查询词典 fibroblasts

The endothelial cells cocultured with fibroblasts from scar had greater proliferation rate than that from normal skin.

另外，Transwell 共培养结果发现，增生性瘢痕来源的成纤维细胞具有显著促进血管内皮细胞增殖的作用。

To explore the reciprocal effects of host-tumor interaction, we developed a system to assess the gene expression patterns and the diversity of gene expression of ERK and PI3K pathway in CAOV3 human ovarian cancer cells and primary carcinoma-associated fibroblasts cocultured with each other for 48 hours with RT-PCR.

方法1。采用逆转录聚合酶链反应的方法分别检测交互作用48小时后的卵巢癌相关成纤维细胞与卵巢癌细胞中基因表达的改变，CAFs及CAOV3细胞外信号调节激酶通路的ERK，PI3K基因表达的变化；2。

To confirm the cells' bone marrow origin, chimeric mice were createdby the rescue of irradiated C57BL/6 mice with marrow fromROSA26, a congenic line expressing lacZ. I/RC resulted in a large population of spindle-shaped fibroblasts containing lacZ.

为了证明这些细胞的骨髓源性，我们利用ROSA26对C57BL/6系小鼠进行照射制作了嵌合子小鼠模型（一种能表达lacZ的同类系），从中发现I/RC产生了大量含有lacZ的纺锤形纤维母细胞。

After 3-8 passages, the oral CAFs and the normal mucosa fibroblasts from tissue culture were observed under light microscope. Vimentin,α-SMA and Cytokeratin protein were detected by immunohistochemistry SP method on glass coverslips. VEGF, TGF-β1, MMP-9, Tenascin-C mRNA were analyzed by semi-quantitative RT-PCR.

用组织块培养法获得原代CAFs和正常粘膜成纤维细胞，0.25%胰蛋白酶消化传代和纯化细胞；通过形态学观察和免疫细胞化学染色对口腔CAFs和NMFs进行鉴定：采用半定量RT-PCR检测口腔CAFs和NMFs 4种基因的表达。

By using cytoplasmic fusion techniques, 4977-bp mtDNA deletion from skin fibroblasts of a CPEO patient was introduced into r0 cells and created the so-called cybrids.

再者，本实验室先前已建立细胞质融合的技术，可将CPEO病人的细胞中所带有之4977-bp断损突变送入r0细胞中。

After 5 - 15 passages, these fibroblasts were exposed to various concentrations of CSE for various durations.

目的 探讨香烟烟雾提取物对原代培养的皮肤成纤维细胞生长和增殖的影响。

To further elucidate the mechanism of BMP in regulating cell differentiation and to provide basis for BMP gene therapy, this study centers on the effects of BMP2 gene transfection on fibroblasts and contains the following works:① A 1.2kb fragment containing full length human BMP2 coding region was inserted into pBK-CMV (expressed both in protocaryon and eukaryon) by using predetermined orientation methods, resulting in phagemid expression vector for BMP2 (pBK-B2).② pBK-B2 was transfected into NIH3T3 cells by using LipofectAMINE.

为了进一步从分子水平探讨BMP对细胞分化的调控作用，并为BMP基因疗法的建立提供依据，本实验围绕BMP2基因转染对成纤维细胞的生物学作用进行了以下几个方面的工作：①将含有人BMP2全长编码序列的基因片段(1.2kb)定向克隆于噬菌粒表达载体pBK-CMV中，成功构建了人BMP2噬菌粒表达载体pBK-B2。

Assay of FAH enzyme activity in skin fibroblasts is possible but not readily available.

皮肤成纤维细胞的FAH酶活检测是可行的但不是现成的。

RESULTS:①Amputated arm of Chinese fire-bellied newt could be fully regenerated in ten weeks, during which process great amounts of skin fibroblasts could be observed in the limb buds.

结果： ①东方蝾螈断肢后10周左右肢体成功再生。肢体再生过程中，肢芽中出现大量皮肤成纤维细胞。

METHODS By means of cell culture and flow cy-tometry we studied the effect of fluid nitrogen cryopreser-vation on the expression of MHCⅠantigen of smooth mus-cle cells and fibroblasts of aortic homografts of rats.

采用细胞培养技术和流式细胞仪技术研究液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞主要组织相容性抗原Ⅰ类表达的影响。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐