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Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

Our results indicate that the plant can only abscise fruits oviposited by parasitic wasps before pollinating wasps in fig-fig wasp mutualism, but do not abscise the fruits parasitized by wasps ovipositing simultaneous or later than pollinating wasps, implying that abscission can not sufficiently maintain the fig-fig wasp mutualism.

结果表明：脱落机制并不能完全阻止非传粉小蜂的寄生，榕树只能选择性地脱落掉先于传粉小蜂产卵的榕果。这也同时表明维持榕树与榕小蜂互利共存的机制不仅仅只有榕果的脱落机制，可能还存在其它未被发现的机制。

Fig 1 At the experimental side, two weeks after operation (HE ×100) Many pieces of cartilage cells and spindle-shaped mesenchymal cells were found, by light microscope Fig 2 At the experimental side, twelve weeks after operation (HE ×100) Medullary cavity had formed, which contained hemoblasts and adipocytes, but the trabculae was not typical, by light microscope Fig 3 At the experimental side, twenty-four weeks after operation (HE ×100) The typical cancellous bone had formed, by light microscope Fig 4 At the control side, twenty-four weeks after operation (HE ×100) A large part of CXB was degraded, resorbed and replaced by fibrous tissue, but there was'not any new formed bone, by light mocroscope

图1 实验侧术后2周(HE ×100)镜下可见很多软骨细胞条索和团块生成及大量梭形间充质细胞图2 实验侧术后12周(HE ×100)镜下可见有髓腔形成，内含有造血和脂肪细胞，骨小梁尚不典型图3 实验侧术后24周(HE ×100)镜下可见形成典型的松质骨结构图4 对照侧术后24周(HE ×100)镜下可见大部分CXB被降解吸收，为纤维组织替代，无新骨形成

Fig.5 mp53 mRNA in situ hybridization signal in human HXO-Rb44 cells(Fig.a, experimental group,EG;Fig.b, control group,CG).The signal as violet-colored fine granules localized in the cytoplasm,EG weaker than CG,×1 000;Fig.c, mp53 mRNA dot blot signal,EG weaker than CG

图6～8 人HXO-Rb44药品细胞 p16、p21WAF1及Rb 免疫调查组织化学反应性，a为实验组，b为对照组，免疫调查反应细颗粒位于药品细胞核和药品细胞质，EG强于CG，×1 000；c为Rb、p21WAF1及p16蛋白斑点印迹免疫调查反应性，EG强于CG。

On the membrane of liver parenchymal cell and endothelial cell ×12 000;Fig 4 3 h after Cd-injection:ER extremely dilatant,mitochondria swollen,a lot of Ce-H2O2 precipitation on the membrane of PC,EC and Kupffer＇scell ×15 000;Fig 5 6 h after Cd-injection:in irreversible state,hepato-cytoplasm clear,few organelle,a few Ce-H2O2 precipitation on the membrane of PC and eC ×5 500;Fig 6 24 h after Cd-injection:hepatocyte plasmotorrhexis,membrane of

ce呈点状沉积在肝实质细胞、内皮细胞膜上×12 000；图4 染镉后3小时：ER极度扩张，线粒体膨胀，内外膜模糊不清；见大量Ce沉积在PC、EC和Kupffer＇s细胞膜上×15 000；图5 染镉后6小时：处于不可逆改变状态的肝细胞胞浆清亮，少有细胞器；仍可见少量Ce沉积在PC和EC膜上×5 500；图6 染镉后24小时：可见破碎的肝细胞，胞内膜结构大多已不清晰；未见Ce的沉积×7 000

When fishing a Zulu or Floating worm subsurface, but using a traditional "weightless" technique, you may want to: use a larger hook (Fig. 16), add some weight to your hook such as "Sticky Weight"(Fig. 17) or SuspenStrips, use a swivel and a short leader (Fig. 18) or other technique to cause the lure to sink to the desired depth or to suspend it.

当你想使用软饵 ZULU 或是漂浮型软虫，却需要采用无坠钓法在中层水域施钓，你可以采用大一些的软虫钩（图片16），在钩的中部添加一些重量，如粘铅，（图片17），或是使用八字环，接一小段前导线（图片18），利用可以有的办法，原则上掌握添加重量的比例，使其达到施钓水层或者驻留在某个高度。

FIG. 1 is a front elevational view of a shampoo bottle showing my new design;FIG. 2 is a rear elevational view thereof;FIG. 3 is a left side elevational view thereof

为了让大家比较容易了解，以下将一个例子来解说新式样专利说明书的文字部分，之后再来探讨绘图时必须注意的事项。

Fig/fig pollinator mutualistic systems are exploited by non-pollinating fig wasps.

榕树与传粉榕小蜂的互惠共生体系被多种非传粉榕小蜂利用。

Of the 26 lesions with pontine and bulbar involvement, three had no association with other lesions (Fig 2). Twelve were associated with tegmental and superior cerebellar peduncular extension, and six showed corticospinal tract involvement in continuity with an MDJ lesion. In one patient with additional telencephalic lesions, the right side of the pons was hyperintense on long TR/TE images that did not cross the midline, suggestive of an arterial lesion (Fig 3). In two patients with pontine tegmental lesions, there was an associated middle cerebellar peduncle and deep cerebellar white matter involvement (Fig 4). Two others had associated cervical lesions.

在26处脑桥延髓区病灶中，3处病灶仅限于脑桥延髓区，其它部位无病灶（图2）；12处病灶还同时伴有背盖部和小脑上脚/结合臂处病灶；6处病灶为与中脑-间脑接合处病灶相连的皮质脊髓束受累；还有1例患者除端脑见病灶外，右侧脑桥于T2像也见高信号病灶，但未超越正中线，提示可能系动脉病变所致（图3）；2例脑桥背盖部病灶的患者同时伴有小脑中脚/小脑脑桥脚和小脑深部白质受累（图4）；还有2处脑桥延髓区病灶还同时伴有颈髓病灶。

I don't think Wang can win the Cy Young because he's not high-profile enough.

我不认为WANG会得到赛扬奖，因为他还没有那麼高的掌握性。

Preparation of the prescription of gu yan was divided into drug granule and capsule by the primary study of preparation. Nux vomica was made into capsule according to 0.33g per granulation and the other was made into drug granule according to 15g per bag.

制剂研究初步确定"骨炎方"的制剂分为颗粒剂与胶囊剂两部分，将"骨炎方"中马钱子制成胶囊、余药按一定工艺制成颗粒剂，颗粒剂15克/袋，胶囊0.33克/粒。