查询词典 field frame

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

We are taking the field in a concentrated frame of mind and the right approach in our heads.

我们集中精神开始了比赛，并且取得了正确的结果。

We are taking the field in a concentrated frame of mind and the right approach in our heads.

在球场上我们集中注意力，尽量不犯错误。

In the present embodiment the output signal has one frame for each field of the input signal.

在本实施例中，该输出信号对于所述输入信号的每个场具有一帧。

Specification: One kind of bitstream which contains all types of Macroblocks and blocks in the frame and field.

说明：一种具有在场和帧图中出现所有宏块类型和子块类型的编码比特流。

Based on this test, more detailed research of the flow pattern and flow frame and flow field in differ...

因此，分析研究阀门段水流急变分离流的流态及其变化规律，对理论研究和工程设计实践都具有重要意义。

Specification: One kind of bitstream which contains all types of Macroblocks and blocks in the frame and the field.

说明：一种具有在场和帧图中出现所有宏块类型和子块类型的编码比特流。

Therefore,a new subject,urban forest ecology,and correlative new concept frame in the field formed.

城市森林生态学的形成使人们从生态系统的角度审视城市的发展，并把生物环境即人类及所有植物和动物的群体视为这个系统的一个要素，引出了&城市森林&的概念。

Through the introduce of citizenship ,this paper tries to build the analysis frame of "field—right—system".

通过分析，本文认为在现代民族国家中，福利制度设置应以公民的社会权利为根基，以实现公民的社会权利为目的。

To handle this there is a 16-bit frame type field in the Ethernet header.

以太帧头中有个16比特的帧类型区域控制此项。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。