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METHODS By means of cell culture and flow cytometry we studied the effect of fluid nitrogen cryopreservation on the expres sion of MHC Ⅰ antigen of smooth muscle cells and fibroblasts of aortic homografts of ra ts.

采用细胞培养技术和流式细胞仪技术研究液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞主要组织相容性抗原Ⅰ类表达的影响。

RESULTS Expression rate of MHCI antigen of cultured smooth muscle cells and fibroblast was（88.7±4.3）%,（84.8±2.0）%respectively;and that of the revived smooth muscle cells and fibroblasts of aortic homografts after cryopreservation was（89.4±3.5）%,（81.9±2.7）%respectively.

结果 正常培养的大鼠主动脉平滑肌细和成纤维细胞MHCⅠ类抗原表达的阳性率分别为（88.7±4.3）%和（84.8±2.0）%；液氮低温保存的大鼠主动脉平滑肌和成纤维细胞在复苏后MHCⅠ类抗原表达的阳性率分别为（89.4±3.5）%和（81.9±2.7）%。

RESULTS Expression rate of MHCI antigen of cult ured smooth muscle cells and fibroblast was (88.7±4.3)%,(84.8±2.0)%respectively; and th at of the revived smooth muscle cells and fibroblasts of aortic homografts after cryoprese rvation was (89.4±3.5)%,(81.9±2.7)%respectively.

结果正 常培养的大鼠主动脉平滑肌细和成纤维细胞MHC Ⅰ类抗原表达的阳性率分别为(88.7±4.3)％和(84.8±2.0)％；液氮低温保存的大鼠主动脉平滑肌和成纤维细胞在复苏后MHC Ⅰ类抗原表达的阳性率分别为(89.4±3.5)％和(81.9±2.7)％。

Objective:To investigate the expression of cyclinD1,P16 and cyclinA1 in the fibroblasts of keloids and hypertrophic scars,and explore the relationship between the genesis of two different kinds of scars.

目的：观察瘢痕疙瘩和增生性瘢痕中成纤维细胞的cyclinD1？p16和cyclinA1的表达，探讨其表达的差异与两种不同类型瘢痕的形成之间的关联性？

Some cell dropped into the cavity and became free. Thrombosis or part organization could be seen. The internal elastic layer became thin, disappear or broken. In internal and middle layer existed fibroblasts, fibrocytes and collagen. Some of the wall indicated hyaline change, soomth muscle cell decreased greatly. The massive inflammatory cells invaded the middle and external layer. There were many foam cells in the capsule tissue. Cytoplasm was filled with fatty tissue and cholesterol. some cavities were full of thrombosis. Some thrombosis was fibrosis, the bottom was organization. The surface of the thrombosis existed red blood cell and librae.(2)Elatic fibrila staining: the internal elastic menbrane almost completely disappeared, the intact internal elastic menbran could be seen in the new small vessels.

动脉瘤管壁厚薄明显不均，全层或局部区域显著变薄向外膨出，内皮细胞空泡变性或坏死脱落，部分内皮细胞剥离并突入管腔成游离状，可见血栓形成及部分血栓机化；内弹力板变薄、消失或突然中断；在内膜及中膜部位主要为纤维母细胞、纤维细胞和大片胶原；部分动脉瘤壁呈均质状玻璃样变，平滑肌细胞明显减少；中膜和外膜可见大量的炎性细胞浸润；瘤壁组织有纤维母细胞、纤维细胞、大片胶原成分及较多泡沫细胞，胞浆内充满脂类物质及胆固醇结晶；部分动脉瘤腔内充满血栓，有的血拴已经纤维化，血栓基部机化，血栓表面有红细胞和纤维素。

The effect of muscarinic receptors agonist-carbachol on conjunctival fibroblasts'proliferation was studied by Cell Proliferation ELISA.

Brdu酶联免疫吸附试验检测毒蕈碱受体激动剂carbachol对结膜成纤维细胞增殖的影响。

Objective To search for the best approaches to transfect plasmid pEGFP-N1 into human fibroblasts.

目的 采用3种不同的方法将质粒pEGFP-N1转染人成纤维细胞，从中寻找最佳的稳定转染的方法。

Mouse fetal tissues could be cut into uniform small pieces by scissors cutting and needle grinding, which grew well in tissue culture and great amounts of cells grew from the attached small tissue clump to form primary mouse embryonic fibroblasts monolayer.

将这些组织块培养48h，大量的梭形细胞从贴壁小组织块的边缘游离出的，细胞密度大，细胞生长旺盛，很快形成原代小鼠胎儿成纤维细胞单层。

Two ribozymes can efficiently inhibit the synthesize of α1 and procollagen on Hypertrophic Scars-derived fibroblasts.

本研究在于已认识到α1及型前胶原合成可较大程度的抑制疤痕的增生。

In the exudates from wounds, IL-1 was detected. IL-1 is capable of promoting proliferation of fibroblasts and excretion of collagenase, which induces inflammation and fever and promote wound healing. Inflammation is a defense reaction of the body.

同时，在局部创伤渗出液中测出含有IL-1，而IL-1可促进纤维细胞增殖及促进分泌胶原酶，引起炎症和发热，促进创面愈合，而炎症反应被认为是机体的防御反应，因此MEBO具有抗感染、止痛、促进创面愈合和减轻瘢痕形成等作用。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐