对紫叶酢浆草进行PHA基因遗传转化的初步研究，获得主要结果如下：利用克隆的phaA、phaB、phaC三个基因分别构建了可用于转化紫叶酢浆草表达载体pBI121-A、pBI121-B、uBquiting-C；可用于玉米转化的种子特异性表达载体p7S-A、pBI121-S-B、uBquiting-C。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

London based fashion photographer Matt Irwin might be is the next big thing in fashion photography.

总部位于伦敦的时装摄影师马特欧文可能会是下一件大事的时装摄影。

Based on IMC system,the response would be better if the model matched more satisfactorily.

由于系统基于内模控制设计，故模型匹配度越高，系统响应越好。