查询词典 fiddler duck

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

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18 When I see a bird that walks like a duck and swims like a duck and quacks like a duck, I call that bird a duck.

当我看到一只飞鸟走路像鸭子、游泳像鸭子、叫声像鸭子，我就会称那只鸟为鸭子

Of course, at this time the duck will not be "lonely duck", as far as I know, wild duck are generally gregarious, and a nest hatched are often eight, so here we have a duck call "eight ducks."

当然此时的鹜不会是"孤鹜"，据我所知，鹜一般都是爱群居的，一窝孵出来往往都是八只，因此鹜在我们这里亦有一种称呼"八鸭"。

The American writer and poet James Whitcomb Riley is credited with the observation that "when I see a bird that walks like a duck and swims like a duck and quacks like a duck, I call that bird a duck".

"当我看到一只鸟像鸭子一样走路，像鸭子一样游泳，像鸭子一样嘎嘎叫，我就把这只鸟叫作鸭子。"此话据说出自美国作家和诗人詹姆斯惠特科姆赖利。

To establish a method to differentiate Muscovy duck parvovirus from goose parvovirus, primers GPVF/R1 and MDPVF1/R1 were designed according to the genomic sequences of GPV and MDPV to amplify the virus culture and nucleotide of GPV, MDPV, duck plague virus, duck hepatitis virus, duck reovirus, canine parvovirus and feline panleucopenia virus.

根据番鸭细小病毒和鹅细小病毒基因组的非同源序列各设计了1对引物MDPVF1/R1和GPVF1/R1，建立了一种PCR方法，用该PCR方法分别对GPV、MDPV、鸭瘟病毒、鸭肝炎病毒、鸭呼肠孤病毒、犬细小病毒和猫泛白细胞减少症病毒的病毒培养物及其核酸进行扩增。

The results show that : pig species include Large White, Landrace, Duroc pigs and swine; cattle varieties of Simmental, Shorthorn and Brahman cattle, of Jersey cattle; sheep varieties Boer goats; duck, duck varieties, Cherry Valley Duck, Shaoxing Duck; Chicken varieties are emerging sanhuang, Guangxi top soil chicken, Ma top emerging chicken, black plume bamboo silk chickens and other species.

研究结果表明：（1）猪的品种主要包括大白猪、长白猪、杜洛克猪和香猪；牛的品种有西门塔尔牛、短角牛、婆罗门牛、娟姗牛；羊的品种有波尔山羊；鸭的品种有北京鸭、樱桃谷肉鸭、绍兴麻鸭；鸡的品种有新兴三黄鸡、广西土顶鸡、新兴麻顶鸡、黑羽竹丝鸡等品种。

Take French Champagne Plum Juice Stewed Duck Chest for example. The duck chest is imported from Australia. Fry the duck chest and stew with champagne, plum juice, Japanese sake, and soy sauce for 30 minutes. Repeat the cooking skill three times and then roast the dish. Finally, add the Balsamico vinegar into the dish. The duck absorbing the sauce tastes tender and succulent.

其中法式的香槟梅汁煨鸭胸，选用从澳洲进口的真空包装生鸭胸肉，热锅后不沾油直接将富油脂的鸭皮放入锅中煎，翻面后再煎红肉，以烙烧的方式封住其肉汁美味，并放入以香槟、梅汁、清酒与少许酱油煮滚的酱汁里关火焖煮三十分钟，重复此步骤三次后再放入烤箱烘烤，再以Balsamico义式陈年老醋拉出优雅线条，甘甜的鸭胸肉充分吸入酱汁的风味，吃起来肉质香嫩，鲜美滋味迷人。

The combination of traditional cooking,nutrition and health caring,and modern food manufacturing technology will make deep process of such duck products possible as crispy fried duck,eight-treasure sauced duck and duck floss ,which will enrich the varieties,better the r.

将传统烹饪、营养保健与现代食品高新技术相结合发展鸭肉制品深加工，制成酥香鸭、八珍酱鸭、鸭肉松等制品，必将大大丰富鸭肉制品的种类，提高产品的科技含量和档次。

Nanjing person eats duck variety a lot of, 6 have have roast duck and saline teal to wait for food of a few duck toward the table of period monarches, ming Taizu Zhu Yuanzhang " solar eclipse roast duck ".

南京人食鸭花样很多，六朝时期帝王们的餐桌上已经有烤鸭和盐水鸭等几道鸭馔，明太祖朱元璋&日食烤鸭一只&。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。